A Validated Reversed-Phase HPLC Method Toward Quantification of Some Water-Soluble Vitamins and Preservatives in Pharmaceutical Samples

Document Type : Research Article


Department of Chemistry, Shahreza Branch, Islamic Azad University, Shahreza, Isfahan, I.R. IRAN


In this research, a simple, rapid, and reliable method was developed for isolating and quantifying the primary preservative compound (sodium benzoate (NaB)) and some water-soluble vitamins such as ascorbic acid (C) and five vitamin B compounds, including thiamine hydrochloride (B1), riboflavin-5-phosphate sodium (B2), nicotinamide (B3), pantothenic acid (B5), pyridoxine hydrochloride, (B6) in multivitamin syrup. An ODS column (temperature 35 °C) and a UV detector were used. The mobile phase was pH 3.0 phosphate buffer-methanol at a flow rate of 1.0 mL/min in a gradient elution approach. The analytes mentioned above were separated in 40 min. Method validation was reached by evaluating the detection limit (LOD) and quantification limit (LOQ), accuracy, and instrument precision. The data obtained in real sample analysis agreed with the declared values. The data obtained for under stress multivitamin syrup sample (forced degradation) confirmed no interference effects in the quantification of analytes.


Main Subjects

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