Effect of Temperature and pH on the Growth Kinetics and Carotenoid Production by Sporobolomyces ruberrimus H110 Using Technical Glycerol as Carbon Source

Document Type: Research Note

Authors

1 Departement of Food Science & Engineering, Faculty of Agricultural Biosystem Engineering, University of Tehran, Karaj, I.R. IRAN

2 Laboratoire des Science du Genie Chimique, U.P.R. 6811-C.N.R.S. / I.N.P.L., 13 rue du bois de la champelle, 54500 Vandoeuvre-Les-Nancy, FRANCE

Abstract

A new isolated strain of Sporobolomyces ruberrimus H110 was cultivated  on technical glycerol as carbon source and effect of different temperatures and pH on the growth and pigmentation was studied. The maximum concentration of total carotenoid was 3.84 mgg-1 including torularhodin (3.70 mgg-1) and β-carotene (0.14 mgg-1) using 19ºC at pH 6, but the highest amount of the maximum specific growth rate was obtained (µmax = 0.094 h-1) at 27ºC. The growth yield (Yx/s ) was equal to 0.52 ± 0.01 g g-1 with a correlation coefficient value of 0.98.

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[1] Johnson, E.A. and Schrooder, W.A., In advances in Biochemical Engineering Biotechnology, 53, 119 (1995).
[2] Sandmann, G., Albrecht, M., Schnurr, G., Knörzer, O. and Böger, P., Trends in Biotechnol., 17, 233 (1999).
[3] Nelis, H. J. and  De Leenheer, A. P., J.  Appl. Bacteriol., 70, 181 (1991).
[4] Edge,  R., Mc Garvey, D.J. and Truscott, T.G., J. Photochem. Photobiol. B: Biol., 41,189 (1997).
[5] Young, A.J., Lowe, G.M. and Vlismas, K., Mol. Aspects Med., 24, 363 (2003).  
[6] Bhosale, P.B. and Gadre, R.V., Appl. Microbiol. Biotechnol., 55, 423 (2001).
[7] Fregova, G., Simova, E., Pavlova, K., Beshkova, D. and Grogora, D., Biotechnol.  Bioeng., 44, 888 (1994).
[8] Meyer, P.S. and Du Preez, J.C., System. Appl. Microbiol., 17, 24 (1994).
[9] Schroeder, W.A. and Johnson, E. A., J. Gen. Microbiol., 139, 907 (1993).
[10] Schroeder,  W. A.  and Johnson,  E. A.,  J. Ind. Microbiol., 14, 502 (1995).
[11] Girad, P., Falconnier, B., Bricout, J. and Vladesc, B., Appl. Microbiol. Biotechnol., 41, 183 (1994).
[12] An, G. H., Schuman, D.B. and Johnson, E.A., Appl. Environ. Microbiol., 55, 116 (1989).
[13] Kusdiyantini, E., Gaudin, P., Goma, G. and Blanc, P.G., Biotechnol. Lett., 20, 929 (1998).
[14] Sakaki, H., Nochide, H., Komemushi, S. and Miki, W., J. Biosci. Bioeng, 93, 338 (2002)..
[15] Ershov,  Y.U., Dmitrovsky, A.A., Poloyokh, O.V., Podoprigora, O.I. and Bykhovsky V.Y., Prikl. Biokhim. Mikrobiol., 28, 680 (1992).
[16] Eugiena, M., Talos, D., Panaitescu, Contrea. A., Trif, A., Caprita, R., Bogdan, G.H., Gravila, C., Manu, C., Driha, R., Coman, M. and Marinovici, A.V., Rom. Biotechnology Letters, 2, 55 (1997).