Separation of Sedative – Hypnotic Drugs with Mixed Micellar Liquid Chromatography

Document Type : Research Article


Department of Chemistry, University of Mazandaran , P. O. Box 453, Babolsar, I. R. IRAN


Separation of ten sedative- hypnotic drugs was performed by RP-HPLC using mixed micellar mobile phase. Effect of temperature, type and amount of organic modifier in mobile phase on efficiency (N) and asymmetry factor (B/A) showed that, the appropriate conditions for a good separation were 35°C and 7% (V/V) butanol in mobile phase.  Variations of selectivity factor versus butanol concentration, Mixture of SDS/ Brij- 35 concentrations and pH of mobile phase showed that the appropriate conditions were 7%(V/V), 15 mM SDS +1.0mM Brij-35 and pH=3 in mobile phase respectively.


Main Subjects

[1] Woods, J.H., Katz, J. L. and Winger, G., Pharmacol. Rev., 39, 254, (1987).
[2] Owen, R. T. and Tyrer, P., Drugs., 25, 385, (1983).
[3] Drummer, O. H. and Ranson, D. L., Am. J. Forensic Med. Pathol., 17, 336, (1996).
[4] Worm, K., Steentoft, A. and Toft, J., J. Traffic Med., 24, 39, (1996).
[5] Katzung, B. G., “Basic & Clinical Pharmacology”, Sixth Ed. Appleton & lange., USA, (1995).
[6] Worm, K., Steentoft, A. and Christensen, H., Traffic Med., 19, 3 (1991).
[7] Drummer, O. H. and Ranson, D. L., Am. J. Forensic Med. Pathol., 17, 336, (1996).
[8] Mcintyre,  I.  M., Syrjanen, M. L., Grump, K., Horomidis, S., Peace,. A.W. and Drummer, O. H., J. Anal. Toxicol., 17, 202, (1993).
[9] Ferrara, S.D., Tedeschi, L., Frison, G. and Castagna, F., J. Anal. Toxicol., 16, 217, (1992).
[10] Huang, W. and Moddy, D. E., J. Anal. Toxicol., 19, 333, (1995).
[11] Hadjmohammadi, M.R. and Ebrahimi, P., Inter-national J. Chem., 9, 101, (1999).
[12] Arunyanart, M. and Cline Love, L. J., Anal. Chem., 56, 1557 (1984).
[13] Horvath, C. and Melander W. R., Anal. Chem., 49, 2295, (1977).
[14] Brogerding, M. F.,  Quina, F. H.,  Hinze, W. L., Bowermaster, J. and Mcnair, H. M., Anal. Chem., 60, 2520, (1988).
[15] Brogerding, M. F. and Hinze. W. L., Anal. Chem., 61, 1353, (1989).
[16] Haginaka, J., Wakai, J. and Yasuda. H., J. Chromatogr, 480, 341, (1989).