Comparison and Study of Physicochemical, Nitrogen, Microbial, Antioxidant, Bioactive, and Sensory Properties of Mahyaveh Prepared in Optimal Conditions with Traditional Mahyaveh

Document Type : Research Article


Department of Food Science and Technology, Faculty of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Varamin, I.R. IRAN


Mahyaveh is the name of a traditional Iranian fish sauce made from the fermentation of small and low-consumption fish, anchovies, and sardines, and contains essential amino acids, vitamins, salts, metal ions, and desirable fatty acids such as Docosahexaenoic acid and Eicosapentaenoic acid. Production conditions and fermentation time can be effective in improving nutritional value and improving its health capabilities. The general purpose of this study was to compare and evaluate the physicochemical, nitrogen, microbial, antioxidant, bioactive, and sensory peptides of Mahyaveh prepared in optimal conditions with traditional Mahyaveh. The results showed that the amount of fat, dry matter, ash, fiber, and carbohydrates of Mahyaveh prepared under optimal conditions was significantly (p≤0.05) higher than traditional Mahyaveh. The amount of moisture and protein in traditional Mahyaveh was significantly (p≤0.05) higher than Mahyaveh prepared in optimal conditions. The amount of total nitrogen, formalin nitrogen, amino nitrogen, proteolysis of essential amino acids, total phenol, and sensory evaluation score in Mahyaveh prepared under optimal conditions was significantly (p≤0.05) higher than the traditional Mahyaveh sample. The amount of trimethylamine, volatile nitrogen, IC50, and microbial load of Mahyaveh prepared under optimal conditions was significantly (p≤0.05) lower than traditional Mahyaveh. The results of this study showed that by optimizing the production conditions of Mahyaveh, fish sauces with higher nutritional value, greater safety, and more desirable sensory properties can be produced than traditional Mahyaveh.


Main Subjects

[1] AOAC International. Association of Official Analytical Chemists. “Official Methods of Analysis”, 17th ed., AOAC Washington DC (2000).
[2] AOAC International, Association of Official Analytical Chemists International. “Official Methods of Analysis”, 18th ed., Maryland (2002).
[3] Anihouvi V.B., Sakyi-Dawson E., Ayernor G.S., Hounhouigan J.D., Microbiological Changes in Naturally Fermented Cassava Fish (Pseudotolithus sp.) for Lanhouin Production, Int. J. Food  Microbiol., (116): 287-291 (2007).
[4] Beddows C.G, Ardeshir A.G., Daud W.J.B., Development and Origin of the Volatile Fatty Acids in Budu, J.  Sci. Food and Agri., 31(1): 86-92 (2006).
[5] Benson H.J, Microbiological Applications. “A Laboratory Manual in General Microbiology”, McGraw-Hill Science/Engineering/Math; 8th edition (­2001).
[7] Capillas C.R., Gillyon C.M., Horner W.F.A., Determination of Volatile Basic Nitrogenand Trimethyleamine Nitrogen In Fish Sauce By Flow Infection Analysis, Europ. Food Res. and Thecnol., 210(6):434-436 (2000).
[8] Cho Y.J., Im Y.S., Park H.Y., Choi Y.J., Quality Characteristics of Southeast Asian Salt-Fermented Fish Sauces, J. Korean Fisheries Society., 33(2): 98-102 (2000).
[9] Choksawangkarn W., Phiphattananukoon S., Jaresitthikunchai J., Roytrakul S., Antioxidative Peptides from Fish Sauce by-Product: Isolation and Characterization, Agrie and Natural Resources., 52: 460-466 (2018).
[10] Colombo M., Zimmermann de Oliveira A.E., Fernandes de Carvalho A.,  Nero L.A., Development of an Alternative Culture Medium for the Selective Enumeration of Lactobacillus Casei in Fermented Milk, Food Microbiology, 39: 89-95 (2014).
[11] Dissaraphong S., Benjakul S., Visessanguan W., Kishimura H., The Influence of Storage Conditions of Tuna on the Chemical, Physical and Microbiological Viscera Before Fermentation Changes in the Fish Sauce During Fermentation, Bioresource Technol., 97 (16): 2032-2040 (2006).
[12] Esmaeili Kharyeki M., Rezaei M., Khodabandeh S., Motamedzadegan A., Antioxidant Activity of Protein Hydrolysate in Skipjack tuna Head, J. Fisheries Sci. and Technol., 7(1): 57-64 (2018).
[13] Faisal M., Islam M., Kamal M., Khan M.N.A., Production of Fish Sauce From Low-Cost Small Freshwater and their Qualitative Evaluation, Progres Agri., 24: 171-180 (2013).
[14] Flores M., Toldra F., Microbial Enzymatic Activities for Improved Fermented Meats, Trends in Food Sci. & Technol., 22(2): 81-90 (2011).
[15] Fukami K., Ishiyama S., Yaguramaki H., Masuzawa T., Identification of Distinctive Volatile Compounds in Fish Sauce, J. Agri and Food Chem., 50(19): 5412-5416 (2002).
[17] Hsu K.­Ch., Lu G.H., Jao Ch.L.,  Antioxidative Properties of Peptides Prepared from Tuna Cooking Juice Hydrolysates with Orientase (Bacillus subtilis), Food Res Int., 42(56): 647‐652 (2009).
[18] Holzapfel W.H., Appropriate Starter Culture Technologies for Small-Scale Fermentation in Developing Countries, Int. J. Food Microb., 75(3): 197-212 (2002).
[19] Je J.Y., Park P.J., Jung W.K., Kim S.K., Amino Acid Changes in Fermented Oyster (Crassostrea Gigas) Sauce With Different Fermentation Periods, Food Chem., (91): 15-18 (2005).
[20] Je J.Y., Lee K.H., Lee M.H., Ahn Ch.B., Antioxidant and Antihypertensive Protein Hydrolysates Produced from Tuna Liver by Enzymatic Hydrolysis, Food Res. Int., 42(9):1266‐1272 (2009).
 [21] Jiang J.J., Zeng Q.X., Zhu Z.W., Zhang L.Y. Chemical and Sensory Changes Associated Yu-Lu Fermentation Process: A Traditional Chinese Fish Sauce, Food Chem, 104(4):1629-1634 (2007).
[23] Kavian F., Nateghi L., Ishaqi M.R., Movahed S., ­Investigation of the Effect of Fish Type, Salt Concentration, and Fermentation Time on Nitrogenic, Microbial and Sensory Compounds of Iranian Fish Sauce (Mahyaveh), Ph.D. Thesis, Islamic Azad University of Varamin-Pishva (2020).
[24]  Kavian F., Nateghi L., Eshaghi M.R., Movahhed S., Optimization Production Conditions on the Amount of Nitrogen Compounds in Iranian Fish Sauce (Mahyaveh), Iran. J. Chem. Chem. Eng. (IJCCE), 41(7):2406 -2416 (2022).
[25] Kilinc B., Cakli S., Tolasa S. Dincer T., Chemical, Microbiological and Sensory Changes Associated with Fish Sauce Processing, Euro Food Res. and Technol., 222: 604-613 (2006).
[26] Kim S., Wijesekara I.,  Development and Biological Activities of Marine-Derived Bioactive Peptides: A Review, J. Funcl. Foods., 2: 1–9 (2010).
[27] Khantaphant S., Benjakul S.,  Comparative Study on the Proteases from Fish Pyloric Caeca and the Use for the Production of Gelatin Hydrolysate with Antioxidative Activity, Comp Biochem Physiol B Biochem Mol. Biol., 151(4): 410‐419 (2008).
[28] Klomklao S., Benjakul S., Visessanguan W., Kishimura H., Simpson B.K., Purification and Characterization Trypsins from the Spleen of Skipjack Tuna (Katsuwonus pelamis), Food Chem., 100(4): 1580-1589 (2007).
[30] Lorenzo J., Munekata P., Dominguez R., Pateiro M., Saraiva J., Franco D., Main Groups of Microorganisms of Relevance for Food Safety and Stability, Innovate Technol. for Food Preserve., 53–107(2018).
[31] Mills S., Stanton C., Hill C., Ross  R.­P., New Developments and Applications of Bacteriocins and Peptides in Foods, Annual Review Food Sci. and Technol., 2: 299–329 (2011).
[32] Matsui R., Honda R., Kanome, M., Hagiwara A., Matsuda Y., Togitani T., Ikemoto N., Terashima M., Designing Antioxidant Peptides Based on the Antioxidant Properties of the Amino Acid Side-Chains, Food Chem., 245: 750-755 (2018).
[33] Mooraki N., Sedaghati M., Reduction of Biogenic Amines in Fermented Fish Sauces by Using Lactic Acid Bacteria, J. Survey in Fisheries Sci, 5(2): 99-110 (2019).
[37] Nguyen A.,  Sekar A.,   Kim M.,  Nguyen L.,  Le N., Uh S.,  Hong S., Kim K., Fish Sauce Fermentation Using Marinococcus halotolerans SPQ Isolate as a starter culture. Food Sci. and Nutr. 9(2):651-661 (2020).
[39] Onyeike E.N., Ayalogu E.O., Ibegbulem C.O., Evaluation of the Nutritional Value of Some Crude Oil Polluted Freshwater Fishes, Glob. J. Pure Applied Sci., 6: 227-233 (2000).
[40] Park J.N.,  Fukumoto Y.,  Fujita E., Tanaka T., Washio T., Otsuka S., Shimizu T., Watanabe K. Abe H.,  Chemical Composition of Fish Sauces Produced in Southeast and East Asian Countries, J. Food Composition and Analysis., (14): 113-125 (2001).
[41] Park J. Surimi & Surimi Sea Food, Taylor & Francis Group. Second Edition. New York, USA (2005).
[45] Ranjbar M., Mazloomi S.M., Armin M., Hemmati F., Microbial and Chemical Properties of Mahyaveh: A traditional Iranian Fish Sauce in Zarrin Dasht City, Iran, Int. J. Nutr Sci., 2(4): 229-233 (2017).
[46] Saisithi P., Kasemsarn R.O., Liston J., Dollar A., Microbiology and Chemistry of Fermented Fish, J. Food Sci., 31(1):105-110 (2006).
[47] Sedaghati M., Nazari M., Mooraki M., Chemical and Microbial Properties of a Fermented Fish Sauce in the Presence of Lactobacillus Plantarum and Paenibacillus Polymyxa, Iran. J. Fisheries Sci., 20(3):663-667 (2021).
[48] Siringan P., Raksakulthai N., Yongsawatdigul J., Autolytic activity and Biochemical Characteristics of Endogenous Proteinases in Indian Anchovy (Stolephorus indicus), Food Chem., 98: 678–84 (2006).
 [49] Simpson B.K., “Digestive Proteinases from Marine animals. In Seafood Enzymes: Utilization and Influence on Postharvest Seafood Quality”, Haard N.F., Simpson B.K., (Editors), Marcel Dekker, New York, pp. 531-540 (2000).
[51] Taheri A., Jalali Nejad S., Hosseini V., Ahmadi A., Naseri F.,  Survey of the Population of Bacteria in Iranian Fish Sauce (Mahyaveh). Res J. Shahid Beheshti University of Medical Sci., 5: 273-280 (2014).
[52] Takano T., Shozen K., Satomi M., Taira W., Abe H., Funatsu Y., Quality of Fish Sauce Products from Recycled by-Products from Fish Gel and Kamboko Processing, J. Food Quality, 35: 217- 227 (2012).
[53] Thuy P.V., Berger J., Nakanishi Y., Khan N.C., Lynch S., Dixon P., The use of Na Fe Childbearing Age in Rural Vietnam. J. Nutr., (135): 2596-2601 (2005).
[54] Tsiae Y., Lin C.Y.,  Chien L.T.,  Lee T.M., Wei C.­I.,  Hwang D.F., Histamine Contents of Fermented Fish Products in Taiwan and Isolation of Histamine-Forming Bacteria, Food Chem, (98): 664-670 (2006).
[55] Vioque J., Clemente A., Pedroche J., Yust M., Millgn F., Obtenction Yaplicaciones De Hydrolize Proteicos, J. Grasas Aceites., (52):132-136 (2001).
[56] Yao X., Zhang D., Zu Y., Fu Y., Luo M., Gu C., Li C.­M.­F., Efferth T. Free Radical Scavenging Capability, Antioxidant Activity, and Chemical Constituents of Pyrola Incarnata Fisch Leaves, Indust Crops and Product., 49: 247– 255 (2013).
[57] Yang S., He Y., Yan Y., Xie N., Song Y., Yan X., Textural Properties of Stinky Mandarin Fish (Siniperca chuatsi) During Fermentation Effects of the State of Moisture, Int. J. Food Prop., 20: 1530–1538 (2017).
[58] Yin L.J., Pan C.­L., Jiang S.­T.,  Effect of Lactic Acid Bacterial Fermentation on the Characteristics of Minced Mackerel, J. Food Sci., 67(2): 786-792 (2006).
[59] Yazdanpanah P., Mahasti P., Evaluation of the Nutritional Value of Sardine Sauce, Scientific Res J. Food Sci and Technol, third year, 4. (2011).
[60] Zarei M., Najafzadeh H., Eskandari M.H., Pashmforoush M., Enayati A., Gharibi D.,  Chemical and Microbial Properties of Mahyaveh, a Traditional Iranian Fish Sauce, Food Control., 2(23):  511-514 (2012).