Evaluation, Preparation, and Characterization of Chitosan/ZnO Nanocomposite and Antibacterial Activity Against Pathogenic Microbial Strains

Document Type : Research Article


1 Student Research Committee, Kerman University of Medical Sciences, Kerman, I.R. IRAN

2 Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, I.R. IRAN

3 Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, I.R. IRAN


Objective (s): Background: Nanotechnology is used as a tool to develop advanced therapies and control the fight against infections. The aim of this study was to evaluate the physicochemical properties such as morphological analysis of the chitosan nanocomposite on oxide composite through a simple method and to investigate their anti-bacterial properties of them. Materials and Methods: The study method in this study was experimental and the chitosan-zinc oxide nanocomposite was chemically precipitated after preparation of the Chitosan/ZnO nanocomposite physicochemical properties and antibacterial activity against pathogenic microbial strains were investigated. The nanocomposite was evaluated using SEM, FT-IR techniques, XRD X-ray diffraction, and DLS particle size distribution. The antimicrobial effect of this nanocomposite was evaluated on the bacteria Staphylococcus aureus and Micrococcus luteus. In this study, the antimicrobial effect of Chitosan/ZnO nanocomposite ZnO nanoparticles loaded in chitosan was investigated by MIC method on microorganisms (Candida albicans, Microscotus luteus, and Staphylococcus aureus). Results: The results showed that the concentration of zinc oxide nanoparticles affected the antimicrobial activity of chitosan nanocomposite. In this study, the antimicrobial behavior of the Chitosan/ZnO nanocomposite zinc oxide was determined against pathogenic microbial strains of bacteria including Escherichia coli, Pseudomonas aeruginosa, Serratia marcescens, Micrococcus luteus, Bacillus subtilis, Staphylococcus aureus, and Klebsiella pneumoniaagainst E.Coli was investigated and the results show that zinc oxide has an antimicrobial effect against Escherichia coli and also the use of two types of dispersants (peg/pvp) on antimicrobial activity of zinc with MIC (Minimum Inhibitory Concentration) approach. The ZnO nanoparticles oxide has no effect and only the Chitosan/ZnO nanocomposite increases the stability of the suspensions. SEM analysis shows that synergistic effect on the destruction of the bacterial wall. The nanoparticles on the oxide damage the bacterial wall. Conclusion: Based on the results, the synthesized compounds have an antimicrobial effect and the antimicrobial effect has increased with increasing polymer (chitosan) concentration. The antimicrobial effect has been seen on gram-positive and gram-negative bacteria.


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