Synthesis of Green Zinc Oxide Nanoparticles Mediated by Syzygium cumini Induced Developmental Deformation in Embryo Toxicity of (Daniorerio) Zebrafish

Document Type : Research Article


1 Sri Paramakalyani College, Manonmaniam Sundaranar University, Alwarkurichi - 627412, INDIA

2 Sri Paramakalyani Centre of Excellence in Environmental Sciences, Manonmaniam Sundaranar University, Alwarkurichi - 627412, INDIA

3 Saveetha Dental College and Hospitals, SIMATS, Chennai – 600077, TN, INDIA


This study investigated the synthesis of Zinc oxide (ZnO) nanoparticles using Syzygium cumini fruit (Indian blackberry) seed extract. The seeds extract of Syzygium cumini fruit have properties of anti-diabetic, anti-inflammatory, and anti-bacterial, and traditionally it has long been used in Indian folklore medicine. Zebrafish embryos and larvae were treated with 5 different concentrations (0, 25, 50, 75, 100, 150 µg/mL ) of Zinc oxide (ZnO) nanoparticles from 4 hours post fertilization (hpf). The results showed that exposure to 50-150 µg/mL zinc oxide nanoparticles (ZnO NPs) induced developmental toxicity in these embryos, causing mortality, hatching delay, and malformation. Exposure of 50-150 µg/mL  Zinc oxide (ZnO) nanoparticles to zebrafish embryo caused coagulated unhatched phenotype, spinal curvature, axis bent, tail malformation, yolk sac, and pericardial edema, at 72-96 hpf. These results will assist in elucidating the mechanisms of the developmental toxicity of green synthesized Zinc oxide nanoparticles during the embryonic development of zebrafish.


Main Subjects

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