Effective Solvent Systems for Separation of PTH-Amino Acids on Polyamide Sheet

Document Type : Research Article


HEJ Research Institute of Chemistry, University of Karachi, Karachi-75270, PAKISTAN



Two dimensional thin layer chromatography on polyamide sheets is a useful method for identification of PTH-amino acids which have very close Rf-values. The solvent systems described here are particularly effective and have an edge over other solvent systems used for the separation of PTH-amino acids. The solvent systems provide a clear separation of all PTH-amono acids except lle / Leu in a relatively short time.


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