An Innovative Approach: Soft Plasma-Modified Polycaprolactone-Alginate Nanofiber Bioactive Glass with Silver Nanoparticles and PRP for Bone Tissue Regeneration

Document Type : Research Article


1 Faculty of Biomedical Engineering, Islamic Azad University, Science and Research Branch, Tehran, Iran

2 Department of Biomedical Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 National Institute of Genetic Engineering and Biotechnology, Tehran, Iran

4 Applied Biotechnology Research Center, Baqiyatallah University of Medical Science, Tehran, Iran

5 Dental School, Shiraz University of Medical Sciences, Shiraz, Iran


This study aimed to assess the potential of Platelet Rich Plasma (PRP) and extracted platelets from rabbits in promoting bone regeneration by utilizing bioactive glass (BG) and chitosan/gelatin membranes. The objectives were to accelerate the healing process, enhance biocompatibility, and provide anti-inflammatory properties. Silver nanoparticles with a size range of 8 to 10 nm were incorporated into the scaffold to prevent microbial growth. The findings indicate that PRP injection into the lesion or in combination with synthetic BG can accelerate bone cell growth and regeneration, while avoiding inflammation or infection. Moreover, the mechanical performance of the regenerated tissue was enhanced, and favorable biological properties were observed. The results demonstrate that PRP and BG can effectively treat bone lesions by promoting cell adhesion and early bone tissue regeneration. The data suggests that an increase in total porosity leads to a corresponding increase in overall porosity, with S4 exhibiting the highest overall porosity of over 70%. Furthermore, as total porosity increases, the percentage of apatite formation also increases, with S4 displaying the highest formation percentage of over 48%. Additionally, the incorporation of BG and chitosan/gelatin membranes, along with silver nanoparticles, enhances the biocompatibility of the material. Further research is warranted to explore the clinical potential of this approach.


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