Liquid - Liquid Extraction of 2, 3-Butanediol from Fermentation Broth

Document Type: Research Article

Authors

1 Faculty of Chemical Engineering, Tarbiat Modares University, P.O. Box 14115-143 Tehran, I.R. IRAN

2 Department of Chemical Engineering, Engineering Faculty, Guilan University, P.O. Box 41635-3756 Rasht, I.R. IRAN

3 Department of Biotechnology, College of Science, Tehran University P.O. Box 14155-6455 Tehran, I.R. IRAN

Abstract

Recovery of metabolites from fermentation broth by solvent extraction can be used to optimize fermentation processes. End-product reutilization, low product concentration, large volumes of fermentation broth and the requirements for large bioreactors, in addition to the high cost largely contributed to the decline in fermentative 2, 3-butanediol production. Extraction can successfully be used for in-situ alcohol recovery in 2, 3-butanediol fermentations to increase the substrate conversion.In the present work organic extraction of 2, 3-butanediol produced by Klebsiella pneumoniae fermentation was studied to detect solvent effect on 2, 3-butanediol production and determination of efficient volume of solvent. The aim of this research was liquid-liquid extractive fermentation systems evaluation as an alternative to overcome the end product effect and to increase of 2, 3-butanediol production by K. pneumoniae because conventional fermentative production of 2, 3-butanediol by K. pneumoniae hasthe disadvantage of product reutilization by the organism. The highest 2, 3-butanediol production (23.01 g/L) achieved when 20% oleyl alcohol was used.  

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