Evaluation of Microbial and Sensory Properties of Jug Cheese Packed in the Biodegradable Film of Polyvinyl Alcohol and Pinto Bean Starch-Containing Garlic, Ginger, and Cinnamon Essential Oils

Document Type : Research Article


Department of Food Science and Technology, College of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Varamin, I.R. IRAN


IBiodegradability and antimicrobial activity of food packaging materials are the most important parameters of modern food packaging industries. Therefore, the present study aimed to use a biodegradable film of polyvinyl alcohol and pinto bean starch-containing cinnamon, garlic, and ginger essential oils to increase the shelf life and reduce the microbial load of the jug cheese and compare with conventional packaging. For this purpose, jug cheese in the biodegradability film of polyvinyl alcohol/pinto bean starch (20/80%) respectively and dissolved in distilled water for 60 minutes at 90 °C was heated at 400 rpm stir. T10% glycerol was added to the filtered solutions on a magnetic stirrer (400 rpm) for 30 minutes at 37 °C to obtain a completely homogeneous solution. To make an antimicrobial biocomposite, three concentrations of each of the essential oils containing different concentrations (3.125, 6.25, and 12.5%) of cinnamon, garlic, and ginger The prepared films were poured into Teflon containers with a diameter of 15 cm and exposed to ambient temperature for 24 hours. The dried film layers were removed from the Teflon surface and stored in a zip kept at
25 °C until their microbial and sensory properties were evaluated during 60 days of storage at 4 ° C and compared with the control sample.  The results showed that the use of biodegradable film containing essential oil significantly reduced the microbial load in jug cheese samples. Total count of microorganisms, amount of Staphylococcus aureus, coliform, mold, and yeast in samples of jug cheese packed in a biodegradable film containing 6.25 and 12.5% cinnamon essential oil and 12.5% ginger essential oil after 60 days of storage was within the acceptable national standard. The highest general acceptance score among the acceptable samples of national standard belonged to the sample of jug cheese packed in a biodegradable film containing 6.25% of cinnamon essential oil. The use of biodegradable films based on pinto bean starch and polyvinyl alcohol along with cinnamon and ginger essential oils are solutions that can reduce the microbial load and increase the safety of food products during storage and prevent environmental damage.


Main Subjects

[1] Nateghi L., Movassaghi Nejad M., “Investigation of Antimicrobial Effects of Essential Oils and Natural Extracts in Food”, 10th National Conference on Agriculture and Sustainable Natural Resources, Tehran, (2019).
[2] Misaghi A., Saeedi M., Nouri N., Rezaei Golestani M.R., Study of the Effect of Oregano Essential Oil And Ethanolic Extract of Propolis on Antibacterial Properties and Some Physical Properties of Biodegradable Polylactic Acid Films, J Health Enviro (Scientific Research Quarterly of Iranian Scientific Association of Environmental Health). 11(1): 111-122 (2018).
[4] Razavi Rohani S.M., Moradi M., Mehdizadeh T., Saei-Dehkordi S.S., Griffiths M.W., The Effect of Nisin and Garlic (Allium Sativum L.) Essential Oil Separately and in Combination on the Growth of Listeria Monocytogenes, LWT-Food Sci Technol., 44: 2260-2265 (2011).
[5] Kumar B., Vijayakumar M., Govindarajan R.  Pushpangadan P., Ethnopharmacological Approaches to Wound Healing Exploring Medicinal Plants of India, J Ethnopharmacol., 114: 103-130 (2007).
[6] Dashti-Rahmatabadi M.H., Vahidi Merjugdi A.R., Pilavaran A.A., Farzan F., Antinociceptive Effect of Cinnamon Extract on Formalin-Induced Pain in the Rat, Shahid Sadoughi Uni. Medi. Sci., 17: 190-99 (2009).
[7] Mousavian S.M., Bazgir E., Moradpour A., Ingredients in Cinnamon Peel Essential Oil and its Antifungal Properties Against Fruit Rot Fungi, Best Agri., 19(4): 907-920 (2017).
[8] Gurib-Fakim A., Medicinal Plants: Traditions of Yesterday and Drugs of Tomorrow, Molecul Aspects Medic., 27: 1-93 (2006).
[9] Ojagh S.M., Rezaei M., Razavi S.H., Hosseini, S.M.H., Study of Antibacterial Effect of Cinnamon Peel Essential Oil (Cinnamomum zeylanicum) in Vitro Against Five Bacteria Causing Food Spoilage, Quarterly J Food Sci Technol. 35(9): 67-76 (2012).
[10] Han C., Wang J., Li Y., Lu F., Cui Y., Antimicrobial-Coated Polypropylene Films with Polyvinyl Alcohol in the Packaging of Fresh Beef, Meat Sci., 96: 901–907 (2014).
[11] Mesomo M.C., Corazza M.L., Ndiaye P.M., Dalla Santa O.R., Cardozo L., Scheer A.D.P., Supercritical CO2 Extracts And Essential Oil of Ginger (Zingiber o_cinale R.): Chemical Composition and Antibacterial Activity, J. Supercritical Fluids., 80: 44–49 (2013).
[12] Hasas B., Nateghi L., Lavasani Shahab A.R., Investigation of Physicochemical, Microbial, and Sensory Properties of Low-Fat Jug Cheese Containing Beta-Glucan Powder and Ethanolic Extract of Oregano, Iranian J. Food Sci. Technol. Res.,15(1): 181-198 (2019).
[13] Abdolsatari P., Pighambar Doost S.H., Molaie A., Peighambar Doost S.J., Hashemi M., Active Packaging of Lighvan Cheese with Polyethylene Nanocomposite Films Containing Metal Nanoparticles and Modeling the Migration of Silver Nanoparticles, Iranian Biosys Eng., 47(4): 667- 676 (2016).
[14] Nadaroglu H., Alayli A., Ceker S., Ogutcu H., Agar G., Biosynthesis of Silver Nanoparticles and Investigation of Genotoxic Effects and Antimicrobial Activity, Int. J. Nano Dimension, 11(2): 158–167 (2020).
[15] Nadaroglu H., Alayli A., Ceker S., Ogutcu H., Agar G., Investigation of Antimicrobial and Genotoxic Effects of Fe2O3, NiO and CoO NPs Synthesized by Green Synthesis, J. Nanoanalysis, [In Press]. (2020).
[17] Jayakumar A., Heera K.V., Sumi T.S., Joseph M., Starch-PVA Composite Films with Zinc-Oxide Nanoparticles and Phytochemicals as Intelligent pH Sensing Wrap for Food Packaging Application, International J. Biologi Macromolecular. (2019).
[18] Khazai A., Nateghi L., Zand, N., Abdulrasoul Oromiehie A., Garavand F.,  Evaluation of Physical, Mechanical and Antibacterial Properties of Pinto Bean Starch-Polyvinyl Alcohol Biodegradable Films Reinforced with Cinnamon Essential Oil, Polymer., 13(16): 2778 (2021).
[19] Iranian Institute of Standards and Industrial Research, “Food chain Microbiology, a Comprehensive Method for Counting Microorganisms, Part 1- Colony Count at 30 °C Using Mixed Culture Method”, Iranian National Standard, No 5484 (2014).
[21] Iranian Institute of Standards and Industrial Research, “Microbiology of Food and Animal Feed, a Comprehensive Method for Identifying and Counting Coliforms”, Iranian National Standard. No 11166 (2008).
[22] Iranian Institute of Standards and Industrial Research, “Milk and Its Products, Escherichia Coli Count, Most Probable Number Method (MPN)”, Iranian National Standard. No 5234 (2015).
[23] Iranian Institute of Standards and Industrial Research, “Milk and Its Products, Counting the Constituent Units of Mold and Yeast Colonies - Counting Colonies on a Plate at a Temperature of 25 ° C”, Iranian National Standard. No 10154 (2007).
[24] Hosseini M., Habibi Najafi, M., B. Mohebbi M., Evaluation of Physical, Chemical, and Sensory Properties of Imitation Cheese Containing Whey Protein Concentrate and Enzymatically Modified Lighvan Cheese, Iran. J. Nutr Sci Food Indus., 2(8): 91- 102 (2013).
[25] Iranian Institute of Standards and Industrial Research, Microbiology of Milk and Its Products - Characteristics and Test Methods, Iranian National Standard. No 2406. Amendment 1 (2019).
[26] Shan B., Cai Y., Brooks J. D., Corke H., The In Vitro Antibacterial Activity of Dietary Spice and Medicinal Herb Extracts, Int. J. Food Microb., 117: 112-119 (2007).
[27] Little C.L., RhoadesJ.R., Sagoo S.K., Harris j., Greenwood M., Mithani V., Identification and Biotyping of Coagulase-Positive Staphylococci Unripened Franch Raw Milk Cheese and there in Virto Ability to Production Enterotoxin, Revue Med Vet Journal Impacts Factor, 155: 92-96 (2006).
[28] Mahmoudi R., Ehsani A., Tajik H., Akhondzadeh Basti A., Khosroshahi Asl A., Antimicrobial Effect of Oregano Essential Oil and Lactobacillus Casey on Staphylococcus Aureus in Iranian White Cheese, J. Food Indus. Res., 3(1): 147-160 (2010).
[29] Akhundzadeh Basti A., Abbasifar A., Giti K., Misaghi A., Bukai S., Gandami Nasrabadi H., Jebli Javan A., Hamedi H., Salari A.A., Evaluation of the Effect of Shirazi Multiflora Essential Oil on the Behavior of Staphylococcus aureus in Feta Cheese, J. Medicin. Plants, 7(25): 105-115 (2007).
[30] Chang S.T., Chen P.F., Chang S.C., Antibacterial Activity of Leaf Essential Oils and Their Constituents from Cinnamomum Osmophloeum, J. Ethnopharmaco., 77(1): 123-127 (2001).
[31] Bozin B., Mimica-Dukic N., Samojlik I., Jovin, E., Antimicrobial and Antioxidant Properties of Rosemary and Sage (Rosmarinus officinalis L. and Salvia officinalis L., Lamiaceae) Essential Oils, J. Agri. and Food Chem., 55(19): 7879–7885 (2007).
[32] Vaziri S., Norouzi M., The Study of the Contamination of Local Lighvan Tabriz Cheeses with Coliforms and Escherichia coli in Maragheh, Iranian Journal of Medical Microbiology, 7(5): 23-28 (2011).
[33] Hayaloglu A.A., Fox P.F., Cheese of Turkey, Varieties Containing Herbs or Spices, Dairy Sci Technol., 88: 245- 256 (2008).
[35] Krisch J., Tserennadmid R., Vágvölgy C., Essential Oils Against Yeasts and Molds Causing Food Spoilage, Sciagainst Microb Pathogens: Communicating Current Research and Technological Advances, 1135-1142 (2011).
[36] Sadeghi Mahani F., Aryan far A., Al Sheikh P., “Introduction of Ginger Plant and Its Therapeutic Properties”, 23rd National Congress of Food Science and Technology of Iran, Quchan, Islamic Azad University, Quchan Branch. (2015).
[37] Embuena A.I.C., Nácher M.C., Boix A.C., Pons P.M., Llopis M.B., Martinez M.C.B., Martinez C.G., Quality of Goat′S Milk Cheese as Affected by Coating with Edible Chitosan-Essential Oil Films, Int. J. Dairy Technol., 70: 68-76 (2016).
[38] Pieretti G.G., Pinheiro M.P., Scapim M.R. da S., Mikcha J.M.G., Madrona G.S., Effect of an Edible Alginate Coating With Essential Oil to Improve the Quality of a Fresh Cheese, Acta Scientiarum. Technol, 41(1): e36402 (2019).
[39] Ksouda G., Sellimia S., Merlier F., Falcimaigne-cordin A., Thomasset B., Nasri, M., Hajji, M., Composition, Antibacterial and Antioxidant Activities of Pimpinella Saxifraga Essential Oil and Application to Cheese Preservation as Coating Additive, Food Chem., 288(1): 47-56 (2019).