Optimization Production Conditions on the Amount of Nitrogen Compounds in Iranian Fish Sauce (Mahyaveh)

Document Type : Research Article


Department of Food Science and Technology, Faculty of Agriculture, Varamin-Pishva Branch, Islamic Azad University, Varamin, I.R. IRAN


Mahyaveh is a traditional Iranian fish sauce produced by fermentation and hydrolysis. Fish sauce is considered a rich source of protein and contains essential amino acids. The type of fish, salt concentration, and fermentation time are effective on the nitrogen properties of Iranian fish sauce and its quality. Therefore, the aim of this research is to study the type of fish, the salt concentration, and fermentation time on the nitrogen properties and increase the bioactive compounds of Iranian fish sauce (Mahyaveh). The results are shown that the increase in time (30 to 120 days), and salt concentration (35 to 15%) has a significant effect (P≤0.05) on the increase of nitrogen properties, fermentation time (30 to 120 days) and salt concentration (15 to 35%), but decrease on trimethylamine. Multiple optimizations to achieve the maximum nitrogen properties and minimum trimethylamine in the Mahyaveh with 95.56% desirability is obtained at 120 days of fermentation, the salt concentration of 18.73% with the use of sardine. By optimizing the conditions of production, Mahyaveh can be produced with nutritional value and higher quality.


Main Subjects

[1] AOAC, “Official Methods of Analysis”, 17th ed., Association of Official Analytical Chemists. Washington, DC. (2000).
[2] Achinewhu S.C., Oboh C.A., Chemical, Microbiological and Sensory Properties of Fermented Fish Products from Sardinella Sp. in Nigeria, J. Aqua Food Product Technol., 11(2): 53–59 (2002).
[3] Al-Jedah J.H., Ali M.Z., Robinson R.K., The Inhibitory Action of Spices Against Pathogens that Might Be Capable of Growth in a Fish sauce (Mehiawah) from the Middle East, Int. J. Food Microb., 57(1): 129-133 (2000).  
[4] Angeles Navarrete del Toro M., Fernando García-Carreño L., Evaluation of the Progress of Protein Hydrolysis. Current Protocol Food Analytical Chem., (2004).
[5] Beddows C.G., Ardeshir A.G., Daud W.J.B., Development and Origin of the Volatile Fatty Acids in Budu, J. Sci. Food  Agri., 31(1): 86-92 (2006).
[6] Byun M.W., Lee  ­H., Kim  D.H, Kim  J.H, Yook  H.S., Ahn H.J., Effects of Gamma Radiation on Sensory Qualities, Microbiological and Chemical Properties of Salted and Fermented Squid, J. Food Protect., 63(7): 934-939 (2000).
[7] Capillas C.R., Gillyon C.M., Horner W.F.A., Determination of Volatile Basic Nitrogenand Trimethyleamine Nitrogen in Fish Sauce by Flow Infection Analysis, European Food Research Thecnol., 210(6):434-436 (2000).
[8] Dissaraphong S., Benjakul S., Visessanguan W., Kishimura H., The Influence of Storage Conditions of Tuna on the Chemical, Physical and Microbiological Viscera Before Fermentation Changes in Fish Sauce During Fermentation, Bioresource Technol., 97(16): 2032-2040 (2006).
[9] Hasan Hussein F., Razavi H., Emam Djomeh Z., Evaluation of Physicochemical, Sensorial and Microbiological Attributes of Fermented Camel Sausages, Iran. J. Chem. Chem. Eng. (IJCCE), 38(2): 171-181 (2019).
[10] Jesebel R., Erlinda I., Influence of Salt Concentration on Histamine Formation in Fermented Tuna Viscera (Dayok). Food Sci. & Nutr., 3: 201-206 (2012).
[11] Jiang J., Zeng Q., Zhu Z., Zhang L., Chemical and Sensory Changes Associated Yu-lu Fermentation Process a Traditional Chines Fish Sauce, Food Chem., 104(4): 1629-1634 (2007).
[12] Kilinc B., Cakli S., Tolasa S., Dincer T., Chemical, Microbiological and Sensory Changes Associated with Fish Sauce Processing, J. Europe Food Res. Technol., 222(5): 604-613 (2006).
[13] Kim S., Wijesekara I., Development and Biological Activities of Marine Derived Bioactive Peptides, A Rev: J. Func Foods., 2(1): 1–9 (2010).  
[14] Lopetcharat K., Choi Y.J., Park J.W., Daeschel M.A., Fish Sauce Products and Manufacturing. A Review. Food Rev. Int., 17(1): 65-88 (2001).
[16] Mills S., Stanton C., Hill C., Ross R.P., New Developments and Applications of Bacteriocins And Peptides in Foods, Annual Rev: J. Food Sci. Technol., 2: 299–329 (2011).
[18] Moeini S., Sobhanipour N.F., The Effect Of Thermal Process and Shelf Life on TVN Changes and Peroxide in The Salted Anchovy Sprat, J. Iran. Agri. Sci., 30(4): 771 -781 (1999).
[19] Park J., Fukumoto Y., Fujita E., Tanaka T., Washio T., Otsuka S., Shimizu T., Watanabe K., Abe H., Chemical Composition of Fish Sauces Produced in Southeast and East Asian Countries, J. Food Composit. Analys., 14(2): 113-125 (2001).
[20] Sanceda N.G., Suzuki E., Kurata T., Development of Normal and Branched Chain Volatile Fatty Acids During The Fermentation Process in the Manufacture of Fish Sauce, J. Sci. Food Agri., 81(10): 1013-1018 (2001).
[22] Shih I.L., Guie Chen L., Chang W.T., Wang S.L., Yu T.S., Microbial Reclamation of Fish Processing Wastes for the Production of Fish Sauce, Enzym. Microb. Technol., 33(2-3):154-162 (2003).
[23] Tungkawachara S., Park J.W., Choi Y.J., Biochemical Properties and Consumer Acceptance of Pacific Whiting Fish Sauce. J. Food. Sci., 68(3): 855-860 (2003).
[24] Vioque j., Clemente A., Pedroche J., Yust M.M., Millgn F., Obtencion Yaplicaciones de Hydrolizados Proteicos, J. Grasas Aceites., 52:132-136 (2001). 
[25] Xu W., Yu G., Xue C., Xue Y., Ren Y., Biochemical Changes Associated with Fast Fermentation of Squid Processing by-Products for Low Salt Fish Sauce, Food Chem., 107(4): 1597-1604 (2008).  
[26] Yuen S.K., Yee C.F., Anton A., Microbial Characterization of Budu, an Indigenous Malaysian Fish Sauce, Borneo Sci., 24:24-35 (2009).
[27] Zarei M., Najafzadeh H., Eskandari M.H., Pashmforoush M., Enayati A., Gharibi D., Fazlara A., Chemical and Microbial Properties of Mahyaveh, a Traditional Iranian Fish Sauce, Food Cont., 23(2): 511-514 (2012).