Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer

Document Type : Research Article

Authors

1 Department of Chemical Engineering, Amirkabir University of Technology, P.O. Box 15874-4413 Tehran, I.R. IRAN

2 Department of Biomedical Engineering, Amirkabir University of Technology, P.O. Box 15875-4413 Tehran, I.R. IRAN

Abstract

In this research, an enzyme modified electrode has been produced during the electropolymerization of aniline through incorporation of sulfite oxidase into a conducting polymer. Then the bioelectrochemical response of resulted sulfite biosensor was investigated at different experimental conditions. Study of  the stability of the resulted sulfite biosensor revealed that formation of a passive film on the aluminum surface causes improved stability of the electroactive films formed on the electrode surface. The bioelectrochemical response of the enzyme-modified electrode as a sulfite biosensor was investigated at different experimental conditions. The optimum pH and temperature were 8.5 and 35°C, respectively. The apparent Michaelis-Menten constant and the activation energy of the enzyme catalyzed reaction were calculated.

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References

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