Average Shear Rate Estimation in Conventional Stirred-Tank Bioreactor Using Non-Newtonian Fluid: An Experimental Approach

Rewaria, Vinay; Mathi, Pardhasaradhi; Botlagunta, Mahendran

doi:10.30492/ijcce.2022.543342.5025

Average Shear Rate Estimation in Conventional Stirred-Tank Bioreactor Using Non-Newtonian Fluid: An Experimental Approach

Document Type : Research Article

Authors

¹ Biomedical Research Laboratory, Department of Biotechnology, K L E F University (Koneru Lakshmaiah Educational Foundation), Greenfields; Vaddeswaram, Guntur (Dist), Andhra Pradesh 522502, INDIA

² Upstream Process Development Lab, Gene therapy department, Reliance life sciences, Navi Mumbai, Maharashtra, 400701 INDIA

10.30492/ijcce.2022.543342.5025

Abstract

An experimental approach for the estimation of average shear rate (γ_av) in a stirred tank bioreactor has been proposed for the turbulent flow regime. Based on the proposed methodology, the correlation for the estimation of γ_avwas obtained as a function of agitation speed (N), superficial gas velocity (Vs), and the rheological properties of the non-Newtonian fluids. The γ_avestimated by the present method was found to be within the range of values calculated by correlations available in the literature. The γ_avincreased with the increase of agitation and sparging in all the conditions tested. The correlation derived in the present work helps in the estimation of γ_av as a function of bioreactor geometry along with physical conditions (N and Vs), and rheological properties (n and K) of non-Newtonian fluid in commercially available stirred tank bioreactor.

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