Optimization of Bioreactor Cultivation Parameters by Taguchi Orthogonal Array Design for Enhanced Prodigiosin Production

Document Type : Research Article

Authors

Faculty of Science, Mansoura University, Mansoura, EGYPT

Abstract

One of the major steps toward the industrialization of the microbial product(s) is to optimize the cultivation conditions at the large-scale bioreactor and successfully control the microbial behavior within a large scale production environment. Statistical Design of Experiment was proven to optimize a vast number of microbial processes to achieve robustness and explore possible interactions among the variables. In this research, Taguchi Orthogonal Array was applied to optimize the cultivation condition of a newly isolated Prodigiosin-producing marine bacterial strain, Serratia AM8887, at the bioreactor level. The two-steps fermentation process was applied; as the productivity was scaled up from shake flask level to a benchtop bioreactor (5L) and subsequently to an in-situ sterilization bioreactor system (20L); yielding a 7g/L of the pigment; compared to 100mg/L before optimization confirming that; applying Taguchi experimental design is a reliable and good positive option for the optimization of biotechnological processes. The produced pigment was purified and the chemical structure was revealed using Spectrophotometric, Mass Spectrum (MS), Fourier Transform InfraRed (FT-IR), and proton Nuclear Magnetic Resonance (1H-NMR) spectroscopy analysis. The biological activity including antibacterial (against multidrug resistance), antioxidants (against 1,1-diphenyl-2-picrilhydrazyl) and cytotoxicity to cancer cell lines (against breast cancer MCF-7 and liver cancer HepG-2) of the pigment were explored showing very characteristic features that could be helpful in food, pharmaceuticals, and/or textile industries

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References

[1] Cang S., Sanada M., Johdo O., Ohta S., Nagamatsu Y., Yoshimoto A., High Production of Prodigiosin  by Serratia Marcescens Grown on Ethanol, Biotechnology Letters, 22(22): 1761-1765 (2000).
[2] Sumathi C., MohanaPriya D., Swarnalatha S., Dinesh M., Sekaran G., Production of Prodigiosin Using Tannery Fleshing and Evaluating Its Pharmacological Effects, The Scientific World Journal, 2014:1-8 (2014).
[3] Maheswarappa G., Kavitha D., Vijayarani K., Kumanan K., Prodigiosin as Anticancer Drug Produced from Bacteria of Termite Gut, Indian Journal of Basic and Applied Medical Research, 1: 257-266 (2013).
[4] Tomás R.P., Ruir C.D., Montaner B., Prodigiosin Induces Cell Death and Morphological Changes Indicative of Apoptosis in Gastric Cancer Cell Line HGT-1, Histology and Histopathology, 16(2): 415-421 (2001).
[5] Elrazak A.A., Ward A.C., Glassey J., Polyunsaturated Fatty Acid Production by Marine Bacteria, Bioprocess and Biosystems Engineering, 36(11): 1641-1652 (2013).
[6] Fei N.C., Mehat N.M., Kamaruddin S., Practical Applications of Taguchi Method for Optimization of Processing Parameters for Plastic Injection Moulding: A Retrospective Review, ISRN Industrial Engineering, 2013: 1-11 (2013).
[7] Osman Y., Elrazak A.A., Khater W., Bioprocess Optimization of Microbial Biopolymer Production, Journal of Biobased Materials and Bioenergy, 10(2):119-128 (2016).
[8] Venil C., Lakshmanaperumalsamy P., Taguchi Experimental Design for Medium Optimization for Enhanced Protease Production by Bacillus subtilis HB04, e-Journal of Science & Technology, 4(4): 1-10 (2009).
[9] Yong-jun X., Lian-zhong A., Determination of Prodigiosin in Fermentation Broth by HPLC, Modern Food Science and Technology, 29(11): 2772-2776 (2013).
[10] Venil C.K., Lakshmanaperumalsamy P., An Insightful Overview on Microbial Pigment, Prodigiosin, Electronic Journal of Biology, 5(3): 49-61 (2009).
[11] Naik C., Srisevita J., Shushma K., Farah N., Shilpa A., Muttanna C., Darshan N., Sannadurgappa D., Peanut Oil Cake: a Novel Substrate for Enhanced Cell Growth and Prodigiosin Production from Serratia marcescens CF-53, Journal of Research in Biology, 2(6): 549-557 (2012).
[12] Mohammed H., Naseer J., Aruna K., Study on Optimization of Prodigiosin Production by Serratia marcescens Msk1 Isolated from Air, International Journal of Advanced Biotechnology Research, 2: 671-680 (2012).
[13] Namazkar S., Garg R., Ahmad W.Z., Nordin N., Production and Characterization of Crude and Encapsulated Prodigiosin Pigment, International Journal of Chemical Sciences and Applications, 4(3): 116-129 (2013).
[14] Casullo De Araújo H.W., Fukushima K., Takaki G.M.C., Prodigiosin Production by Serratia Marcescens UCP 1549 Using Renewable-Resources As A Low-Cost Substrate. Molecules, 15(10): 6931-6940 (2010).
[15] Ibrahim D., Nazari T.F., Kassim J., Lim S.H., Prodigiosin-an Antibacterial Red Pigment Produced by Serratia Marcescens IBRL USM 84 Associated with a Marine Sponge Xestospongia Testudinaria. Journal of Applied Pharmaceutical Science, 4(10): 1-6 (2014).
[16] Vora J., Jain N., Modi H., Identification and Characterization of Pigment Producing Strain Kocuria KM243757 & JO1 KM216829 from Kharaghoda Soil, International Journal of Current Microbiology and Applied Sciences, 4(9): 850-859 (2015).
[17] Mosmann T., Rapid Colorimetric Assay for Cellular Growth and Survival: Application to Proliferation and Cytotoxicity Assays, Journal of Immunological Methods, 65 (1):55-63 (1983).
[18] Vijayan P., Raghu C., Ashok G., Dhanaraj S., Suresh B., Antiviral Activity of Medicinal Plants of Nilgiris. Indian Journal of Medical Research, 120 : 24-29 (2004).
[19] Wang D.S., Koo T.Y., Lin I.-P., Liu H.-R., Chou C.-Y., Note: Determination of Nattokinase Production Condition Using Taguchi Parameter Design, Food Science and Technology International, 12 (3): 215-220 (2006).
[20] Oskouie S.F.G., Tabandeh F., Yakhchali B., Eftekhar F., Enhancement of Alkaline Protease Production by Bacillus Clausii Using Taguchi Experimental Design, African Journal of Biotechnology, 6(22):2559-2564 (2007).
[21] Mandal A., Kar S., Dutta T., Pati B.R., Mondal K.C., Mohapatra P.K.D., Parametric Optimization of Submerged Fermentation Conditions for Xylanase Production by Bacillus Cereus BSA1 Through Taguchi Methodology, Acta Biologica Szegediensis, 59 (2): 189-195 (2015).
[22] Shukla S., Goyal A., Development of Efficient Fermentation Process at Bioreactor Level by Taguchi's Orthogonal Array Methodology for Enhanced Dextransucrase Production From Weissella Confusa Cab3, Advances in Microbiology, 2 (3): 277-283 (2012).
[23] Alihosseini F., Lango J., Ju K.S., Hammock B. D., Sun G., Mutation of Bacterium Vibrio Gazogenes for Selective Preparation of Colorants, Biotechnology Progress, 26 (2), 352-360 (2010).
[24] Song M.J., Bae J., Lee D.S., Kim C.H., Kim J.S., Kim S.W., Hong S.I., Purification and Characterization of Prodigiosin Produced by Integrated Bioreactor from Serratia Sp. KH-95, Journal of Bioscience and Bioengineering, 101(2): 157-161 (2006).
[25] Guryanov I., Karamova N., Yusupova D., Gnezdilov O., Koshkarova L., Bacterial Pigment Prodigiosin and Its Genotoxic Effect, Russian Journal of Bioorganic Chemistry, 39 (1): 106-111 (2013).
[26] Ahmad W.A., Production and Characterization of Crude and Encapsulated Prodigiosin Pigment, International Journal of Chemical Sciences and Applications, 4 (3):116-129 (2013).
[27] Aruldass C.A., Venil C.K., Zakaria Z.A., Ahmad W.A., Brown Sugar As A Low-Cost Medium for the Production of Prodigiosin by Locally Isolated Serratia Marcescens UTM1. International Biodeterioration & Biodegradation, 95:19-24 (2014).
[28] Hassani H.H., Cytotoxicty and Dna Fragmentation in Cancer Cell Lines, Hep–2 and Amn–3, Induced by a Novel Maroon Pigment From Mutant Strain of Rugamonas Rubra Rr62, Journal of Al-Nahrain University, 14(3): 110-114 (2011)
[29] Athavale M.A., Somaiya S.S., More G.A., Satish S., Srivastava S.A., Comparitive In-Vitro Cytotoxicity of Red Pigment Extract of Serratia Marcescens on Breast and Prostrate Cancer Cell Lines, International Journal of Current Pharmaceutical Research, 5(2): 140-143 (2013)
[30] Hsieh H.Y., Shieh J.J., Chen C.J., Pan M.Y., Yang S.Y., Lin S.C., Chang J.S., Lee A.Y.L., Chang C.C., Prodigiosin Down‐Regulates SKP2 to Induce P27kip1 Stabilization and Antiproliferation In Human Lung Adenocarcinoma Cells, British Journal of Pharmacology, 166 (7): 2095-2108 (2012).
Iranian Journal of Chemistry and Chemical Engineering
Volume 39, Issue 3 - Serial Number 101
May and June 2020
Pages 319-330

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