Bisubstrate Kinetic Model for Enzymatic Decolorization of Reactive Black 5 by Coprinus cinereus Peroxidase

Document Type : Research Article


Department of Chemical and Petroleum Engineering, Sharif University of Technology, P.O. Box 11155-9465 Tehran, I.R. IRAN


In this study, decolorization of the diazo dye, Reactive Black 5 (RB5) in a Coprinus cinereus peroxidase-catalyzed reaction has been investigated. A bisubstate kinetic model for the reaction rate based on the Ping-Pong mechanism was assumed for the enzymatic decolorization. Experimentswere conducted at different RB5 and hydrogen peroxideconcentrations in a batch mannerto estimate the intrinsic kinetic parameters. These parameters were used for the modeling of decolorization in a continuous reactor that was compared with experimental results. An acceptable agreement was observed between the model and experimental data.


Main Subjects

[1] Meyer U., Biodegradation of Synthetic Organic Colorants. Microbial Degradation of Xenobiotic and Recalcitrant compounds, in: T. Leisinger, A.M. Cook, R. Hunter, J. Nuesch (Eds.), "FEMS Symposium 12", Academic Press, London, p. 371 (1981).
[2] Zollinger H., “Colour Chemistry-Synthesis, Properties of Organic Dyes and Pigments”. VCH Publishers, pp. 92-100 (1987).
[3] Levin L., Papinutti L., Forchiassin F., Evaluation of Argentinean white Rot Fungi for Their Ability to Produce Lignin-Modifying Enzymes and Decolorize Industrial Dyes, J. of Biores. Tech., 94, p. 169 (2004).
[4] Vaidya A.A., Datye K.V., Environmental Pollution During Chemical Processing of Synthetic Fibers, Colourage, 3,p. 14 (1982).
[5] Gon C.¸ Alves I., Gomes A., Bra´s R., Ferra M.I.A., Amorim M.T.P., Porter R.S., Biological Treatment of Effluent Containing Textile Dyes, J. Soc. Dyers and Colourists, 116, p. 393 (2000).
[6] Joshi M., Bansal R., Purwar R., Colour Removal from Textile Effluents, Ind. J Fibre Textile Res., 29, p. 239 (2004).
[7] Mishra G., Tripathy M., A Critical Review of the Treatments for Decolourization of Textile Effluent, Colourage, 40, p. 35 (1993).
[8] Robinson T., McMullan G., Marchant R., Nigam P., Remediation of Dyes in Textile Effluents: a Critical Review on Current Treatment Technologies with a Proposed Alternative, Biores. Technol., 77, p. 247 (2001).
[9] Hai F.I., Yamamoto K., Fukush, K., Hybrid Treatment Systems for Dye Waste waters, Crit. Rev. Environ. Sci. Technol., 37, p. 315 (2007).
[10] Lopez C., Moreira M.T., Feijoo G., Lema J.M., Dye Decolorization by Manganese Peroxidase in An Enzymatic Membrane Bioreactor, Biotechnol. Prog., 20, p. 74 (2004).
[11] Husain Q., Potential Applications of the Oxidoreductive Enzymes in the Decolorization and Detoxification of Textile and Other Synthetic Dyes from Polluted Water: A Review, Crit. Rev. Biotechnol., 60, p. 201 (2006).
[12] Karimi A., Vahabzadeh F., Mohseni M., Mehranian M., Decolorization of Maxilon-Red by Kissiris Immobilized Phanerochaete Chrysosporium in a Trickle-Bed Bioreactor-Involvement of Ligninolytic Enzymes, Iran. J. Chem. Chem. Eng., 28(2), p.1, (2009).
[13] Akhtar S., Husain Q., Potential of Immobilized Bitter Gourd (Momordica charantia) Peroxidase in the Removal of Phenols from Polluted Water, Chemosphere, 65, p. 1228 (2006).
[14] Kariminiaae-Hamedaani H.-R., Sakurai A., Sakakibara M., Decolorization of Synthetic Dyes by a New Manganese Peroxidase-Producing White Rot Fungus, Dyes and Pigments, 72, p. 157 (2007).
[15] Husain Q., Jan U., Detoxification of Phenols and Aromatic Amines from Polluted Waste water by Using Phenol Oxidases, A review, J. Sci. Ind. Res., 59, p. 286 (2000).
[16] Husain Q., Husain M., Kulshrestha Y., Remediation and Treatment of Organopollutants Mediated by Peroxidases: a Review, Crit. Rev. Biotechnol., 29, p. 94 (2009).
[17] Singh H., “Micoremediation - Fungal Bioremediation”, John Wiley and Sons Publication, p. 420 (2006).
[18] Heinfling A., Bergbauer M., Szewzyk U., Biodegradation of azo and Phthalocyanine Dyes by Trametes Versicolor and Bjerkandera Adusta, Appl. Microbiol. Biotechnol., 48, p. 261 (1997).
[19] Alemzadeh I., Nejati S., Removal of Phenols with Encapsulated Horseradish Peroxidase in Calcium Alginate, Iran. J. of Chem. & Chem. Eng., 28(2), p.43, (2009).
[20] Dunford H.B., On the Function and Mechanism of Action of Peroxidases, Coord. Chem. Rev., 19, p. 187 (1976).
[21] Wright H., Nicell J.A., Characterization of Soybean Peroxidase for the Treatment of Aqueous Phenol, Bioresource Technol., 70, p. 69 (1999).
[22] Tong Z., Qingxiang Z., Hui H., Quin L., Yin Z., Kinetic Study on the Removal of Toxic Phenol and Chlorophenol from Waste water by Horseradish Peroxidase, Chemosphere, 37, p. 1571 (1998).
[23] Wu J., Taylor K.E., Biswas N., Bewtra J.K., Kinetic Model for Removal of Phenol by Horseradish Peroxidase with PEG, J. Environ. Eng., 125, p. 451 (1999).
[24] B´odalo A., G´omez L., G´omez E., Bastida J., Hidalgo M., G´omez M., Yelo M., Elimination of 4-Chlorophenol by Soybean Peroxidase and Hydrogen Peroxide: Kinetic Model and Intrinsic Parameters, Bioch. Eng. J., 34, p. 242 (2007).
[25] G´omez L., G´omez E., Bastida J., Hidalgo M., G´omez M., Murcia D., Experimental Behaviour and Design Model of a Continuous Tank Reactor for Removing 4-Chlorophenol with Soybean Peroxidase, Chem. Eng. Proc., 47, p. 1786 (2008).
[26] Nazari K., Mahmoudi A., Khosraneh M., Haghighian Z., Moosavi-Movahedi A.A., Kinetic Analysis for Suicide-Substrate Inactivation of Microperoxidase-11: A Modified Model for Bisubstrate Enzymes in the Presence of Reversible Inhibitors, J. Molecular Catalysis B: Enzymatic, 56, p. 61 (2009).
[27] Michniewicz A., Ledakowicz S.,Ullrich R., Hofrichter M.,Kinetics of the Enzymatic Decolorization of Textile Dyes by Laccase from Cerrena unicolor, Dyes and Pigments, 77, p. 295 (2008).
[28] Zilly A., da Silva Coelho-Moreira J., Bracht A., Marques de Souza C.G., Elise Carvajal A., Angélica Koehnlein E., Marina Peralta R., Influence of NaCl and Na2SO4 on the Kinetics and Dye Decolorization Ability of Crude Laccase from Ganoderma lucidum,Int. Biodeterior.Biodegrad., 65, p. 340 (2011).
[29] Yousefi V., Kariminia H.-R., StatisticalAnalysis for Enzymatic Decolorization of Acid Orange 7 by Coprinus Cinereus Peroxidase. Int. Biodeter. Biodegr., 64, p. 245 (2010).
[30] Mansouri Majoumerd M., Kariminia H.-R., Investigation on Decolorization of Reactive Black 5 by Enzymatic Method, J. of Colour Sci. Technol. (In Persian), 5, p. 11 (2011).
[31] G´omez L., B´odalo A., G´omez E., Bastida J., Hidalgo M., G´omez M., A Covered Particle Deactivation Model and an Expanded Dunford Mechanism for the Kinetic Analysis of the Immobilized SBP/Phenol/Hydrogen Peroxide System, Chem. Eng. J., 138, p. 460 (2008).
[32] Ikehata K., Buchanan I., Pickard M.A., Smith D.W., Purification, Characterization and Evaluation of Extracellular Peroxidase from Two Coprinus Species for Aqueous Phenol Treatment, Bioresource Technol., 96, p. 1758 (2005).
[33] Nakayama T., Amachir T., Fungal Peroxidase: its Structure, Function, and Application, J. Molecular Catalysis B: Enzymatic, 6, p. 185 (1999).
[34] Patel P.K., Mondal M.S., Modi S., Behere D.V., Kinetic Studies on the Oxidation of Phenols by the Horseradish Peroxidase Compound II, Biochim. Biophys. Acta., 1339, p.79 (1997).
[35] Nicell J.A., Kinetic of Horseradish Peroxidase-Catalyzed Polymerization and Precipitation of Aqueous 4-chlorophenol, J. Chem. Technol. Biotechnol., 60, p. 203 (1994).
[36] Purich D.L., “Enzyme Kinetics: Catalysis & Control, A Reference of Theory and Best-Practice Methods”,  Elsevier Inc. (2010).
[37] Forgacsa E., Cserha´tia T., Orosb G., Removal of Synthetic Dyes from Waste waters: a Review, Environment International, 30, p. 953 (2004).
[38] Song H.Y., Yao J.H., Liu J.Z., Zhou S.J., Xiong Y.H., Ji L.N., Effect of Phthalic Anhydride Modification on Horseradish Peroxidase Stability and Structure, Enzyme Microb. Technol., 36, p. 605 (2005).
[39] Wang C., Yediler A., Lienert D., Wang Z., Kettrup A., Ozonation of an Azo Dye C.I. Remazol Black 5 and Toxicological Assessment of its Oxidation Products, Chemosphere, 52, p. 1225 (2003).