Application of the Taguchi Design for Production of Poly(β-hydroxybutyrate) by Ralstonia eutropha

Document Type: Research Article

Authors

Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University P.O. Box 14115-143, Tehran, I.R. IRAN

Abstract

The Taguchi design of experiments was used to test the relative importance of medium components and environmental factors on poly(β-hydroxybutyrate)(PHB) production by Ralstonia eutropha. The optimum condition was obtained as: fructose concentration, 15 g/L; C/N ratio, 7.4; agitation speed 200 rpm; culture time, 40 h; temperature, 25 ° C; seed age, 15 h. At optimum condition the yield of PHB production was found to be 92.36%.

Keywords

Main Subjects


[1] Anderson, A. J., Dawes E. A., Occurrence, metabolism, metabolic role, and industrial uses of bacterial polyhydroxyalkanoates. Microbiol Rev. 54, 450 (1990).
[2] Shah-hosseini, S., A fed-batch model for PHA production using Alcaligenes eutrophus. MSc Thesis, University of Queensland, Queesland, Australia (1995).
[3] Howells E., Opportunities in biotechnology for the chemical industry, Chem. Ind., 7, 5008 (1982).
[4] Byrom, D., Polymer synthesis by microorganisms: technology and economics. Trend Biotech. 5, 246(1987).
[5] Poirier, M., Production of polyhydroxybutyrate in transgenic plants, Trend Biotech, 256, 520 (1992).
[6] Logothetis,N., and Wynn, H. P., In “Quality through design: Experimental design, off line quality control and Taguchi’s contribution”, Clarendon press. Oxford, (1989).
[7] Roy, K. R. In “A primer on the Taguchi method” Van nostrand Reinhold, New York, (1990).
[8] Fatemi, S. S. A. and Shojaosadati S. A., Optimization of citric acid production by using experimental design in solid state fermentation by Aspergillus niger, Amirkabir J of Sci. Techno., 11, 314 (2000).
[9] Shojaosadati, S. A. and Babaeipour, V., Citric acid production from apple pomace in multilayer packed bed solid state bioreactor, Process Biochem, 37, 909(2000).
[10] Schlegel, H., Gottschalk, G., Von Bartha, R., Formation and utilization of poly-β-hydroxybutyrate by knallges bacteria (Hydrogenomonas), Nature, 191, 143(1961).
[11] Bradford, D. Growth and accumulation of poly-β-hydroxybutyrate by Alcaligenesis eutrophus. Honours Thesis, Department of Chemical Engineering, University of Queensland (1992).
[12] Braunegg, G., Sonnoleitner, B. and Lafferty, R. M., A rapid gas chromatographic method for the determination of poly-b-hydroxybutyric acid in microbial biomass, Eur J Appl Microbiol Biotech., 6, 29 (1978).
[13] Brandl, H., Gross, R. A., Lenz, R. W., Fuller, R.C. Pseudomonas oleovorans as a source of Poly (β- hydroxyalkanoates) for potential applications as biodegradable polyesters, Appl. Environ.Microbiol., 54, 1977(1988).
[14] Tabandeh, F. and Vasheghani, V., Biosynthesis of Poly(3-hydroxy butytyrate) as an biodegradable polymer, Iranian J. Pol. 12, 37 (2003).
[15] Hejazi, P., Vasheghani, E. and Yamini, Y., Supercritical Disruption of  Ralstonia eutropha for recovery of poly(hydroxybutyrate), Biotechnol. Prog,  (2003) In Press.
[16] Davies,L., In “Efficiency in research, development, and production: The statistical design and analysis of chemical experiments” The Royal Society of Chemistry, Cambridge, (1993).
[17] Haaland, P. D., In “Experimental design in biotechnology”, Elsevier, New York, 85, 16 (1989).