Determination of Juglone (5-hydroxy 1,4-naphthoquinone) in Pterocarya fraxinifolia by RP-HPLC

Document Type : Research Article


Department of Chemistry, University of Mazandaran, P.O.Box 453 Babolsar, I.R. IRAN


A method for determination of juglone in leaves and hulls of Pterocarya fraxinifolia was developed, based on RP-HPLC. This compound was extracted from leaves and hulls by chloroform refluxing about 2 hours. The chloroform was evaporated under low pressure at   50 ºC to produce a dry residue. The remained materials were dissolved in acetonitrile to be used for determination of this compound. Appropriate conditions for RP-HPLC were determined using standard of juglone. These conditions include, acetonitrile percentage in mobile phase, pH of mobile phase (phosphate buffer) and temperature of column, which were 50%, 4 and 30ºC, respectively. Juglone concentration was determined using standard addition method. The concentrations of juglone were 2.15, 2.74,1.77,1.12and 0.34 g in 100 g of dry leaves in May, June, July, August and September 2001, respectively. The content of juglone in hulls was 0.44 g in 100 g of dry hulls in May.


Main Subjects

[1] Botanical Dermatology Database, 1999, Juglandaceae, [].
[2] Parsa, A., “Flore d’ Iran” (Imprimere Mazaheri Tehran,) No. VI, 1316, (1979).
[3] Kruz, T., Stossel, S., Spiller A., US Patent No.5569460, (1996).
[4] Aynechi, Y.,  Dehpour, A.R, Phytochemistry, 12, 3001 (1973).
[5] Thamson, R.H, “NaturallyOccurring Quinone”, Academic press, Section 2, 360, (1971). 
[6] Kulkari, B.A., Kelker, V.D., Kukarni, P.L., Indian J. Pharm.Sci., 45, 21 (1983).
[7] Bobich, H., Stern, A., J. Appl. Toxicol, 13 (5), 353 (1993).
[8] Wojcik, E., Farm.Pol., 40, 523 (1984).
[9] Lemberkovics, E., Hethelyi, E., Hetesi, E., Acta Pharm.Hung., 57, 133, (1987).
[10] Van Damme, J.G., De Nave E., J. Pharm. Sci., 68, 16 (1979).
[11] Stensen, W., Jensen E., J. of Chromatogr. A, 659, 87, (1994).
[12] Welcher, F. J., ”Standard  Methods  of Chemical Analysis”, R.E. Krieger Publishing Company, part two, 1249 (1975).