Hosseini, M., Bahmani, B., Moztarzadeh, F., Rabiee, M. (2008). Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer. Iranian Journal of Chemistry and Chemical Engineering (IJCCE), 27(1), 115-121.
Mohammad Hosseini; Baharak Bahmani; Fatollah Moztarzadeh; Mohammad Rabiee. "Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer". Iranian Journal of Chemistry and Chemical Engineering (IJCCE), 27, 1, 2008, 115-121.
Hosseini, M., Bahmani, B., Moztarzadeh, F., Rabiee, M. (2008). 'Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer', Iranian Journal of Chemistry and Chemical Engineering (IJCCE), 27(1), pp. 115-121.
Hosseini, M., Bahmani, B., Moztarzadeh, F., Rabiee, M. Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer. Iranian Journal of Chemistry and Chemical Engineering (IJCCE), 2008; 27(1): 115-121.
Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer
1Department of Chemical Engineering, Amirkabir University of Technology, P.O. Box 15874-4413 Tehran, I.R. IRAN
2Department of Biomedical Engineering, Amirkabir University of Technology, P.O. Box 15875-4413 Tehran, I.R. IRAN
Abstract
In this research, an enzyme modified electrode has been produced during the electropolymerization of aniline through incorporation of sulfite oxidase into a conducting polymer. Then the bioelectrochemical response of resulted sulfite biosensor was investigated at different experimental conditions. Study of the stability of the resulted sulfite biosensor revealed that formation of a passive film on the aluminum surface causes improved stability of the electroactive films formed on the electrode surface. The bioelectrochemical response of the enzyme-modified electrode as a sulfite biosensor was investigated at different experimental conditions. The optimum pH and temperature were 8.5 and 35°C, respectively. The apparent Michaelis-Menten constant and the activation energy of the enzyme catalyzed reaction were calculated.