The Study on Microbial Polymers: Pullulan and PHB

Document Type : Research Article


Department of Chemical and Petroleum Engineering, Sharif University of Technology, P.O. Box 11155-9465 Tehran, I.R. IRAN


Microbial cells are producers of natural polymers present in plant cells. Production of pullulan (an extracellular microbial polysaccharide) by Aureobasidium pullularia pullulans (P. pullulans) was studied under fermentation conditions, and kinetic parameters were determined. Pullulan formation obeyed a growth and non-growth associated term. PHB (polyhydroxybutyrates) an intracellular biopolymer production by Rastonia eutropha (Alcaligen eutrophus), R. eutropha was studied under different culture media, including synthetic and natural carbon sources. Molasses as a natural carbon source in the culture media presented high efficiency in cell and biopolymer accumulation.


Main Subjects

[1] Moo-Young, M., “Comprehensive Biotechnology”, Microbial Polysaccharide, vol 3, Pergamon Press INC, London, UK, pp. 1005-1015 (1985).
[2] Alemzadeh, I., Vossoughi, M. and Naimpour, F., Biopolymer Production and Kinetics, International Seminar   of   Polymer   Science   and    Technology, pp. 449- 455, Shiraz, I.R. IRAN (1994).
[3] Alemzadeh, I., Vossoughi M. and Fan, L.T., A Study on Fructose Production from Fermentation Media with High Sucrose Concentration by P. pullulans, Chisa Proceeding, Praha Chec, September (1994).
[4] Li, H., Chi, Z., Wang, X., Duan, X., Ma, L., Gao, L., Purification and Characterization of Extracellular Amylase from the Marine Yeast Aureobasidium Pullulans N13d and its Raw Potato Starch Digestion, Enzyme and Microbial Technology, 40, p. 1006 (2007).
[5] Leite, R.S.R., Gomes, E., da Silva, R., Charac-terization and Comparison of Thermostability of Purified ß-Glucosidases from a Mesophilic Aureobasidium Pullulans and a Thermophilic Thermoascus aurantiacus, Process Biochemstry, 42, p. 1101 (2007).
[6] De Wet, B.J.M., Van Zyl, W. H., Prior, B. A., Characterization of the Aureobasidium Pullulans
α-Glucuronidase Expressed in Saccharomyces Cerevisiae, Enzyme and Microbial Technology, 38, p. 649 (2006).
[7] Sangeetha, P.T., Ramesh, M.N., Prapulla, S.G., Production of Fructo-Oligosaccharide by Fructosyl Transferase from Aspergillus Oryzae CFR 202 and Aureobasidium Pullulans CFR 77, Process Biochemistry, 39, p. 753 (2004).
[8] Duan, X., Chi, Z., Wang, L., Wang, X., Influence of Different Sugars on Pullulan Production and Activities of α-Phosphoglucose Mutase, UDPG-Pyrophosphorylase and Glucosyltransferase Involved in Pullulan Synthesis in Aureobasidium Pullulans Y68, Carbohydrate Polymers, 73, p. 587 (2008).
[9] Zheng, W., Campbell, B. S., McDougall, B. M., Seviour, R.J., Effects of Melanin on the Accumulation of Exopolysaccharides by Aureobasidium Pullulans Grown on Nitrate, Bioresource Technology, 99, p. 7480 (2008)
[10] Singh, R. S., Saini, G. K., Kennedy, J. F., Pullulan: Microbial Sources, Production and Applications, Carbohydrate Polymers, 73, p. 515 (2008).
[11] Souguir, Z., Roudesli, S., Picton, E. L., Le Cerf, D., About-Jaudet, E., Novel Cationic and Amphiphilic Pullulan Derivatives I: Synthesis and Characterization, European Polymer Journal, 43, p. 4940 (2007).
[12] Seidi, A. and Alemzadeh, I., Poly Hydroxyl Butyrate Production, 8th Chemical Engineering Congress,
pp. 115-120, Mashad, I.R. IRAN (2003).
[13] Seung yoo, Woo-Sik kim, Cybemetic Model for Synthesis of Poly B-Hydroxy Butyric Acid in Alcaligenes Eutrophus, Biotechnology and Bio-engineering, 43, p. 1043 (1994).
[14] Brandi, H., Gross, R.A., Lenz, R.W. and Clinton, R., Plastics from Bacteria: Polyhydroxyalkanoates
as Natural, Biocompatible, and Biodegradable Polyesters, Advances in Biochemical Engineering, 41, p. 77 (1990).
[15] Katircloglu, H., Aslim, B., Nuryuksek, D., Mercan, N. and Beyatli, Y., Production of Poly-B-Hydroxbutyrate (PHB) and Differentiation of Putative Bacillus Mutant Strains by SDS-PAGE of Total Cell Protein, African Journal of Biotechnology, 2(6), p. 147 (2003).
[16] Aslim,  B.,  Nuryuksek,  D.  and  Beyatli,  Y., Determination of PHB Growth Quantities of Certain Bacillus Species Isolated from Soil, Turkish Electronic Journal of Biotechnology, Special Issue, p. 24 (2002).
[17] Linko, S., Vaheri, H. and Seppala, J., Production of Poly-hydroxybutyrate on Lactic Acid by A. eutrophus H16 in a 3-l Bioreactor, Enzyme and  Microbial Technol., 15, p. 401 (1993).
[18] Kim, B.S., Lee, S.C., Chang, H.N. and Woo, Y.K., Production of Poly3- Hydroxy Butyrate Acid by Fed-Batch Culture of Recombinant E.coli, Biotechnology. Letters, 4, p. 811 (1992).
[19] Jung, L., Phyo, K.H., Kim, K.C., Park, H.K., Kim, I.G., Spontaneous Liberation of Intracellular Polyhydroxy-Butyrate Granules in Escherchia coli, Research in Microbilogy, 156, p. 865 (2005).
[20] Valappil,  S. P., Misra,  S. K.,  Boccaccini,  A. R., Keshavarz, T., Bucke, C., Roy, I., Large-Scale Production and Efficient Recovery of PHB with Desirable Material Properties, from the Newly Characterized Bacillus Cereus SPV, J. of Bio-technology, 132, p. 251 (2007).
[21] Monteil-Rivera,  F.,  Betancourt,  A.,  Tra,  H.V., Yezza, A., Hawari, J., Use of Headspace Solid-Phase Micro Extraction for the Quantification of Poly(3-Hydroxybutyrate) in Microbial Cells, J. of Chromatography A, 1154, p. 34 (2007).
[22] Theodorou, M.C., Panagiotidis, C.A., Panagiotidis, C.H., Pantazaki, A.A., Kyriakidis, D.A., Involvement of the AtoS-AtoC Signal Transduction System in Poly-(R)-3-Hydroxybutyrate Biosynthesis in Escherchia Coli, Biochimica et Biophysica Acta, 1760, p. 896 (2006).
[23] Ganduri V.S.R.K., Ghosh, S., Patnaik, P.R., Mixing Control as a Device to Increase PHB Production in Batch Fermentations with Co-Cultures of Lactobacillus Delbrueckii and Ralstonia Eutropha, Process Biochemistry, 40, p. 257 (2005).
[24] Taguchi, S., Maehara, A., Takase, K., Nakahara, M., Nakamura, H., Doi, Y., Analysis of Mutational Effects of a Polyhydroxybutyrate (PHB) Polymerase on Bacterial PHB Accumulation Using an in Vivo Assay System, FEMS Microbiology Letters, 198, p. 65 (2001).
[25] Saad, G.R., Seliger, H., Biodegradable Copolymers Based on Bacterial Poly((R)-3-Hydroxybutyrate): Thermal and Mechanical Properties and Bio-degradation Behavior, Polymer Degradation and Stability, 83, p. 101. (2004).
[26] Song, B.G., Kim, T.K., Jung, Y.M., Lee, Y.H., Modulation of talA Gene in Pantose Phosphate Pathway for Overproduction of Poly-ß-Hydroxy-butyrate in Transformant Escherchia Coli Harboring phbCAB Operon, J. of Bioscience and Bio-engineering, 102, p. 237 (2006).
[27] Wu, G., Moir, A.J.G., Sawers, G., Hill, S., Poole, R.K., Biosynthesis of Poly-ß-hydroxybutyrate (PHB) is Controlled by CydR (Fnr) in the Obligate Aerobe Azotobacter Vinelandi, FEMS Microbiology Letters, 194, p. 215 (2001).
[28] Takanashi, M., Saito, T., Characterization of Two 3-Hydroxybutyrate Dehydrogenases in Poly(3-Hydroxybutyrate)-Degradable Bacterium Ralstonia Pickettii T1, J. of Bioscience and Bioengineering, 101, p. 501 (2006).
[29] Shih, W.J., Chen, Y.H., Shih, C.J., Hon, M.H., Wang, M.C., Structural and Morphological Studies on Poly(3-Hydroxybutyrate Acid) (PHB)/Chitosan Drug Releasing Microspheres Prepared by Both Single and Double Emulsion Processes, J. of Alloys and Compouds, 434-435, p. 826 (2007).
[30] Khosravi  Darani  K.,  Vasheghani  Farahani,  E., Microorganisms   and   Systems   for  Production   of   Poly(Hydroxybutyrate) az an Biodegradable Polymer, Iran J. of Chem. Chem. Eng., 24, 1 (2005).
[31] Khosravi  Darani  K.,  Vasheghani  Farahani,  E., Shojaosadati, S.A., Application of the Plackett-Burmann Design for the Optimization of Poly (ß-Hydroxybutyrate) Production by Ralstonia Eutropha, Iran. J. Biotenchnology, 1, 155 (2003).
[32] Sangkharak,  K.,  Prasertsan,  P.,  Optimization  of Polyhydroxybutyrate Production from a Wild Type and Two Mutant Strains of Rhodobacter Sphaeroides Using Statistical Method, J. of Biotechnology, 132, p. 331 (2007).