TY - JOUR ID - 25206 TI - Use of Response Surface Methodology Analysis for Xanthan Biopolymer Production by Xanthomonas campestris: Focus on Agitation Rate, Carbon Source, and Temperature JO - Iranian Journal of Chemistry and Chemical Engineering JA - IJCCE LA - en SN - 1021-9986 AU - Zakeri, Ali AU - Pazouki, Mohammad AU - Vossoughi, Manouchehr AD - Environmental Group, Energy Department, Material, and Energy Research Center, Karaj, I.R. IRAN AD - Chemical and Petroleum Engineering Department, Sharif University of Technology, Tehran, I.R. IRAN Y1 - 2017 PY - 2017 VL - 36 IS - 1 SP - 173 EP - 183 KW - Xanthan KW - Sugar cane molasses KW - Optimization KW - Central composite design DO - 10.30492/ijcce.2017.25206 N2 - The current study is an attempt to contribute for efficient and cost-effective substrates for xanthan gum production. In this context, the sugar cane molasses wastes can be used as a cheap substrate for xanthan gum production. Xanthan biopolymer production by a novel Xanthomonas campestris strain IBRC-M 10644 was optimized with statistical approaches. Based on the results of Response Surface Methodology (RSM) with Central Composite Design (CCD) technique, a second-order polynomial model was developed and evaluated the effects of variables on the maximum xanthan production. Agitation rate (X1: 200-500 rpm), sugar cane molasses concentration (X2: 30-90 g/L) and operation temperature (X3: 25-35 °C) were the factors investigated. The optimal conditions for maximum yield of xanthan production were derived from agitation rate 500 rpm, carbon source concentration 65 g/L and operation temperature 30°C. Under these conditions, xanthan and biomass production were found to be 16.03 g/L and 1.37 g/L, respectively. Our results signify that the sugar cane molasses can be used as a cheap substrate for xanthan biopolymer production. UR - https://ijcce.ac.ir/article_25206.html L1 - https://ijcce.ac.ir/article_25206_852e7564621adf861086bc768ebfd46f.pdf ER -