Evaluation of the Physicochemical Properties and Aflatoxin Levels of Industrial and Non-industrial Sesame Oil

Document Type : Research Article


1 Department of Nutrition, School of Medicine, Mashhad University of Medical Sciences, Mashhad, I.R. IRAN

2 Department of Food Science and Technology, Faculty of Agriculture, Ferdowsi University of Mashhad, Mashhad, I.R. IRAN

3 Department of Nutrition, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, I.R. IRAN


The present study aimed to evaluate the physicochemical properties (refractive index, acid value, peroxide value, conjugated dienes, Fatty acid composition) and level of aflatoxins (AFB1, AFB2, AFG1, and AFG2) in the sesame oils (cold press) consumed in Iran. In total, 30 sesame oil samples were collected from factories (n=20; industrial) and traditional mills (n=10; non-industrial). No significant differences were observed between the industrial and non-industrial sesame oil samples in terms of the physicochemical properties and AF contamination and therefore, it is not possible to prefer the consumption of one of the oils (industrial or non-industrial) to another. According to the physicochemical examination, the mean peroxide value was 2.93±1.59 and 1.95±1.24 meq/kg, the acid value was 0.86±0.82 and 1.12±0.58 mg KOH/g, the refractive index was 1.4,706±0.0002 and 1.4,705±0.0001 at 28°C, and the conjugated diene value was 12.13±3.25 and 10.02±1.43 μmol/g in the industrial and non-industrial sesame oil, respectively. In addition, the fatty acid profile of the industrial and non-industrial sesame oil indicated high levels of unsaturated fatty acids (84.5% and 83.49%, respectively), with the main fatty acids determined to be oleic acid and linoleic acid. The fatty acid profile of the sesame oil samples indicated no adulteration with other vegetable oils. The mean contamination with AFB1, AFB2, and AFG1 in the non-industrial sesame oil was estimated at 0.06±0.26, 0.02±0.67, and 0.15±0.18 µg/kg, while the mean contamination with AFB1, AFB2, and AFG1 was 0.04±0.84, 0.03±0.61, and 0.17±0.16 µg/kg in the industrial sesame oil. Moreover, the AFB1 and AFs levels in all the sesame oil samples were significantly lower than the Iranian legislation limits (5 and 15 µg/kg, respectively) and the European Union (2 and 4 µg/kg, respectively). Risk assessment based on the margin of exposure revealed the risk of AFB1 and AFG1 exposure through industrial and non-industrial sesame oil consumption and AFB2 exposure through industrial sesame oil consumption.


Main Subjects

[2] Ram R., Catlin D., Romero J., Cowley C., Sesame: New Approaches for Crop Improvement, "Advances in New Crops Timber Press", Portland, 225-228 (1990).
[3] Borchani C., Besbes S., Blecker C., Attia H., Chemical Characteristics and Oxidative Stability of Sesame Seed, Sesame Paste, and Olive Oils, J. Agric. Sci. Technol., 12: 585-596 (2010).
[5] Morris J.B., Food, Industrial, Nutraceutical, and Pharmaceutical Uses of Sesame Genetic Resources. "Trends in New Crops and New Uses", 153-156 (2002).
[6] Cooney R.V., Custer L.J., Okinaka L., Franke A.A., Effects of Dietary Sesame Seeds on Plasma Tocopherol Levels, Nutr Cancer., 39(1): 66-71 (2001).
[7] Abou-Gharbia H.A., Shehata A.A.Y., Shahidi F., Effect of Processing on Oxidative Stability and Lipid Classes of Sesame Oil, Food Res. Int., 33(5): 331-340 (2000).
[8] Mohammed F., Abdulwali N., Guillaume D., Tenyang N., Ponka R., Al-Gadabi K., Bchitou R., Abdullah A.H., Mohammed Naji Kh., Chemical Composition and Mineralogical Residence of Sesame Oil from Plants Grown in Different Yemeni Environments, Microchem. J., 140: 269-277 (2018).
[9] El Khier M.K.S., Ishag K.E.A., Yagoub A., Chemical Composition and Oil Characteristics of Sesame Seed Cultivars Grown in Sudan, Res. J. Agric. & Biol. Sci., 4(6): 761-766 (2008).
[10] Gharby S., Harhar H., Bouzoubaa Z., Asdadi A., El Yadini A., Charrouf Z., Chemical Characterization and Oxidative Stability of Seeds And Oil of Sesame Grown in Morocco, J. Saudi Soc. Agric., 16(2): 105-111 (2017).
[11] Hilali M., Charrouf Z., Aziz Soulhi A.E., Hachimi L., Guillaume D., Influence of Origin and Extraction Method on Argan Oil Physico-Chemical Characteristics and Composition, J. Agric. Food Chem. J., 53(6): 2081-2087 (2005).
[12] Diwakar B.T., Dutta P.K., Lokesh B.R., Naidu KA., Physicochemical Properties of Garden Cress (Lepidium sativum L.) Seed Oil, Journal of the American Oil Chemists' Society, 87(5): 539-548 (2010).
[13] Rahman M., Hossain M., Ahmed G., Uddin M., Studies on the Characterization, Lipids and Glyceride Compositions of Sesame (Sesamum indicum linn.) Seed Oil, Bangladesh J. Sci. Ind. Res., 42(1): 67-74 (2007).
[14] Moricz A.M., Fater Z., Otta K.H., Tyihak E., Mincsovics E., Overpressured Layer Chromatographic Determination Of Aflatoxin B1, B2, G1 and G2 in Red Paprika, Microchemical Journal., 85(1): 140-144 (2007).
[15] Mohammed S., Munissi J.J.E., Nyandoro S.S., Aflatoxins in Sunflower Seeds and Unrefined Sunflower Oils From Singida, Tanzania, Food Addit Contam: Part B., 11(3): 161-166 (2018).
[16] Rai MK, Bonde SR, Ingle AP, Gade AK., Mycotoxin: Rapid Detection, Differentiation and Safety, J. Adv. Pharm. Educ. Res., 3(1): (2012).
[17] Magzoub R., Yassin A., Abdel-Rahim A., Gubartallah E., Miskam M., Saad B., Sabar S., Photocatalytic Detoxification of Aflatoxins in Sudanese Peanut Oil Using Immobilized Titanium Dioxide, Food Control., 95: 206-214 (2019).
[18] Sweeney M.J., Dobson A.D., Mycotoxin Production by Aspergillus, Fusarium and Penicillium Species, Int. J. Food Microbiol., 43(3):141-158 (1998).
[21] Elbashir A.A., Ali S.E.A., Mycotoxin Risk Assessment for the Purpose of Setting International Regulatory Standards, Food Addit Contam: Part B., 7(2): 135-140 (2014).
[22] Society A.O.C., Firestone D., Official Methods and Recommended Practices of the American Oil Chemists' Society: AOCS press; (1994).
[23] International IDF Standards International Dairy Federation., IDF-Square Vergote 41. Brussels SA (1991).
[24] Saguy I., Shani A., Weinberg P., Garti N., Utilization of Jojoba Oil for Deep-Fat Frying of Foods, LWT-Food Science and Technology., 29(5-6):573-577 (1996).
[25] Society AOC. Official Methods and recommended Practices of the American Oil  Chemists of Analysis: 7 th ed., AOCS-Pr.Champaign IL; (2020).
[26] Christie WW., Preparation of Ester Derivatives of Fatty Acids for Chromatographic Analysis, Advances in Lipid Methodology., 2(69): e111 (1993).
[27] Zio S., Dembele I., Bazié R.S.B., Tankoano A., Tapsoba F., Hama-Ba F., et al. Aflatoxins and Moisture Levels in Edible Oils Produced in Burkina Faso, E European J. Nutr. Food Saf., 1-12 (2020).
[28] Gotoh N., Wada S., The Importance of Peroxide Value in Assessing Food Quality and Food Safety, JAOCS, Journal of the American Oil Chemists' Society, 83(5): 473 (2006).
[29] Birjandi M., Sepahvand R., Hassanzadazar H., Hatamikia M., Kazemi-Vardanjani A., A Survey on Peroxide Content and Acidity Level of Persian Doughnuts in Lorestan Province, West of Iran, J. Glob. Pharma Technol., 10(8): 32-35 (2016).
[30] Gadade B., Kachare D., Satbhai R., Naik R., Nutritional Composition and Oil Quality Parameters Of Sesame (Sesamum indicum L.) Genotype, Int. Res. J. Multidiscip Stud., 3(7): 2454-8499 (2017).
[32] Institute of Standards and Industrial Research of Iran. E”dible Cold Pressed Oils - Specifications & Test Methods. 13392": 1st. Revision,; (2015).
[33] Alimentarius C., Codex Standard for Named Vegetable Oils, Codex Stan 210 2005.
[34] Mohamed Ahmed I.A., Musa Özcan M., Uslu N., Juhaimi F.A., Osman M.A., Alqah H.A., et al.  Effect of Microwave Roasting on Color, Total Phenol, Antioxidant Activity, Fatty Acid Composition, Tocopherol, And Chemical Composition of Sesame Seed and Oils Obtained From Different Countries, J. Food Process. Preserv., 44(10): e14807 (2020).
[35] Ahmed I.A.M., Uslu N., Özcan M.M., Juhaimi F.A., Ghafoor K., Babiker E.E., et al. Effect of Conventional oven Roasting Treatment on the Physicochemical Quality Attributes of Sesame Seeds Obtained From Different Locations, Food Chemistry, 338: 128109 (2021).
[38] Institute of Standards and Industrial Research of Iran. “Crude Sesame Oil -Specifications and Test Methods, No 8636". 1st ed, 1st Revision, (2014).
[40] Pandurangan M., Murugesan S., Gajivaradhan P., Physico-Chemical Properties of Groundnut Oil and their Blends with Other Vegetable Oils, J. Chem. Pharm. Res., 6(8): 60-66 (2014).
[41] Olaleye A.A., Adamu Y.A., Lawan U., Effects of Temperature Change on the Physico-Chemical Properties of Sesame Seed Oil, Sci. J. anal. Chem., 7(1): 13 (2019).
[42] Godswill A.C., Amagwula I.O., Victory I.S., Gonzaga A.I., "Effects of Repeated Deep Frying on Refractive Index and Peroxide Value of Selected Vegetable Oils". (2018).
[43] Al-Bachir M., Othman Y., Comparative Studies on Some Physicochemical Properties of Oil Extracted from Gamma Irradiated Sesame (Sesamum Indicum L.) Seeds, Journal of Food Chem and Nanotechnology, 5(2): 36-42 (2019).
[44] Aparicio R., Roda L., Albi M.A., Gutiérrez F., Effect of Various Compounds on Virgin Olive Oil Stability Measured by Rancimat, J. Agric. Food Chem. J., 47(10): 4150-4155 (1999).
[45] Sadeghi E., Mahtabani A., Karami F., "Considering the Oxidative Stability of Cold-Pressed Sesame, Sunflower, and Olive Oils under Different Storage Conditions", The Annals of the University Dunarea de Jos of Galati Fascicle VI-Food Technology, 43(2):70-83 (2019).
[46] Bialek A., Bialek M., Jelinska M., Tokarz A., Fatty Acid Composition and Oxidative Characteristics of Novel Edible Oils in Poland, CyTA-Journal of Food, 15(1): 1-8 (2017).
[47] Foster R.H., Hardy G., Alany R.G., Borage Oil in the Treatment of Atopic Dermatitis, Nutrition. 26(7-8): 708-718 (2010).
[48] Ahmed I.A.M., AlJuhaimi F., Özcan M.M., Ghafoor K., Şimşek Ş., Babiker E.E., et al. Evaluation of Chemical Properties, Amino Acid Contents and Fatty Acid Compositions of Sesame Seed Provided from Different Locations, J. Oleo Sci. 2020:ess20041.
[50] Borchani C., Besbes S., Blecker C., Attia H., Chemical Characteristics and Oxidative Stability of Sesame Seed, Sesame Paste, and Olive Oils, J. Agric. Sci. Technol., (2010).
[51] Elzupir A.O., Suliman M.A., Ibrahim I.A., Fadul M.H., Elhussein AM., Aflatoxins Levels in Vegetable Oils in Khartoum State, Sudan, Mycotoxin Res., 26(2): 69-73 (2010).
[52] Iranian National Standardization Organization. "Maximum Tolerated limits of Mycotoxins in Foods and Feeds, No. 5925", Tehran, Iran: Institute of Standard and Industrial Research of I.R. Iran; (2002). (Persian).
[54] Environmental Protection Agency (EPA), “Exposure Factors Handbook”: 2011 Edition. EPA/600/R-09/052F, US EPA, Office of Research and Development Washington, DC; (2011).
[55] Institute of Standards and Industrial Research of Iran. Food & Feed-Maximum Limit of Heavy Metals 12968, 1st ed.
[56] Ghasemidehkordi B., Malekirad A.A., Nazem H., Fazilati M., Salavati H., Shariatifar N., et al. Concentration of Lead and Mercury in Collected Vegetables And Herbs from Markazi province, Iran: a Non-Carcinogenic Risk Assessment, Food Chem. Toxicol., 113: 204-210 (2018).
[57] Environmental Protection Agency (EPA). Risk Assessment Guidance for Superfund. EPA/540/1-89/002; 2004.
[59] Joint F.A.O., WHO Expert Committee on Food Additives, "Evaluation of Certain Food Additives and Contaminants: Sixty-Eighth Report of the Joint FAO/WHO Expert Committee on Food Additive", Technical. Report. Series., No 947. WHO, Geneva, Switzerland, pp. 169e180; (2007).