Carotenoid Production by Rhodotorula sp. on Fruit Waste Extract As a Sole Carbon Source and Optimization of Key Parameters

Document Type : Research Article

Authors

Department of Chemical Engineering, National Institute of Technology, Rourkela–769 008, Orissa, INDIA

Abstract

Fruit waste extract was selected as the sole substrate for carotenoid production and the effect of parameters like pH, temperature and agitation have been studied. A two-step simple sequential strategy was employed for the optimization of carotenoid production by Rhodotorula rubra. In the first step, one factor at a time was employed to evaluate the impact of pH on carotenoid production. The outcome revealed that pH has noteworthy influence on pigment production at ambient conditions with constant temperature and agitation. The produced carotenoid was characterized and confirmed by UV-Visible and FT-IR spectroscopic analyses.A Box–Behnken design was then applied in the second step to optimize the pH, temperature and agitation to obtain high pigment yield. The statistical experimental design predicted the high yield conditions of different responses. The interaction between pH and temperature stood vital for improved carotenoid production (2.98 ± 0.28 mg/L) with biomass yield of 7.83 mg/mL by the optimization of significant parameters. The optimum conditions followed for high yield carotenoids are pH (7.0), temperature (28.2 °C), and agitation (150 rpm). Along with the above, biomass growth conditions were also studied and optimum parameter values were reported. The present work shows the effectiveness of abundantly available cheaper fruit waste extract as a sole substrate in obtaining carotenoids in significant amount by R. rubra.

Keywords

Main Subjects

References

[1] Das A., Yoon S.H., Lee S.H., Kim J.Y., Oh D.K., Kim S.W., An Update on Microbial Carotenoid Production: Application of Recent Metabolic Engineering Tools, Applied Microbiology and Biotechnology, 77 (3): 505-512 (2007).
[2] Dufossé L., Galaup P., Yaron A., Arad S.M., Blanc P., Chidambara Murthy K.N., Ravishankar G.A., Microorganisms and Microalgae as Sources of Pigments for Food use: a Scientific Oddity or an Industrial Reality?, Trends in Food Science & Technology, 16(9): 389-406 (2005). doi:http://dx.doi.org/10.1016/j.tifs.2005.02.006
[3] Simova E.D., Frengova G.I., Beshkova D.M., Effect of Aeration on the Production of Carotenoid Pigments by Rhodotorula rubra-lactobacillus casei Subsp. Casei Co-Cultures in Whey Ultrafiltrate. Zeitschrift fur Naturforschung, C-Journal of Biosciences, 58 (3-4):225-229 (2003). http://www.znaturforsch.com/ac/v58c/s58c0225.pdf
[4] Marova I., Carnecka M., Halienova A., Certik M., Dvorakova T., Haronikova A., Use of Several Waste Substrates for Carotenoid-Rich Yeast Biomass Production, Journal of Environmental Management 95,Supplement (0):S338-S342 (2012).
doi:http://dx.doi.org/10.1016/j.jenvman.2011.06.018
[5] Park P., Kim E., Chu K., Chemical Disruption of Yeast Cells for the Isolation of Carotenoid Pigments, Separation and Purification Technology, 53 (2): 148-152 (2007).
[6] Park P., Cho D., Kim E., Chu K., Optimization of Carotenoid Production by Rhodotorula glutinis Using statistical Experimental Design, World Journal of Microbiology and Biotechnology, 21(4): 429-434 (2005). 
[7]  Cabral M.M.S., Cence K., Zeni J., Tsai S.M., Durrer A., Foltran L.L., Toniazzo G., Valduga E., Treichel H., Carotenoids Production from a Newly Isolated Sporidiobolus pararoseus Strain by Submerged Fermentation, European Food Research and Technology, 233 (1): 159-166 (2011).
[8] Ribeiro B., Barreto D., Coelho M., Technological Aspects of β-Carotene Production, Food Bioprocess Technol, 4 (5): 693-701. doi:10.1007/s11947-011-0545-3 (2011).
[9] Nelis H.J., De Leenheer A., Microbial Sources of Carotenoid Pigments Used in Foods and Feeds, Journal of Applied Microbiology, 70 (3):181-191 (1991).       
[10] Liaaen-Jensen S., Andrewes A., Microbial Carotenoids, Annual Reviews in Microbiology, 26(1): 225-248 (1972).
[11] Frengova G.I., Beshkova D.M., Carotenoids from Rhodotorula and Phaffia: Yeasts of Biotechnological Importance, Journal of Industrial Microbiology & Biotechnology, 36 (2):163-180 (2009).
[12] Dung B.D.H., Huyen T.T.B., Quan B.H., Optimization Carotenoids Production by Rhodotorula glutinis GBDOUusing Design of Plackett-Burman Matrix and Central Composite Design-Response Surface Methodology, In: "Conference Proceedings of Biotechnology for Green Solutions and Sustainable Environment", p 25 (2010).
[13] Tinoi J., Rakariyatham N., Deming R., Simplex Optimization of Carotenoid Production by Rhodotorula glutinis Using Hydrolyzed Mung Bean Waste Flour as Substrate, Process Biochemistry, 40(7): 2551-2557 (2005).
[14] Latha B., Jeevaratnam K., Purification and Characterization of the Pigments from Rhodotorula glutinis DFR-PDY Isolated from Natural Source, Global J Biotech Biochem, 5 (3):166-174 (2010).
[15] Bhosale P., Gadre R., Optimization of Carotenoid Production from Hyper‐Producing Rhodotorula glutinis Mutant 32 by a Factorial Approach, Letters in Applied Microbiology, 33 (1):12-16 (2001).
[16] Malisorn C., Suntornsuk W., Optimization of β-Carotene Production by Rhodotorula glutinis DM28 in Fermented Radish Brine, Bioresource Technology, 99 (7): 2281-2287 (2008). doi:http://dx.doi.org/10.1016/j.biortech.2007.05.019
[17] Buzzini P., An Optimization Study of Carotenoid Production by Rhodotorula glutinis DBVPG 3853 from Substrates Containing Concentrated Rectified Grape Must as the Sole Carbohydrate Source, Journal of Industrial Microbiology and Biotechnology, 24 (1): 41-45 (2000).
[18] Surwase S.N., Jadhav S.B., Phugare S.S., Jadhav J.P., Optimization of Melanin Production by Brevundimonas sp. SGJ Using Response Surface Methodology. Biotech, 3: 1-8 (2012).
[19] Thimmaiah S.K., "Standard Methods of Biochemical Analysis", Kalyani publishers (2006).
[20] Coates J., Interpretation of Infrared Spectra, a Practical Approach, "Encyclopedia of Analytical Chemistry" (2000).
Iranian Journal of Chemistry and Chemical Engineering
Volume 33, Issue 3 - Serial Number 71
September 2014
Pages 89-99

Files

Share

How to cite

Statistics