Diesel Degradation and Bioemulsifiers Production Using Bubble-Column with a Microbial Consortium Isolated from Hydrocarbon-Contaminated Soil

Document Type : Research Article


1 CONACyT-Instituto Tecnológico Superior de Tierra Blanca. Av. Veracruz S/N Esq. Héroes de Puebla, Colonia Pemex. C.P. 95180. Tierra Blanca, Veracruz, MÉXICO

2 2Instituto Tecnológico de Ciudad Madero, Av. 1o. de Mayo esq. Sor Juana Inés de la Cruz s/n Col. Los Mangos C.P.89440, Cd. Madero Tamaulipas, MÉXICO


Diesel is composed of various toxic compounds that can have a negative influence on the environment including plants, microorganisms, and even groundwater being used for cultivation and human consumption. Diesel oil biodegradation kinetics was investigated using bubble-column reactor and microbial consortium isolated from a hydrocarbons spill site and were assessed by gas chromatography. The purpose of this study was to demonstrate the importance of intrinsic microorganisms used to degrade diesel. 93.84% of the diesel got consumed in the bubble-column reactor after 15 days of culture. The consortium showed the ability to produce emulsifiers using diesel oil as its only carbon and nitrogen source (hydrocarbonclastic). This study showed that the hydrocarbonclastic consortium isolated from polluted soil has the metabolic tools for diesel degradation (as a single carbon and energy source), and the capacity to produce bioemulsifiers in a bubble column reactor.  Microbial consortium and bioemulsifiers produced in this research have the potential to be used in the cleanup processes of polluted soil with hydrocarbons such as diesel.


Main Subjects

[1]Obayori O.S., Ilori M.O., Adebusoye S.A., Oyetibo G.O., Omotayo A.E., Amund O.O,Degradation of Hydrocarbons and Biosurfactant Production by Pseudomonas sp. Strain LP1, World. J. Microb. Biot., 25(9):1615-1623 (2009).
[2] Okoh A.I.,Biodegradation Alternative in teh Cleanup of Petroleum Hydrocarbon Pollutants, Biotechnol. Mol. Biol. Rev., 1(2): 38-50 (2006).
[3] Hong S.H., Ryu H., Kim J., Cho K.S.,Rhizoremediation of Diesel-Contaminated Soil Using teh Plant Growth-Promoting Rhizobacterium Gordonia sp. S2RP-17, Biodegradation., 22: 593-601 (2011).
[4] Harrison A.B., Ripleya M.B., Dartb R.K., Bettsa W.B., Wilsona A.J.,TEMPEffect of Protein Hydrolysate on teh Degradation of Diesel Fuel in Soil, Enzyme. Microb. Tech., 26:388-393(2000).
[5] Ganesh A., Lin J., Diesel Degradation and Biosurfactant Production by Gram-Positive Isolates, Afr. J. Biotechnol., 8(21): 5847-5854(2009).
[6] Ta-Chen L., Chang J.S., Young C.C., Exopolysaccharides Produced by Gordonia Alkanivorans Enhance Bacterial Degradation Activity for Diesel, Biotechnol Lett30:1201-1206(2008).
[7] Bento F.M., de Oliveira C.F.A., Okeke B.C., Frankenberger W.T.,Diversity of Biosurfactant Producing Microorganisms Isolated from Soils Contaminated with Diesel Oil, Microbiol. Res., 160(3):249-255(2005).
[8] Kumar M., Leon V., De Sisto M.A., Ilzins O.A.,Enhancement of Oil Degradation by co-culture of Hydrocarbon Degrading and Biosurfactant Producing Bacteria, Pol. J. Microbiol., 55(2):139-146(2006).
[9] Thavasi R., Subramanyam N.M., Jayalakshmi S., Balasubramanian T., Ibrahim M.B., Biosurfactant Production by Azotobacter Chroococcum Isolated from teh Marine Environment, Mar. Biotechnol. 11(5):551-556(2009).
[10] Ron E.Z., Rosenberg E., Biosurfactants and Oil Bioremediation, Curr. Opin. Biotech., 13: 249-252(2002).
[11] Venosa A.D., Zhu X., Biodegradation of Crude Oil Contaminating Marine Shorelines and Freshwater WetlandsSpill Sci Technol B, 8:163-178(2003).
[12] Pornsunthorntawee O., Wongpanit P., Chavadej S., Abe M., Rujiravanit R., Structural and Physiochemical Characterization of Crude Biosurfactant Produced by Pseudomonas Aeruginoa SP4 Isolated from Petroleum-Contaminated Soil, Biores. Technol. 99:1589-1595(2008).
[13] Thavasi R., Jayalakshmi S., Balasubramanian T., Ibrahim M.B., Production and Characterization of a Glycolipid Biosurfactant from Bacillus Megaterium Using Economically Cheaper Sources, World. J. Microbiol. Biotechnol., 24(7):917-925(2008).
[14] Kumar M., Leon V., Materano A.D.S., Selvin J., Anto T.T., Rajeetha R.T., Natarajaseenivasan K., Biosurfactant Production and Hydrocarbon Degradation by Halotolerant and Thermotolerant Pseudomonas sp., World. J. Microbiol. Biotechnol., 24(7):1047-1057(2008).
[15] Rahman K.S.M., Banat I.M., Thahira J., Thayumanavan T., Lakshmanaperumalsamy P., Bioremediation of Gasoline Contaminated soil by a Bacterial Consortium Amended with Poultry Litter, Coir Pith, and Rhamnolipid Biosurfactant, Biores. Technol., 81(1):25-32(2002).
[17] Kokare C.R., Kadam S.S., Mahadik K.R., Chopade B.A.,Studies on bioemulsifier Production from Marine Streptomyces sp. S1.Ind. J. Biotechnol., 6(1): 78-    (2007)
[18] Margesin R., Labbe D., Schinner F., Greer  C.W., Whyte L.G., Characterization of Hydrocarbon-Degrading Microbial Populations in contaminated and Pristine Alpine Soils, Appl. Environ. Microbiol. 69:3085-3092(2003).
[19] Kasai Y., Takahata Y., Hoaki T., Watanabe K., Physiological and Molecular Characterization of a Microbial Community Established in Unsaturated, Petroleum-Contaminated Soil, Environ. Microbiol. 7: 806-818(2005)
[20] Kaplan C.W., Kitts C.L., Bacterial Succession in a Petroleum Land Treatment Unit, Appl. Environ. Microbiol., 70:1777-1786 (2004).
[21] Röling W.F., Milner M.G., Jones D.M., Fratepietro F., Swannell R.P., Daniel F., Bacterial Community Dynamics and Hydrocarbon Degradation During a Fieldscale Evaluation of Bioremediation on a Mudflat Beach Contaminated with Buried Oil, Appl. Environ. Microbiol. 70:2603-2613(2004).
[22] Yazdian F., Shojaosadati S.A., Nosrati M., Hajiabbas P., Khosravi K.M., On-Line Measurement of Dissolved Methane Concentration During Methane Fermentation in a Loop Bioreactor, Iran. J. Chem. Chem. Eng. (IJCCE), 28(4): 85-93(2009).
[23] Lizardi‐Jiménez M. A., López Sánchez E. Y., Gómez‐de‐Jesús, A., García‐Cruz N.U., Hernández‐Flores C.I., Sinkhole‐Native Oil‐Degrading Microbial Consortium for Hydrocarbon‐ Polluted Seawater RemediationCLEAN–Soil, Air, Water, 44(8): 1046-1050 (2016)
[24] Toren A., Navon-Venezia S., Ron E. Z., Rosenberg, E., Emulsifying Activities of Purified Alasan Proteins from Acinetobacter Radioresistens KA53, Appl. Environ. Microb. 67(3):1102-1106 (2001).
[25] Michaud L., Giudice A.L., Saitta M., De Domenico M., Bruni V., Teh Biodegradation Efficiency on Diesel Oil by Two Psychrotrophic Antarctic Marine Bacteria During a Two-Month-Long Eperiment, Mar. Poll. B., 49(5):405-409(2004).
[26] Singh C., Lin J.,Isolation and Characterization of Diesel Oil Degrading Indigenous Microrganisms in Kwazulu-Natal, South Africa, Afr. J. Biotechnol. 7(12):      -      (2008).
[27] Angeles O., Medina-Moreno S. A., Jiménez-González A., Coreño A., Lizardi-Jiménez M. A., Predominant Mode of Diesel Uptake: Direct Interfacial Versus Emulsification in Multiphase Bioreactor, Chem. Eng. Sci.  165: 108-112 (2017).
[28] Denis B., Pérez O. A., Lizardi-Jiménez, M. A.,  Dutta, A., Numerical Evaluation of Direct Interfacial Uptake by a Microbial Consortium in an Airlift Bioreactor, Int. Biodeter. Biodegr. 119: 542-551 (2017).
[29] Shirdam R., Daryabeigi Zand A., Mehrdadi N. Removal of Total Petroleum Hydrocarbons (TPHs) from Oil-Polluted Soil in Iran, Iran. J. Chem. Chem. Eng. (IJCCE), 28(4): 105-113 (2009).