Institute of Chemistry, Mazandaran University, P.O. Box 311, Babolsar, I.R. IRAN
10.30492/ijcce.1999.10067
Abstract
Effects of ATP and Mg2+ concentrations on bioluminescence reaction of luciferase (Photinus pyralis) were investigated by home-made luminometer. The Michaelis constants of the enzyme for ATP and Mg2+ obtained from the Lineweaver-Burk graph, were 61.9 mM ±3.3 mM and 251.6mM ± 39.0mM respectively. Optimum concentration of Mg2+ for maximum luminescence was 0.004 M.
Hadjmohammadi, M. R., & Chaichi, M. J. (1999). Measurement of Michaelis Constants for ATP and Mg2+ in Bioluminescence Reaction of Luciferase by a Home-Made Luminometer. Iranian Journal of Chemistry and Chemical Engineering, 18(2), 83-87. doi: 10.30492/ijcce.1999.10067
MLA
Mohammad Reza Hadjmohammadi; Mohammad Javad Chaichi. "Measurement of Michaelis Constants for ATP and Mg2+ in Bioluminescence Reaction of Luciferase by a Home-Made Luminometer". Iranian Journal of Chemistry and Chemical Engineering, 18, 2, 1999, 83-87. doi: 10.30492/ijcce.1999.10067
HARVARD
Hadjmohammadi, M. R., Chaichi, M. J. (1999). 'Measurement of Michaelis Constants for ATP and Mg2+ in Bioluminescence Reaction of Luciferase by a Home-Made Luminometer', Iranian Journal of Chemistry and Chemical Engineering, 18(2), pp. 83-87. doi: 10.30492/ijcce.1999.10067
VANCOUVER
Hadjmohammadi, M. R., Chaichi, M. J. Measurement of Michaelis Constants for ATP and Mg2+ in Bioluminescence Reaction of Luciferase by a Home-Made Luminometer. Iranian Journal of Chemistry and Chemical Engineering, 1999; 18(2): 83-87. doi: 10.30492/ijcce.1999.10067
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