Intensive Bioethanol Production Using Date Stems By-products and Natural Sugars

Document Type : Research Article

Authors

Laboratoire Phénomènes de transfert (LPDT),Université des Sciences et de la Technologie Houari Boumediene (USTHB), Bab-Ezzouar, Algiers, ALGERIA

Abstract

Date Stem By-products (DSB) have been used as agricultural feedstock to perform bioethanol production in a batch bioreactor. To study the effect of an optimal mixture of fruit hydrolysates (20%dates, 20% Figs, and 20% sugar beet) on ethanol yield, different parameters have been followed up, including pH, total sugars, and ethanol yield. The optimal ethanol concentration was found for date stems by-products +20% dates, it was about 19.38 g/L after 72h fermentation process, in such case, the higher initial sugar concentration is close to 204.5 g/L, and the pH dropped from 4.5 to 4.21, while it was only about 18.48, 13.08 and 4.72 g/L for DSB+20% sugar beet, DSB+20% Figs, and DSB, respectively. The maximum bioethanol production rate (Rm) was about 2.633 (g/L/h) in the case of DSB+20% dates, which is higher compared to the other selected mixtures. The effect of some physical properties on the addition of ethanol/ETBE (Ethyl tertiary-butyl ether) to super-premium gasoline has been studied. It was found that the addition of 20% ethanol or ETBE to super-premium gasoline increased the Research Octane Number from 96 up to 99.3 and from 96 up to 99.8 in the case of DSB+20% dates and ETBE, respectively.

Keywords

Main Subjects


[1] Zentou H., Rosli, N.S., Wen C.H., Abdul Azeez, K., Gomes C., The Viability of Biofuels in Developing Countries: Successes, Failures, and ChallengesIranian Journal of Chemistry and Chemical Engineering (IJCCE)38(4): 173-182 (2019).
[2] Panchuk M., Kryshtopa S., Sładkowski A., Panchuk A., Environmental Aspects of the Production and Use of Biofuels in Transport, In “Ecology in Transport: Problems and Solutions”., 115-168. Springer, Cham. (2020).
[3] Baeyens J., Kang Q., Appels L., Dewil R., Lv Y., Tan T., Challenges and Opportunities in Improving the Production of Bio-Ethanol, Progress in Energy and Combustion Science, 47: 60–88 (2015).
[4] Louhichi B., Belgaib J., Benamor H., Hajji N., Production of Bio-Ethanol from three Varieties of Dates, Renewable Energy, 51: 170-174 (2013).
[5] Barros S., "Biofuels Annual Brazil" (2017).
[6] Keskin A., Yaşar A., Reşitoğlu  İ., M. A., Sugözü İ., The Influence of Diesel Fuel-biodiesel-ethanol-butanol Blends on the Performance and Emission Characteristics of a Diesel Engine. Energy Sources, Part A: Recovery, Utilization, and Environmental Effects, 35: 1873-1881 (2013).
[7] Azadi H., Jong S., Reudder B.,  Maeyer P., Witlox F., How Sustainable is Bio-Ethanol Production in Brazil?, Renewable and Sustainable Energy Reviews, 16: 3599-3603 (2012).
[8] Gumienna M., Szwengiel A., Lasik M., Szambelan K., Majchrzycki D., Adamczyk J.,  Nowaka J., Czarnecki Z., Effect of Corn Grain Variety on the Bioethanol Production Efficiency, Fuel, 164: 386-392 (2016).
[9] Soares J.,  Demeke M.M., Foulquié-Moreno M.R., Van de Velde M., Verplaetse A.,  Fernandes A.A.R.,  Thevelein J.M., Fernandes P.M.B., Green Coconut Mesocarp Pretreated by an Alkaline Process as Raw Material for Bioethanol Production, Bioresource Technology, 216: 744-753 (2016).
[10] Derman E., Abdulla R., Marbawi H.,  Sabullah M.K., Oil Palm Empty Fruit Bunches as a Promising Feedstock for Bioethanol Production in Malaysia, Renewable Energy, 129: 285-298 (2018).
[11]Song Y., Cho E.J.,  Park C.S.,  Oh C.H., Bok-Jae Park B.J, Bae H.J., A Strategy for Sequential Fermentation by Saccharomyces Cerevisiae and Pichia Stipitis in Bioethanol Production from Hardwoods, Renewable Energy, 139: 1281-1289 (2019).
[13]  Horn S.J., I.M. Aasen, Ostgaard K.., Ethanol Production from Seaweed Extract, Journal of Industrial Microbiology and Biotechnology, 25: 249–254 (2000).
[14] FAO, "Production Yearbook". (2019).
[15] Pan X., Arato C., Gilkes N., Gregg D., Mabee W., Pye K., Xiao Z., Zhang X., Saddler J., Biorefining of Softwoods Using Ethanol Organosolv Pulping: Preliminary Evaluation of Process Streams for Manufacture of Fuel-Grade Ethanol and Co-Products, Biotechnology and Bioengineering, 90: 473–81 (2005).
[16]  Cabaret F., Fradette L., Tanguy P.A., Gas-Liquid Mass Transfer in Unbaffled Dual-Impeller Mixers, Chemical Engineering Science, 63: 1636–1647 (2008).
[17] Williams J.A., Keys to Bioreactor Selection, Chemical Engineering Progress, 98: 34–41 (2002).
[18] Sluiter A., Hames B., Ruiz R., Scarlata C., Sluiter J., Templeton D., Crocker D. L. A. P., Determination of Structural Carbohydrates and Lignin in Biomass, Laboratory Analytical Procedure, 1617 (1): 1-16 (2008).
[19]  Sluiter A., Hames B., Ruiz R., Scarlata C., Sluiter J., Templeton D., Crocker D., Determination of Structural Carbohydrates and Lignin in Biomass, NREL Chemical Analysis and Testing Laboratory Analytical Procedures:; LAP-002, NREL/TP-510-42618 (2012).
[20] Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A., Smith F., Colorimetric Method for Determination of Sugars and Related Substances. Analytical Chemistry 28 (3): 350-356 (1956).
[21] Lowry O.H., Rosebrough N.J., Farr A.L., Randall R.J., Protein Measurements with the Folin Phenol Reagent. Journal of Biological Chemistry, 193(1): 265–275 (1951).
[22] Bligh E.G., Dyer W.J., A Rapid Method of Total Lipid Extraction and Purification1. Can. J. Biochem. Physiol., 37: 911- 917 (1959).
[23] Chohan N.A., Aruwajoye G.S., Sewsynker-Sukai Y.,  Kana E.G., Valorisation of Potato Peel Wastes for Bioethanol Production Using Simultaneous Saccharification and Fermentation: Process Optimization and Kinetic AssessmentRenewable Energy,  146: 1031-1040 (2019).
[24] Zerrouki S., Rihani R., Lekikot K., Ramdhane I., Enhanced Biogas Production from Anaerobic Digestion of Wastewater from the Fruit Juice Industry by Sonolysis: Experiments and Modelling, Water Science and Technology, 84(3): 644-655 (2021).
[25] Akin H., Evolution du pH Pendant la Fermentation Alcoolique de Moûts de Raisins : Modélisationet Interprétation Métabolique , Doctoral Dissertation, Institue National Polytechnique Toulouse (2008).
[26] Carmelo V., Bogaerts P., SáCorreia I., Activity of Plasma Membrane H+-ATPase and Expression of PMA1 and PMA2 Genes in Saccharomyces cerevisiae Cells Grown at Optimal and Low pH, Archives of Microbiology, 166 (5): 315–20 (1996).
[28] Dickinson JR., Schweizer M., “The Metabolism and Molecular Physiology of Saccharomyces Cerevisiae”. 2nd ed. USA: CRC Press (2004).
[29] Chaira N, Mrabet A, Ferchichi A., Evaluation of Antioxidant Activity, Phenolics, Sugar and Mineral Contents in Date Palm Fruits. Journal of Food Biochemistry 33: 390-403 (2009).
[30] Karagöz P., Rocha I.V., Özkan M., Angelidaki I., Alkaline Peroxide Pretreatment of Rapeseed Straw for Enhancing Bioethanol Production by Same Vessel Saccharification and Co-FermentationBioresource Technology104: 349-357 (2012).
[32] Rodríguez-Antón L.M., Gutiérrez-Martín F., Martinez-Arevalo. C., Experimental Determination of Some Physical Properties of Gasoline, Ethanol and ETBE Ternary Blends, Fuel, 156: 81–86 (2015).
[33] Hsieh W.-D., Chen R.-H., Wu T.-L., Lin T.-H., Engine Performance and Pollutant Emission of an SI Engine Using Ethanol–Gasoline Blended Fuels, Atmospheric Environment, 36(3): 403-410 (2002).
[35] Stan Jones D.S.J.., Pujadó P.R., “  , Springer (2006).
[36] MRiazi R., Nasimi N., Roomi Y., Estimating Sulfur Content of Petroleum Products and Crude Oils, Industrial & Engineering Chemistry Research, 38: 4507-4512 (1999).
[37] Al-Baghdadi M.A.R.S., Measurement and Prediction Study of the Effect of Ethanol Blending on the Performance and Pollutants Emission of a Four-Stroke Spark Ignition Engine, Proceedings of the Institution of Mechanical Engineers, Part D: Journal of Automobile Engineering, 222(5): 859-873 (2008).
[38] Fahim M.A., Al-Sahhaf T.A., Elkilani A.., “Fundamentals of Petroleum Refining”, Elsevier Science (2009).