Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer

Hosseini, Mohammad; Bahmani, Baharak; Moztarzadeh, Fatollah; Rabiee, Mohammad

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Iranian Journal of Chemistry and Chemical Engineering (IJCCE)

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	Fabrication of a Sulfite Biosensor by the Use of Conducting Polymer
Article 13, Volume 27, Issue 1, March and April 2008, Page 115-121 PDF (184 K)
Document Type: Research Article
Authors
Mohammad Hosseini ¹; Baharak Bahmani²; Fatollah Moztarzadeh²; Mohammad Rabiee²
¹Department of Chemical Engineering, Amirkabir University of Technology, P.O. Box 15874-4413 Tehran, I.R. IRAN
²Department of Biomedical Engineering, Amirkabir University of Technology, P.O. Box 15875-4413 Tehran, I.R. IRAN
Abstract
In this research, an enzyme modified electrode has been produced during the electropolymerization of aniline through incorporation of sulfite oxidase into a conducting polymer. Then the bioelectrochemical response of resulted sulfite biosensor was investigated at different experimental conditions. Study of the stability of the resulted sulfite biosensor revealed that formation of a passive film on the aluminum surface causes improved stability of the electroactive films formed on the electrode surface. The bioelectrochemical response of the enzyme-modified electrode as a sulfite biosensor was investigated at different experimental conditions. The optimum pH and temperature were 8.5 and 35°C, respectively. The apparent Michaelis-Menten constant and the activation energy of the enzyme catalyzed reaction were calculated.
Keywords
Sulfite biosensor; Aniline; Sulfite oxidase; Conducting polymer
Main Subjects
Biotechnology; Polymers & Composites


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