Separation of Biojenic Amines Using Dansyl Cloride Derivatization and Mixed Micellar Liquid Chromatography

Document Type : Research Article

Authors

Department of Chemistry, University of Mazandaran, P.O. Box 453, Babolsar, I.R. IRAN

Abstract

Separation of some biogenic amines via RP-HPLC using mixed micellar mobile phase was investigated. The compounds were derivatized before hand by dansyl chloride as a chromophoric reagent. Appropriate conditions for separation, were determined by studying factors such as temperature, type and percentage of organic modifier, concentration of surfactants (SDS and Brij-35) and the pH of the mobile phase. The number of theoretical plates (N), asymmetry factor (B/A) and selectivity factor (α) considered as criteria for finding the appropriate conditions for separation. Appropriate condition, were: 40˚C, 40 mM SDS, 0.5 mM Brij-35 and 10% 1-propanol at pH=5.

Keywords

Main Subjects


[1] Moret, S., Conte, L. S., J. Chromatogr. A, 729, 363 (1996).
[2] Slomkowska, A., Ambroziak, W., Eur. Food Res. Technol., 215, 360 (2002).
[3] Butehorn, U., Pyell, U., J. Chromatogr. A, 772, 27 (1997).
[4] Voget, M. N., Etenmiller, R. R., Kowhlerand P. K., Handy, M.K., J. Milk Food Technol., 37, 377 (1973).
[5] Butehorn, U., Pyell, U., J. Chromatogr. A, 792, 157 (1997).
[6] Artunyanart, M., Cline Love, L.J., Anal. Chem., 56, 1557 (1984).
[7] Hirofumi Tani, Tamio Kamidate, Hiroto Watanabe, J. Chromatogr. A, 780, 229 (1997).
[8] Armstrong, D. W., Cline Love, L. J., Anal.Chem., 53, 1662 (1984).
[9] Dorsey, J. G., Deechegaray, M. T., Landy, J. S.¸ Anal. Chem., 55, 924 (1963).
[10] Rodgers, A. H., Khaledi, M. G., Anal. Chem., 66, 327 (1994).
[11] HadjMohammadi, M. R., Safa, F., Int. J. Chem., 10 (2), 109 (2002).
[12] HadjMohammadi, M. R., Fatemi, M. H., J. Liq. Chromatogr., 18, 2569 (1995).
[13] HadjMohammadi, M. R., Norouzpour Barsemnani, A., Int. J. Chem., 12 (4), 269 (2002).
[14] Horvath, C., Melander, W. R., Anal. Chem., 49, 2295 (1977).
[15] Borgerding, M. G., Quina, F. H., Hinze,  W. L., Bowermaster, J., Mcnair, H. M., Anal. Chem., 60, 2520 (1988).
[16] Khaledi, M. J., Strasters, J. K., Rodgers, A. H., Breyer, E. D., Anal. Chem., 62, 130 (1990).
[17] Torres-Lapasio, J. R., Vallanueva-Camanas, R. M., Sanchis Mallols, J. M., Medina-Hernandez M.J., Garcia-Alvarez-Coque, M. C., Chromatogr., 639, 87 (1993).
[18] Haginaka, J., Wakai, J., Yasuda, H., J. Chromatogr.488, 341 (1989).
[19] Borgerding, M.G., Hinze, W. L., Anal. Chem., 61, 1353 (1989).