Bisubstrate Kinetic Model for Enzymatic Decolorization of Reactive Black 5 by Coprinus cinereus Peroxidase

Document Type: Research Article


Department of Chemical and Petroleum Engineering, Sharif University of Technology, P.O. Box 11155-9465 Tehran, I.R. IRAN


In this study, decolorization of the diazo dye, Reactive Black 5 (RB5) in a Coprinus cinereus peroxidase-catalyzed reaction has been investigated. A bisubstate kinetic model for the reaction rate based on the Ping-Pong mechanism was assumed for the enzymatic decolorization. Experimentswere conducted at different RB5 and hydrogen peroxideconcentrations in a batch mannerto estimate the intrinsic kinetic parameters. These parameters were used for the modeling of decolorization in a continuous reactor that was compared with experimental results. An acceptable agreement was observed between the model and experimental data.


Main Subjects

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