Encapsulation of Alpha-Tocopherol Using Polycaprolactone and Tween20: Formulation and a perspective for scaling up using micro-channel

Document Type : Research Article


1 Department of Agro-Industrial Food and Environmental Technology, Faculty of Applied Science, King Mongkut’s University of Technology North Bangkok, Thailand

2 Department of Biotechnology, Faculty of Agro-industry, Kasetsart University, Thailand

3 Department of Chemical Engineering, Faculty of Engineering, Kasetsart University, Thailand

4 Department of Chemical Engineering Faculty of Engineering Kasetsart University


Alpha-tocopherol or vitamin E is well known for its beneficial properties for human health. This bioactive compound can be recovered from various agricultural resources through extraction and separation processes. In order to prolong its shelf-life and to maintain its bioavailability, the purified substance can be further encapsulated using biocompatible reagent. In this work, the encapsulation factors including the concentration of polycaprolactone (PCL), concentration of tween20, and the ratio of organic phase to aqueous phase were experimentally investigated. The Box–Behnken experimental design was employed to determine the optimal encapsulation condition in a small batch system. The results revealed that, at the adjusted optimal condition, 98.43% encapsulation was achieved using the concentrations of PCL of 6 g/L, concentration of tween20 of 0.5 g/L, and the ratio of organic phase to aqueous phase of 1:2. Based on the optimal condition of the batch process, the continuous micro-channel encapsulator was employed for continuous encapsulation with different residence times. For the residence time of 1 s, this system provided the encapsulation efficiency of 92.48% with outstanding productivity of 73.99 mg/mLmin. This work can be further developed to increase the production capacity via parallel processing of micro-channels.


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