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Zakeri, A., Pazouki, M., Vossoughi, M. (2017). Use of Response Surface Methodology Analysis for Xanthan Biopolymer Production by Xanthomonas campestris: Focus on Agitation Rate, Carbon Source, and Temperature. Iranian Journal of Chemistry and Chemical Engineering (IJCCE), 36(1), 173-183.
Ali Zakeri; Mohammad Pazouki; Manouchehr Vossoughi. "Use of Response Surface Methodology Analysis for Xanthan Biopolymer Production by Xanthomonas campestris: Focus on Agitation Rate, Carbon Source, and Temperature". Iranian Journal of Chemistry and Chemical Engineering (IJCCE), 36, 1, 2017, 173-183.
Zakeri, A., Pazouki, M., Vossoughi, M. (2017). 'Use of Response Surface Methodology Analysis for Xanthan Biopolymer Production by Xanthomonas campestris: Focus on Agitation Rate, Carbon Source, and Temperature', Iranian Journal of Chemistry and Chemical Engineering (IJCCE), 36(1), pp. 173-183.
Zakeri, A., Pazouki, M., Vossoughi, M. Use of Response Surface Methodology Analysis for Xanthan Biopolymer Production by Xanthomonas campestris: Focus on Agitation Rate, Carbon Source, and Temperature. Iranian Journal of Chemistry and Chemical Engineering (IJCCE), 2017; 36(1): 173-183.

Use of Response Surface Methodology Analysis for Xanthan Biopolymer Production by Xanthomonas campestris: Focus on Agitation Rate, Carbon Source, and Temperature

Article 17, Volume 36, Issue 1 - Issue Serial Number 81, Winter 2017, Page 173-183  XML PDF (286 K)
Document Type: Research Article
Authors
Ali Zakeri1; Mohammad Pazouki 2; Manouchehr Vossoughi3
1Environmental Group, Energy Department, Material, and Energy Research Center, Karaj, I.R. IRAN
2Institute of Materials and Energy
3Chemical and Petroleum Engineering Department, Sharif University of Technology, Tehran, I.R. IRAN
Abstract
The current study is an attempt to contribute for efficient and cost-effective substrates for xanthan gum production. In this context, the sugar cane molasses wastes can be used as a cheap substrate for xanthan gum production. Xanthan biopolymer production by a novel Xanthomonas campestris strain IBRC-M 10644 was optimized with statistical approaches. Based on the results of Response Surface Methodology (RSM) with Central Composite Design (CCD) technique, a second-order polynomial model was developed and evaluated the effects of variables on the maximum xanthan production. Agitation rate (X1: 200-500 rpm), sugar cane molasses concentration (X2: 30-90 g/L) and operation temperature (X3: 25-35 °C) were the factors investigated. The optimal conditions for maximum yield of xanthan production were derived from agitation rate 500 rpm, carbon source concentration 65 g/L and operation temperature 30°C. Under these conditions, xanthan and biomass production were found to be 16.03 g/L and 1.37 g/L, respectively. Our results signify that the sugar cane molasses can be used as a cheap substrate for xanthan biopolymer production.
Keywords
Xanthan; Sugar cane molasses; Optimization; Central composite design
Main Subjects
Biotechnology; Oil, Gas & Petrochemical Industries
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