Application of Plackett Burman Design for Citric Acid Production from Pretreated and Untreated Wheat Straw

Document Type : Research Article

Authors

1 Department of Food Technology Research, National Nutrition and Food Technology Research Institute, Shaheed Beheshti University, M.C., P.O. Box 19395 Tehran, I.R. IRAN

2 Department of Chemical Engineering, Faculty of Engineering, Azad Islamic University, Science and Technology Campus, P.O. Box 14115-775 Tehran, I.R. IRAN

3 Department of Bioprocess Engineering, National Institute for Genetic Engineering and Biotechnology, P.O. Box 14965-161 Tehran, I.R. IRAN

Abstract

A solid state fermentation method was used to utilize wheat straw as substrates for citric acid production by using Aspergillus niger ATCC 9142. The Plackett Burman design (PBD) of experiments was used to test the relative importance of the variables affecting production such as moisture content, age of spore, inoculum size, initial pH of substrate, methanol concentration, incubation temperature and time, as well as initial sugar concentration, steam time and type of solvent. In best condition, the microorganism produced 47 g citric acid per kg dry crude wheat straw, with yield of 93 % based on the amount of fermentable sugar consumed. The effect of pretreatment of wheat straw with HCl, NaOH and urea on the yield of production was also investigated by using PBD. Acid, alkaline and urea pretreatment of wheat straw increased citric acid concentration to 115, 85.0 and 109.5 g/kg of dry wheat straw and yield to 97, 97 and 96 % (respectively) based on available sugar consumed. Finally, up scaling was achieved to a 20-L solid state fermentor (tray bioreactor) in which moisture was constant in gas phase and acid pretreated wheat straw was selected as the most efficient pretreated substrate. The produced acid concentration and yield in fermentor was 60.53 g/kg of dry wheat straw and 2.11 g/kg.day, respectively.  

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References

[1] Amartey, S.A., Leung, P.C.J., Baghaei-Yazdi, N., Leak, D.J., Hartley, B.S., Fermentation of a wheat Straw Acid Hydrolysate by Bacillus T-13 in Continues Culture with Stearothermophilus Partial Cell Recycle, Process Biochem., 34, 289 (1999).
[2] Al-Obaidi, Z. and Berry, D.R., The Use of Deionised Date Syrup as a Substrate for Citric Acid Fer-mentation, Bitechnol. Lett., 1, 153 (1979).
[3] Assadi, M.M. and Nikkhah, M., Production of Citric Acid from Date Pulp by SolidState Fermentation, J. Agricul. Sci. Technol., 4, 119(2002).
[4] Hang, Y.D. and Woodams, E.E., Microbial Pro-duction of Citric Acid by Solid-State Fermentation of Kiwifruit Peel, J. Food Sci., 52, 226 (1987).
[5] Hang, Y.D. and Woodams, E.E., Utilization of Grape Pomace for Citric Acid Production by Solid-State Fermentation, Am. J. Enol. Vitic., 37, 141 (1986).
[6] Hang, Y.D. and Woodams, E.E., Solid State Fermentation of Apple Pomace for Citric Acid Production, MIRCEN J., 2, 283 (1986).
[7] Fatemi, S.S. and Shojaosadati, S.A., Citric Acid Production from Apple Pomace in SolidState Fermentation, Iran. J. Chem. Chem. Eng., 18, 44(1999).
[8] Fatemi, S.S. and Shojaosadati, S.A., Experimental Design Optimisation of Citric Acid Production by Aspergillu niger in Solid State Fermentation. Amirkabir J., 11, 314 (2001).
[9] Shojaosadati, S.A. and Babaeipour, V., Citric Acid Production from Apple Pomace in Multi-Layer Packed Bed Solid-State Bioreactor, ProcessBiochem., 37, 909 (2002).
[10] Tran, C.T. and Mitchell, D.A., Pine Apple Waste-A Novel Substrate for Citric Acid Production by Solid- State Fermentation, Biotechnol.Lett., 17, 1107 (1995).
[11] Kumar, D., Jain, V.K., Shanker, G., Srivastav, A., Utilisation of Fruits Waste for Citric Acid Production by Solid State Fermentation, Process Biochem., 38, 1725 (2003).
[12] Kumagai, K., Usami, S., Hattori, S., Citric Acid Production from Mandarin Orange Waste by Solid Culture of Aspergillus niger., Hakkokogaku., 59, 461 (1981).
[13] Roukas, T. and Kotzekidou, P., Production of Citric Acid from Brewery Wastes by Surface Fermentation Using Aspergillus niger,J.Food Sci., 51, 225 (1986).
[14] Hang, Y.D. and Woodams, E.E., Production of Citric Acid from Corncobs by Aspergillus niger, Bioresour. Technol., 65, 251 (1998).
[15] Shankaranand, V.S. and Lonsane, B.K., Coffee Husk: An Inexpensive Substrate for Production of Citric Acid by Aspergillus niger in a Solid-State Fer-mentation System, WorldJ.Microbiol.Biotechnol., 10, 165 (1994).
[16] Lu,  M.,  Maddox, IS., Brook, J.D., Citric  Aid Production by Solid State Fermentation in a Packed Bed Reactor Using, Aspergillus niger, Bioresour. Technol., 54, 235 (1995).
[17] Khare, S.K., Krishana, J., Gandhi, A.P., Citric Acid Production from Okara (soy residue) by Solid State Fermentation, Bioresour. Technol., 54, 323 (1995).
[18] Haq, I.U., Ali, S., Iqbal, J., Direct Production of Citric Acid from Raw Starch by Aspergillus niger, Process Biochem., 38, 921 (2003).
[19] Luciana, P., Vandenberghe, S., Soccol, C.R., Pandey, A., Lebeault, J.M., Solid-State Fermentation for the Synthesis of Citric Acid by Aspergillus niger, Bioresour. Technol., 74, 175 (2000).
[20] Alben, E. and Erkmen, O., Production of Citric Acid from a New Substrate, Undersized Semolina, by Aspergillus niger, Food Technol. Biotechnol., 42, 19 (2004).
[21] Roukas, T., Citric Acid Production from Carob Pod by Solid State Fermentation, Enzyme Microbial Technol., 24, 54 (1999).
[22] Yamada, K., Bioengineering Report: Recent Advances in Industrial Fermentation in Japan, Biotechnol. Bioeng., 19, 1563 (1977).
[23] Rohr, M., A Century of Citric Acid Fermentation and Research, Food Technol. Biotechnol.,36, 163 (1998).
[24] Montane, D., Farriol, X., Salvado, J., Jollez, P., Chornet, E., Application of Stream Explosion to the Fractionation and Rapid Vapor-Phase Alkaline Pulping of Wheat Straw, Biomass Bioenergy., 14, 261 (1998).
[25] Saha, B.C., Iten, L.B., Cotta, M.A., Victor, W.Y., Dilute Acid Pretreatment, Enzymatic Saccharifica-tion and Fermentation of Wheat Straw to Ethanol, Process Biochem., 40, 3693 (2005).
[26] Gould, J.M., Studies on the Mechanism of Alkaline Peroxide  Delignification  of  Agricultural  Residues,   Biotechnol. Bioeng., 27, 225 (1985).
[27] Sun, R., Lawther, J.M., Banks, W.B., Fractional and Structural Characterization of Wheat Straw Hemicelluloses, Carbohydr. Polym., 29, 325 (1996).
[28] Sun, X.F., Sun, R.C., Fowler, P., Baird, M.S., Isolation and Characterization of Cellulose Obtained by a Two-Stage Treatment with Organosolv and Cyanamide Activated Hydrogen Peroxide from Wheat Straw, Carbohydr. Res., 55, 379 (2004).
[29] Schmidt,  A.S. and  Thomsen,  A.B., Optimization of Wet Oxidation Pretreatment of Wheat Straw, Bioresour. Technol., 64, 139 (1998).
[30] Kurbanoglu, E.B. and Kurbanoglu, N.L., Production of Citric Acid from Ram Horn Hydrolysate by Aspergillus niger,ProcessBiochem., 38, 1421 (2003).
[31] Haq, I., Khurshid, S., Ali, S., Ashraf, H., Qadeer, M.A., Rajoka, M.I., Mutation of Aspergillus niger for Enhanced Citric Acid Production by Blackstrap Molasses, World J. Microbiol. Biotechnol., 17, 35 (2001).
[32] Manonmani, H.K. and Sreekantiah, K.R., Studies on the Conversion of Cellulose Hydrolysate into Citric Acid by Aspergillus niger, Process Biochem.,1, 92 (1987).
[33] Hesseltine, C.W., Solid-state Fermentations. Bio-technol. Bioeng., 14, 517 (1972).
[34] Pandey, A. and Soccol, C.R., Bioconversion of Biomass: A Case of Lignocellulosics Bioconversions in Solid-State Fermentation, Brazilian Arch. Biol. Technol. 41, 379 (1998).
[35] Kapoor, K.K., Chaudhari, K., Tauro, P. In “Prescott and Dunn's Industrial Microbiology”, AVI Publishing Co Inc, WestPort, (1982).
[36] Gutierrez-Rozas, M., Cordova, J., Auria, R., Revah, S., Favela-Torres, E., Citric Acid and Polyols Production by Aspergillus niger at High Glucose Concentration in Solid State Fermentation on Inert Support, Biotechnol. Lett., 17, 219 (1995).
[37] Lee, Y.Y., Lyer, P., Torget, R.W., Dilute Acid Hydrolysis of Lignocellulosic Biomass, Adv. Biochem. Eng. Biotechnol., 65, 93 (1999).
[38] Saha, B.C. and Bothast, R.J., Pretreatment and Enzymatic Saccharification of Corn Fiber, Appl. Biochem. Biotechnol., 76, 65 (1999).
[39] Saha, B.C., In Lignocellulose Biodegradation and Applications in Biotechnology, AmericanChemical Society, Washington, (2004).
[40] Gonzalez, G., Lopez-Santin, J., Caminal, G., Sola, C., Dilute Acid Hydrolysis of Wheat Straw Hemi-cellulose at Moderate Temperature: A Simplified Kinetic Model, Biotechnol. Bioeng., 28, 288 (1986).
[41] Plackett, R.L. and Burman, J.P., The Design of Optimum Multifactorial Experiments, Biometrika., 33, 305 (1946).
[42] Haaland, P. D., In “Experimental Design in Bio-technology”, Elsevier, New York, (1989).
[43] Monaghan, R.L. and Koupal, L.R., In “Use of the Plackett-Burman Technique in a Discovery Program for New Natural Products: Novel Microbial Products for Medicine and Agriculture”, 1st ed.,Demain, A.L., Somkuti, G.A., Hunter- Cevera, J.C., Rossmoore, H.W., Society for Industrial Micro-biology, (1989).
[44] Strobel, R.J. and Nakatsukasa, W.M., Response Surface Method for Optimizing Saccharopolyspora Spinosa, A Novel Macrolide Producer, J. Ind. Microbiol. 11,121 (1993).
[45] Yu, X., Hallett, S.G., Sheppard, J., Watson, A.K., Application of the Plackett-Burman Experimental Design to Evaluate Nutritional Requirements for the Production of Collototrichum Coccodes Spores, Appl. Microbiol. Biotechnol., 44, 301 (1997).
[46] Ghanem, N. B., Yusef, H. H., Mahrouse, H. K., Production of Aspergillus Terreus Xylanase in Solid-State Cultures: Application of the Plackett-Burman Experimental Design to Evaluate Nutritional Requirements, Bioresour. Biotechnol., 73, 113 (2000).
[47] Khosravi   Darani,   K.   Vasheghani  Farahani,  E., Shojaosadati, S.A., Application of the Plackett-Burman Design for the Optimization of Poly(β-Hydroxybutyrate) Production by Ralstonia Eutropha, Iranian J. Biotechnol., 1, 155 (2003).
[48] Rao, L. and Divakar, S., Lipase Catalyzed Esterification of a-Terpineol with Various Organic Acids: Application of the Plackett-Burman Design. Process Biochem., 36, 1125 (2001).
[49] Torres, N.V., Voit, E.O., Gonzalez-Alcon, C., Rodriguez, F., A Novel Approach for Design an Overexpression Strategy for Metabolic Engineering. Application to the Carbohydrate Metabolism in the Citric Acid Producing Mould Aspergillus niger, Food Technol. Biotechnol., 36, 177 (1998).
[50] Gibbons, W. R. and  Westby, C. A.,  Solid  Phase Fermentation of Fodder Beets for Ethanol Production; Effect of Grid Size, J. Ferment. Technol., 64, 179 (1986).
[51] James, C.S., In “Analytical Chemistry of Foods”, Blackie Academic & Professional, London, (1995).
[52] Harris, L.E., In “Nutrition Research Techniques for Domestic and Wild Animal”, UtahState, Logan University, Utah, (1970).
[53] Byers, M., In “Practical Details for the Small Scale Extraction of Protein from Leaves”, Rothamstead Experimental Station, U.K. (1967).
[54] Marier, J.R. and Boulets, M.A.,Direct Determination of Citric Acid in Milk with an Improved Pyridine Acetic Anhydride Method, J. Dairy Sci., 41, 1683 (1958).
[55] Miller, G.L., Use of Dinitrosalycilic Acid Reagent for Determination of Reducing Sugars, Anal. Chem., 31, 426 (1959).
[56] Davies, L., In “Efficiency in Research, Development, and Production: The Statistical Designand Analysis of Chemical Experiments”, The Royal Society of Chemistry, Cambridge, (1993).
[57] Logothetis, N. and Wynn, H.P., In “Quality through Design: Experimental Design, off Line Quality Control and Taguchi’s Contribution”, Clarendon Press, Oxford, (1989).
[58] Ngadi, M.O. and Correia, L.R., Solid-State Ethanol Fermentation of Apple Pomace as Affected by Miosture and Bioreactor Mixing Speed, J. Food Sci., 57, 667 (1992).
[59] Kargi, F., Curme, J.A., Sheehan, J.J., Solid-State Fermentation of Sweet Sorghum to Ethanol, Biotechnol. Bioeng., 27, 34 (1985).
[60] Shankaranand, V.S. and Lonsane, B.K., Ability of Aspergillus niger to Tolerate Metal Ions and Minerals in Solid State Fermentation System for Production of Citric Acid, Process Biochem., 29, 29 (1994).
[61] Hang, Y.D., Splittstoesser, D.F., Woodams, E.E., Utilization of Brewery Spent Grain Liquor by Aspergillus niger, Appl. Microbiol.,30, 879 (1975).
[62] Hang, Y.D., Splittsteoesser, D.E., Woodams, E.E., Sherman, R.M., Citric Acid Fermentation of Brewery Waste, J. Food Sci., 42, 383 (1977).
[63] Roukas, T. and Kotzekidou, P., Influence of some Trace  Metals and Stimulants on Citric Acid        Production from Brewery Wastes by Aspergillus niger, Enzyme Microb. Technol., 9, 291 (1987).
[64] Maddox, I.S., Hossain, M., Brooks, J.D., The Effect of Methanol on Citric Acid Production from Galactose by Aspergillus niger, Appl. Microbiol. Biotechnol., 23, 203 (1986).
[65] Rohr, M., Kubicek, C. P., Kominek, J.,  In “Bio-technology: Biomass microorganisms for Special Applications, Microbial Products and Energy from Renewable Sources”, Verlag Chemie, Weinheim, (1998).
[66] Hang, Y.D. and Woodams, E.E., A Process for Leaching Citric Acid from Apple Pomace Fermented with Aspergillus niger in Solid-State Culture, MIRCEN J., 5, 379 (1989).
[67] Kristiansen, B. and Sinclair, C.G., Production of Citric Acid in Batch Culture, Biotechnol. Bioeng., 20, 1711 (1978).
[68] Mitchell,  D. A.,  Targonski,  Z.,  Rogalski,  J., Leonowicz, A., In “Solid Substrate Cultivation”, Elsevier, Sci. Publ. Ltd, London and New York, (1992).
[69] Fernandez Vergano, M.G., Soria, M.A., Kerber, N.L., Influence of Inoculum Preparation on Citric Acid Production by Aspergillus niger, World J. Microbiol. Biotechnol., 12, 655 (1996).
[70] Stowe, R.A. and Mayer, R.P., Efficient Screening of Process Variables, Ind. Eng. Chem., 58, 36(1996).
[71] Szewezyk, K.W. and Myszka, L., The Effect of Temperature on the Growth of Aspergillus niger in Solid-State Fermentation, Bioproc. Eng.,10, 123 (1994).
[72] Khosravi Darani, K. and Zoghi, A., Comparison of Pretreatment Strategies of Sugarcane Baggase: Experimental Design for Citric acid Production, Bioresour. Technol., 99(15), 6986 (2008).

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