Green Synthesis of Silver Nanocomposite from Zataria Multiflora Boiss Extract for Antioxidant, Antibacterial and Anticancer Activities

Articles in Press

Document Type : Research Article

Authors

1 Department of Chemistry, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran

2 Department of Pharmacy, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran

Abstract

Development of green synthesis of silver nanocomposites has attracted considerable worldwide attention in matter of medical science and disease treatment. In the present work, silver nanoparticles/ordered mesoporous carbon (AgNPs/OMC) nanocomposite was synthesized in the presence of Zataria multiflora Boiss extract as a reducer and stabilizer agent. The FE-SEM results showed rod shape Ag nanoparticles with the main particle size of 33.6±1.4 nm which is in agreement well with the XRD results (41.83) and the face-central cubic structure on OMC. The average surface concentration (Г) of the Ag onto OMC was obtained (1.07±0.36) × 10-10 mol/cm2. The AgNPs/OMC exhibited excellent antioxidant, antibacterial and anticancer activity. The content of total phenols 12 ppm and DPPH free radical scavenging 81.23% were calculated. The antibacterial activity of AgNPs/OMC nanocomposite was tested on gram-negative (Escherichia coli) and gram-positive (Staphylococcus Aureus) bacterial species. The results exhibited strong antibacterial activity against bacterial species especially Staphylococcus aurous with minimum inhibition concentration (MIC) of 31.25 µg/mL and minimum bactericidal concentration (MBC) of 62.5 µg/mL. Also, in this work, we evaluated cell cytotoxicity effects of AgNPs/OMC against breast cancer (AU145). The cell test experiments (MTT) showed higher viability loss and significant toxicity of AgNPs/OMC nanocomposite (300 µg/mL) against AU145 cells with the cell viability of ~23 % and ~12% at 24 and 48, respectively. The half-maximal inhibitory concentration (IC50) value for AU145 was found significantly low with AgNPs/OMC (in comparison to OMC) due to the presence of rich Ag nanoparticles at the surface of OMC. Thus, the AgNPs/OMC nanocomposite because of unique physicochemical, high surface area and stronger scavenging properties could potentially act as antioxidant, anticancer and antibacterial agent, which may be applied in clinical systems.

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Iranian Journal of Chemistry and Chemical Engineering

Articles in Press, Accepted Manuscript
Available Online from 01 January 2024

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