Response Surface Methodology and Artificial Neural Networks (ANNs) (RSM) Pectin Extraction from Banana Peel: A Modeling and Optimization Approach

Document Type : Research Article


School of Chemical Engineering, Jimma Institute of Technology, Jimma University, Jimma, ETHIOPIA


In the present study, the extraction of pectin from the banana peel (Musa sp.) was optimized using an artificial neural network and response surface methodology on the yield and degree of esterification obtained using microwave-assisted extraction methods. The individual, quadratic and interactive effect of process variables (temperature, time, the liquid–solid ratio, and pH) on the extracted pectin yield and DE of the extract were studied. The results showed that a properly trained artificial neural network model was found to be more accurate in prediction as compared to the response surface model method. The optimum conditions were found to be the temperature of 60oC, extraction time of 102 min, the liquid–solid ratio of 40 % (v/w), and pH of 2.7 and within the desirable range of the order of 0.853. The yield of pectin and degree of esterification under these optimum conditions were 14.34% and 63.58, respectively. Temperature, time, liquid–solid ratio, and pH revealed a significant (p < 0.05) effect on the pectin yield and degree of esterification. Based on the value of methoxyl content and degree of esterification the extracted pectin was categorized as high methoxyl pectin. Generally, the findings of the study show that banana peel can be explored as a promising alternative for the commercial production of pectin.


Main Subjects

[1] Azad A.K.M., Isolation and Characterization of Pectin Extracted from Lemon Pomace during Ripening. Journal of Food and Nutrition Sciences, 2(2): 30 (2014).
[2] Constenla D., Lozano J.E., Kinetic Model of Pectin Demethylation, Latin American Applied Research, 33(2): 91–95(2003).
[3] Fakayode O.A., Abobi K E. Optimization of Oil and Pectin Extraction from Orange (Citrus Sinensis) Peels: A Response Surface Approach, Journal of Analytical Science and Technology, 9(1): (2018). 1186/s40543-018-0151-3
[4] Girma E., Worku T., Extraction and Characterization of Pectin from Selected Fruit Peel Waste, 6(2): 447–454 (2016).
[5] González-Montelongo R., Gloria Lobo M., González M., Antioxidant Activity in Banana Peel Extracts: Testing Extraction Conditions and Related Bioactive Compounds, Food Chemistry, 119(3): 1030–1039 (2010).
[6] Happi Emaga T., Ronkart S.N., Robert C., Wathelet B., Paquot M., Characterisation of Pectins Extracted from Banana Peels (Musa AAA) under Different Conditions Using an Experimental Design, Food Chemistry, 108(2): 463–471 (2008).
[7] Jafari F., Khodaiyan F., Kiani H., Hosseini S.S., Pectin from Carrot Pomace: Optimization of Extraction and Physicochemical Properties, Carbohydrate Polymers, 157: 1315–1322 (2017). 10.1016 /j.carbpol.2016.11.013
[8]  Joel J.M., Barminas J.T., Riki E.Y., Yelwa J.M., Edeh F., Extraction and Characterization of Hydrocolloid Pectin from Goron Tula (Azanza garckeana) Fruit. World Scientific News, 101(June), 157–171 (2018).
        Retrieved from
[9] Khamsucharit P., Laohaphatanalert K., Gavinlertvatana P., Sriroth K., Sangseethong K., Characterization of Pectin Extracted from Banana Peels of Different Varieties, Food Science and Biotechnology, 27(3): 623–629 (2018).
[10] Liew S.Q., Chin N.L., Yusof Y.A., Extraction and Characterization of Pectin from Passion Fruit Peels, Italian Oral Surgery, 2: 231–236 (2014a).
[11] Liew S.Q., Chin N.L., Yusof Y.A., Extraction and Characterization of Pectin from Passion Fruit Peels, Agriculture and Agricultural Science Procedia, 2: 231–236 (2014b).
[12] Manh B., Nguyen N., Pirak T., Cogent Food & Agriculture Physicochemical Properties and Antioxidant Activities of White Dragon Fruit Peel Pectin Extracted with Conventional and Ultrasound-Assisted Extraction Physicochemical Properties and Antioxidant Activities of White Dragon Fruit P, Cogent Food & Agriculture, 5(00), (2019).
[13] May C.D., Industrial Pectins: Sources, Production and Applications, Carbohydrate Polymers. J. Ind. Appl. Pectin, 12(1), 79–99 (1990).
[14] Mohamadzadeh J., Sadeghi-Mahoonak A.R., Yaghbani M., Aalami M., Extraction of Pectin from Sunflower Head Residues of Selected Iranian Cultivars, World Applied Science Journal, 8(1): 21–24 (2010).
[15] Mohamed H., Extraction and Characterization of Pectin from Grapefruit Peels, MOJ Food Processing & Technology, 2(1): 31–38 (2016). 2016.02.00029
[16] Mohamed Hasan Z., Extraction and Characterization of Pectin from Various Tropical Agrowastes, ASEAN Food Journal, 2: 143–150 (1995).
[17] Nahar K., Haque M., Nada K., Uddin M., Mansur M.A. Al, Khatun N., Jabin S., Pectin from Ripe Peels of Mango Cultivars, Bangladesh Journal of Scientific and Industrial Research, 52(3): 229–238 (2017).
[18] Nikolova I., Georgieva N., Optimization of the Pectin Extraction from Pomelo Peels by Oxalic Acid and Microwave, Banat?S Journal of Biotechnology, 5(9): 67–73 (2014).
[19] Oliveira T.Í.S., Rosa M.F., Cavalcante F.L.,
Pereira P.H.F., Moates G.K., Wellner N., Mozzetto S.E., Waldron K.W., Azeredo H.M.C.,Azeredo H.M.C., Optimization of Pectin Extraction from Banana Peels with Citric Acid by Using Response Surface Methodology, Food Chemistry, 198: 113–118 (2015).
[20] Owolabi R.U., Usman M.A., Kehinde A.J., Modelling and Optimization of Process Variables for the Solution Polymerization of Styrene Using Response Surface Methodology, Journal of King Saud University - Engineering Sciences, 30(1): 22–30 (2018).
[21] Phaiphan A., Churat S., Doungta T., Wichalin P., Khanchai W., Penjumras P., Effects of Microwave and Ultrasound on the Extraction of Pectin and its Chemical Characterisation of Banana (Musa Sapientum L.) Peels, Food Research, 4(6): 2030–2036 (2019).
[22] Control for Fruits and Vegetable Products (2nd ed.). In: ASEAN Food Journal.
[23] Ranganna S., Handbook of Analysis and Quality Control for Fruit and Vegetable Products, Second Edition (2004).
[24] Sangheetha S., Illeperuma D.C.K., Navaratne A.N., Jayasinghe C., Effect of pH, Temperature and Time Combinations on Yield and Degree of Esterification of Mango Peel Pectin: A Box-Behnken Design Based Statistical Modelling, Tropical Agricultural Research, 30(2): 1 (2018).
[25] Sundarra, A.A., Thottiam Vasudevan R., Sriramulu G., Optimized Extraction and Characterization of Pectin from Jackfruit (Artocarpus Integer) Wastes Using Response Surface Methodology, International Journal of Biological Macromolecules, 106: 698–703 (2018).
[26] Vriesmann L.C., Teófilo R.F., Lúcia de Oliveira Petkowicz C., Extraction and Characterization of Pectin from Cacao Pod Husks (Theobroma cacao L.) with Citric Acid, LWT - Food Science and Technology, 49(1): 108–116 (2012).
[27] Waghmare A.G., Arya S.S., Utilization of Unripe Banana Peel Waste as Feedstock for Ethanol Production, Bioethanol, 2(1): 146-156   (2016).
[28] Wai W. W., Alkarkhi A.F.M., Easa A.M., Optimization of Pectin Extraction from Durian Rind (Durio Zibethinus) Using Response Surface Methodology, Journal of Food Science, 74(8): 637–641 (2009). 10.1111/j.1750-3841.2009.01331.x
[29] Wai W.W., Alkarkhi A.F.M., Easa A.M., Effect of Extraction Conditions on Yield and Degree of Esterification of Durian Rind Pectin: An Experimental Design, Food and Bioproducts Processing, 88(2–3): 209–214 (2010).
[30] Woldu Z., Mohammed A., Belew D., Shumeta Z., Assessment of Banana Production and Marketing in. International Journal of Sciences: Basic and Applied Research, 4531, 283–307 (2015).
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