Influence of Different Nitrogen Sources on Amount of Chitosan Production by Aspergillus niger in Solid State Fermentation

Document Type : Research Article

Authors

1 Biochemical and Bioenvironmental Engineering Research Center, Sharif University of Technology, Tehran, I.R. IRAN

2 Department of Chemical and Petroleum Engineering, Sharif University of Technology, Tehran, I.R. IRAN

Abstract

In this study the effect of different nitrogen source substrates on the amount of chitosan production by Aspergillus niger was investigated. A. niger PTCC 5012 from the Persian Type Culture Collection (PTCC) was grown on soy bean, corn seed and canola residues at 30 °C for specified cultivation days under sterilized conditions. Chitosan was extracted from the fungal mycelia using hot alkaline and acid treatment. The results were shown that soy bean residue at moisture of 37% and 8.40.26 % of nitrogen content produced the highest amount of chitosan (17.0530.95 g/kg of dry substrate), after 12 days of incubation. Corn seed residue produced very low amount of chitosan (1.90.4 % of nitrogen content). The chitosan was analyzed by FTIR spectroscopy and its spectrum was recorded. 
 

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[1] Nwe, N., Stevens, W.F., Effect of Urea on Fungal Chitosan Production in Solid Substrate Fermentation, Process Biochemistry, 39, 1639 (2004).
[2] Nwe, N., Chandrkrachang, S., Stevens, W.F., Maw, T., Tan, T.K., Khor, E., Production of Fungal Chitosan by SolidState and Submerged Fermen-tation, Carbohydrate Polymers, 49, 235 (2002).
[3] Harish Prashanth, K.V., Tharanathan, R.N., Chitin/ Chitosan: Modifications and their Unlimited Application Potential- An Overview, Trends in Food Science & Technology, 17, 1 (2006).
[4] Shahidi, F., Arachchi, J.K.V., Jeon, Y.J., Food Applications of Chitin and Chitosan, Trends in Food Science &Technology, 10, 37 (1999).
[5] Suntornsk, W., Pochanavanich, P., Suntornsk, L., Fungal Chitosan Production on Food Processing by Products, Process Biochemistry, 37, 727 (2002).
[6] Hu, K.J., Hu, J.L., Ho, K.P., Yeung, K.W., Screening of Fungi for Chitosan Producers, and Copper Adsorption Capacity of Fungal Chitosan and Chitosanaceous Materials, Carbohydrate Polymers, 58, 45 (2004).
[7] Guha, A.K., Chatterjee, B.P., Chatterjee, S., Adhya, M., Chitosan from Mucor rouxii: Production and Physico-Chemical Characterization, Process Biochemistry, 40, 395 (2005).
[8] Crestini, C., Kovac, B., Sermanni, G.G., Production and Isolation of Chitosan by Submerged and
Solid-State Fermentation from Lentinus Edodes, Biotechnology and Bioengineering, 50, 207 (1996).
[9] Niederhofer, A., Muller, B.W., A Method for Direct Preparation of Chitosan with Low Molecular Weight from Fungi, European Journal of Pharmaceutics and Biopharmaceutics, 57, 101 (2004).
[10] Bradford, M.M., A Rapid and Sensitive Method for Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein Binding, Anal. Biochem.,72, 248 (1976).
[11] Whistler, R.L., Wolfrom, M.L., Methods in Carbo-hydrate Chemistry, Academic Press, New York and London, 1, (1962).
[12] Atlas, R. M., “Handbook of Microbiological Media”, 2nd Edition, Edited by Parks, L. C., Academic Press, (1996).
[13] Nyein Aye, K., Karuppuswamy, R., Tangir Ahamed, Stevens, W.F., Peripheral Enzymatic Deacetylation of Chitin and Reprecipitated Chitin Particles, Bioresource Technology, 97, 577 (2006).
[14] Rodriguez  Couto,  S.,  Angeles  Sanroman,  M., Application of Solid-State Fermentation to Food Industry- A Review, Journal of Food Engineering, 76, 291 (2006).
[15] Viccini,  G.,  et al.,  Analysis  of  Growth  Kinetic Profiles in SSF, Food Technol. Biotechnol, 39, A-B (2001).