Optimized Bioconversion of Soybean Meal Waste to Valued Biosurfactant by Pseudomonas Aeruginosa (PTCC 1074)

Document Type : Research Article


1 Department of Chemical Engineering, Faculty of Engineering, Shahid Bahonar University of Kerman, Kerman, I.R. IRAN

2 Fisheries Department, Faculty of Marine Sciences, Chabahar Maritime University, Chabahar, I.R. IRAN


Recently, microbial surface-active molecules called biosurfactants, have gained significant attention due to their structural diversity, biodegradability, low toxicity, and several environmental and industrial applications. However, despite their advantages, they are not widely used because of high production costs, which can be overcome by bioconversion of agro-industrial wastes as low-cost substrates. The current study aimed to overcome the challenges of biosurfactant production by bioconversion of soybean meal, as a low-cost renewable substrate, and to optimize the significant parameters. Rhamnolipid biosurfactant was produced by Pseudomonas aeruginosa (PTCC 1074) using soybean meal under solid-state fermentation and Response Surface Methodology (RSM) by Central Composite Design (CCD) was employed to optimize the significant parameters. The experimental value of biosurfactant production and Emulsification Index were 17.05 (g/kg dry substrate) and 54 % respectively under the optimal conditions (temperature 33 ºC, Initial substrate moisture 80%, and carbon-to-nitrogen ratio (C/N ratio) 54). Regression analysis with RSM resulted in quadratic models and the coefficient of determination (R2), adjusted R2, and predicted R2 were respectively calculated as 0.9767, 0.9557, and 0.9088, indicating that the model fitted the experimental data well.  An increase in temperature from 25 to 34°C led to a rise in rhamnolipid production, which implies the significant influence of temperature. The results demonstrated that  the production of biosurfactants increased with increasing the initial moisture content at high temperatures and also at low C/N ratios. The current study confirmed the considerable potential of soybean meal for biosurfactant production and also enhanced the production yield by optimizing the significant process parameters.


Main Subjects

[1] Mulligan C.N., Environmental Applications for Biosurfactants, Environ. Pollut, 133:183–198 (2005).
[2] Gudina E.J., Teixeira J.A., Rodrigues L.R., Isolation and Functional Characterization of a Biosurfactant Produced by Lactobacillus Paracasei, Colloids Surf. B, 76: 298–304 (2011).
[3] Mahmoodabadi M., Khoshdast H., Shojaei V., Efficient Dye Removal from Aqueous Solutions Using Rhamnolipid Biosurfactants by Foam Flotation, Iran. J. Chem. Chem. Eng. (IJCCE), 38(4): 127-140 (2019).
[4] Desai J.D., Banat I.M., Microbial Production of Surfactants and their Commercial Potential, Microbiol Mol Biol R, 61:47–64 (1997).
[5] Hari R.M., Sahu A.K., Said M.S., Banpurkar A.G., Gajbhiye J.M., Dastager S.G., A Novel Fatty Alkene from Marine Bacteria: A Thermo Stable Biosurfactant and its Applications, J. Hazard. Mater. 380: 120868 (2019).
[6] Marchant R., Banat I.M., Microbial Biosurfactants: Challenges and Opportunities for Future Exploitation, Trends Biotechnol, 11: 558–565 (2012).
[7] Salehizadeh H., Mohammadizad S., Microbial Enhanced Oil Recovery Using Biosurfactant Produced by Alcaligenes faecalis, Iran J Biotech, 7(4): 216-223 (2009).
[8] Souza E.C., Christina T., Vessoni-Penna T.C., de Souza Oliveira R.P., Biosurfactant-Enhanced Hydrocarbon Bioremediation: An Overview, Int. Biodeterior. Biodegradation, 89: 88-94 (2014).
[9] Rodrigues L., Banat I.M., Teixeira J., Oliveira R., Biosurfactants: Potential Applications in Medicine,  J. Antimicrob. Chemother, 57: 609–618 (2006).
[10] Adu S.A., Naughton P.J., Marchant R., Banat I.M., Microbial Biosurfactants in Cosmetic and Personal Skincare Pharmaceutical Formulations, Pharmaceutics, 12: 1099 (2020).
[11] Nitschke M., Costa S.G.V.A.O., Biosurfactants in Food Industry, Trends Food Sci. Tech., 18: 252-259 (2007).
[12] Makkar R.S., Cameotra S.S., Banat I.M., Advances in Utilization of Renewable Substrates for Biosurfactant Production, Appl Microbiol Biotechnol, 11(1): 1–5 (2011).
[13] Dziegielewska E., Adamczak M., Evaluation of Waste Products in the Synthesis of Surfactants by Yeasts, Chem. Pap, 67: 1113–1122 (2013).
[14] Pandey A., Solid-State Fermentation, Biochem, Eng. J, 13: 81–84 (2003).
[17] Jajor P., Piłakowska-Pietras D., Krasowska A., Łukaszewicz M., Surfactin Analogues Produced by Bacillus subtilis strains Grown on Rapeseed Cake, J. Mol. Struct., 1126:141–146 (2016).
[18] Lourenço L.A, Alberton-Magina M.D., Tavares L.B.B., Guelli-Ulson de Souza S.M.A., García-Román M., Altmajer-Vaz D., Biosurfactant Production by Trametes Versicolor Grown on Two-Phase Olive Mill Waste in Solid-State-Fermentation, Environ. Technol,. 39: 3066–3076 (2018).
[19] Jimenez-Peñalver P., Castillejos M., Koh A., Gross R., Font X., Sánchez A., Production and Characterization of Sophorolipids from Stearic Acid by Solid-State Fermentation, A Cleaner Alternative to Chemical Surfactants. J. Clean. Prod., 172: 2735–2747 (2018).
[20] El‑Housseiny G.S., Aboshanab K.M., Aboulwafa M.M., Hassouna N.A., Rhamnolipid Production by A Gamma Ray‑Induced Pseudomonas aeruginosa Mutant under Solid State Fermentation, AMB Exp., 9: 7 (2019).
[21] Kreling N.E., Simon V., Fagundes V.D., Thomé A., Colla L.M., Simultaneous Production of Lipases and Biosurfactants in Solid-State Fermentation and Use in Bioremediation. J. Environ. Eng., 146(9): 04020105 (2020).
[23] Mouafi F.E., Elsouda M.M.A., Moharamb M.E., Optimization of Biosurfactant Production by Bacillus brevis Using Response Surface Methodology, Biotechnol. Rep., 9: 31–37 (2016).
[24] Pereira J.F.B., Gudina E., Costa R., Vitorino R., Teixeira J., Coutinho J., Optimization and Characterization of Biosurfactant Production by Bacillus subtilis isolates Towards Microbial Enhanced Oil Recovery Applications, Fuel, 111: 259–268 (2013).
[25] Neto D.C., Meira J.A., Araújo J.M., Mitchell D.A., Krieger N., Optimization of the Production of Rhamnolipids by Pseudomonas aeruginosa UFPEDA 614 in Solid-State Culture, Appl. Microbio.l Biotechnol., 81: 441–448 (2008).
[26] Neto D.C., Bugay C., Santana-Filho A.P., Joslin T., Souza L.M., Sassaki G.L., Mitchell D.A., Krieger N., Production of Rhamnolipids in Solid-State Cultivation Using a Mixture of Sugarcane Bagasse and Corn Bran Supplemented with Glycerol and Soybean Oil, Appl. Microbiol. Biotechnol, 89: 1395–1403 (2011).
[28] Tahzibi A., Kamal F., Mazaheri Assadi M., Improved Production of Rhamnolipids by a Pseudomonas aeruginosa Mutant, Iranian Biomedical Journal, 8(1): 25-31 (2004).
[29] Cooper D.G., Goldenberg B.G., Surface-Active Agents from Two Bacilllus Species, Appl. Environ. Microbiol, 53: 224 (1987).
[32] Draper N., John J.A., Response Surface Design for Quantitative and Qualitative Variables, Technometrics, 30:423–428 (1988).
[33] Babu K.R., Satyanarayana T., Amylase Production by Thermophilic Bacillus Coagulance in Solid-State Fermentation, Proc. Biochem., 30: 305-309 (1995).
[34] Manivasagan P., Sivasankar P., Venkatesan J., Sivakumar K., Kim S.K., Optimization, Production and Characterization of Glycolipid Biosurfactant from the Marine Actinobacterium, Streptomyces sp. MAB36, Bioprocess Biosyst Eng., 37: 783–797 (2014).
[35] Najafi A.R., Rahimpour M.R., Jahanmiri A.H., Roostaazad R., Arabian D., Soleimani M., Jamshidnejad Z., Interactive Optimization of Biosurfactant Production by Paenibacillus alvei ARN63 Isolated from an Iranian Oil Well, Colloids Surf B, 82: 33–39 (2011).
[36] Zadrazil F., Brunnert H., Investigation of Physical Parameters Important for the SSF of Straw by White Rot Fungi, Eur. J. Appl. Microbiol. Biotechnol., 11:183-188 (1981).
[37] Rashedi H., Mazaheri Asadi M., Jamshidi E., Bonakdarpour B., Optimization of the Production of Biosurfactant by Psuedomonas Aeruginosa HR Isolated from an Iranian Southern Oil Well, Iran. J. Chem. Chem. Eng. (IJCCE), 25(1): 25-30 (2006).
[38] Krishna C., Nokes S.E., Influence of Inoculum Size on Phytase Production and Growth in Solid-State Fermentation by Aspergillus niger, Transactions of the ASAE, 44(4): 1031-1036 (2001).