Selective Simple Solid Phase Extraction Coupled with High-Performance Liquid Chromatographic Determination of Repaglinide by Functionalized SBA-15 Nanoadsorbent

Articles in Press

Document Type : Research Article

Authors

1 Phase separation and FIA Lab., Department of Chemistry, Faculty of Science, University of Zanjan, Zanjan, Iran

2 Phase Separation and FIA Laboratory, Department of Chemistry, Faculty of Science, University of Zanjan, Zanjan, Iran

3 Pharmaceutical Nanotechnology Research Center (ZPNRC), Zanjan University of Medical Sciences, Zanjan, Iran

Abstract

The straightforward solid-phase extraction (SPE) approach coupled with high-performance liquid chromatography (HPLC) was studied for its potential use in the measurement of low-concentration levels of Repaglinide (REP), a common anti-diabetic medicine. In this study, we successfully synthesized nanoparticles of phenyl-functionalized SBA-15 (referred to as SBA-15-Ph) and used them as an intelligent adsorbent to capture REP in certain biological samples. Some characterization and verification methods were used to outline the mesoporous silica nanoparticles; these methods included Fourier transform infrared spectroscopy (FT-IR), small-angle X-ray diffraction (SAXRD), scanning electron microscopy (SEM), and N2 adsorption/desorption studies. The results showed that the phenyl functional groups were well absorbed into the surface of SBA-15 and that the as-made products exhibited hexagonal mesostructures. Significant merit values were also achieved, including a linear range spanning from 2.0 to 500 ng/mL, exhibiting a high correlation coefficient (R2) of 0.9947, and demonstrating a limit of detection as low as 0.68 ng/mL. Repaglinide samples were quickly cleaned up and enriched using the suggested approach for chromatographic analysis, demonstrating the method's efficacy.

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Iranian Journal of Chemistry and Chemical Engineering

Articles in Press, Accepted Manuscript
Available Online from 29 October 2023

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