• Home
  • Browse
    • Current Issue
    • By Issue
    • By Author
    • By Subject
    • Author Index
    • Keyword Index
  • Journal Info
    • About Journal
    • Aims and Scope
    • Editorial Board
    • Editorial Staff
    • Publication Ethics
    • Indexing and Abstracting
    • Related Links
    • FAQ
    • Peer Review Process
    • News
  • Guide for Authors
  • Submit Manuscript
  • Reviewers
  • Contact Us
 
  • Login
  • Register
Home Articles List Article Information
  • Save Records
  • |
  • Printable Version
  • |
  • Recommend
  • |
  • How to cite Export to
    RIS EndNote BibTeX APA MLA Harvard Vancouver
  • |
  • Share Share
    CiteULike Mendeley Facebook Google LinkedIn Twitter Telegram
Iranian Journal of Chemistry and Chemical Engineering (IJCCE)
Articles in Press
Current Issue
Journal Archive
Volume Volume 36 (2017)
Volume Volume 35 (2016)
Volume Volume 34 (2015)
Volume Volume 33 (2014)
Volume Volume 32 (2013)
Volume Volume 31 (2012)
Volume Volume 30 (2011)
Volume Volume 29 (2010)
Volume Volume 28 (2009)
Volume Volume 27 (2008)
Issue Issue 4
Issue Issue 3
Issue Issue 2
Issue Issue 1
Volume Volume 26 (2007)
Volume Volume 25 (2006)
Volume Volume 24 (2005)
Volume Volume 23 (2004)
Volume Volume 22 (2003)
Volume Volume 21 (2002)
Volume Volume 20 (2001)
Volume Volume 19 (2000)
Volume Volume 18 (1999)
Volume Volume 17 (1998)
Volume Volume 16 (1997)
Volume Volume 15 (1996)
Volume Volume 14 (1995)
Volume Volume 13 (1994)
Volume Volume 12 (1993)
Volume Volume 11 (1992)
Volume Volume 10 (1991)
Volume Volume 9 (1990)
Volume Volume 8 (1989)
Volume Volume 7 (1988)
Volume Volume 6 (1987)
Mohamed Salleh, M., Jamal, P., Farouk Gad, A., Has-Yun Hashim, Y., Hasim, H. (2008). Comparison of Alcohol Free GSH Production by Ultrasonic and Homogenizing Method. Iranian Journal of Chemistry and Chemical Engineering (IJCCE), 27(4), 107-113.
Mohamad Ramlan Mohamed Salleh; Parveen Jamal; Abd. Elaziem Farouk Gad; Yumi Zuhanis Has-Yun Hashim; Haslinda Hasim. "Comparison of Alcohol Free GSH Production by Ultrasonic and Homogenizing Method". Iranian Journal of Chemistry and Chemical Engineering (IJCCE), 27, 4, 2008, 107-113.
Mohamed Salleh, M., Jamal, P., Farouk Gad, A., Has-Yun Hashim, Y., Hasim, H. (2008). 'Comparison of Alcohol Free GSH Production by Ultrasonic and Homogenizing Method', Iranian Journal of Chemistry and Chemical Engineering (IJCCE), 27(4), pp. 107-113.
Mohamed Salleh, M., Jamal, P., Farouk Gad, A., Has-Yun Hashim, Y., Hasim, H. Comparison of Alcohol Free GSH Production by Ultrasonic and Homogenizing Method. Iranian Journal of Chemistry and Chemical Engineering (IJCCE), 2008; 27(4): 107-113.

Comparison of Alcohol Free GSH Production by Ultrasonic and Homogenizing Method

Article 14, Volume 27, Issue 4, November and December 2008, Page 107-113  XML PDF (163 K)
Document Type: Research Note
Authors
Mohamad Ramlan Mohamed Salleh ; Parveen Jamal; Abd. Elaziem Farouk Gad; Yumi Zuhanis Has-Yun Hashim; Haslinda Hasim
Department of Biotechnology Engineering, Faculty of Engineering, International Islamic University Malaysia, Jalan Gombak 53100, Kuala Lumpur, MALAYSIA
Abstract
Homogenizer and ultrasonicator that we call as mechanical methods were used in the lab scale production of alcohol free reduced glutathione (alcohol free GSH) from baker’s yeast. The study was done to determine the effects of specific parameters of the methods and the pH of the medium on the production of the GSH. The cell disruptions were run at a wide range of those parameters at the suitable common parameters of the methods. The disrupted cells suspension was then centrifuged at 12,000 rpm for 20 min and the GSH content in the supernatant was analyzed by taking the absorbance (A) value of the solution by spectrophotometer at 412 nm. The results obtained showed that the production of GSH was affected by the adjusted specific parameters of each method and the yield of production by the homogenizing and ultrasonic method was found 3.3 and 4.1 times, respectively, better than the yield of production by the unadjusted parameters. Ultrasonic method which produced 23.2 mg/L product was found 1.3 times better than the homogenizing method (18.5 mg/L) in producing the GSH. The result obtained from the lab scale study of the above mentioned mechanical methods showed that the implementation of them in the large scale production of the alcohol free GSH is possible. 
Keywords
Alcohol free GSH; Ultrasonic method; Homogenizing method; Baker’s yeast; Cells disruption; Specific parameters
Main Subjects
Food Science & Technology; Process Design, Simulation & Control
References
1] Stephen, D.W., Jamieson, D.J., Glutathione is an Important Antioxidant Molecule in the Yeast Saccharomyces cerevisiae, FEMS. Microbiol. Lett., 141, 207(1996).

[2] Li, Y., Chen, J., Song, Q., Katakura, Y., Lun, S.Y., Fed-Batch Culture Strategy for High Yield of Baker's Yeast with High Fermentative Activity,Chin. J. Biotechnol., 13, 105 (1997).

[3] Shimizu, H., Araki, K., Shioya, S., Suga, K., Optimal Production of Glutathione by Controlling the Specific Growth Rate of Yeast in Fed-Batch Culture, Biotechnol. Bioeng., 38, 196 (1991).

[4] Sakato, K., Tanaka, H.¸ Advanced Control of Glutathione Fermentation Process, Biotechnol. Bioeng., 40, 904 (1992).

[5] Alfafara, C., Kanda, A., Araki, K., Shimizu, H., Shioya, S., Suga, K., Effect of Amino Acids on Glutathione Production by Saccharomyces cerevisiae, Appl. Microbiol. Biotechnol., 36, 538 (1992).

[6] Alfafara, C., Miura, K., Shimizu, H., Shioya, S., Suga, K., Cysteine Addition Strategy for Maximum Glutathione Production in Fed-Batch Culture of Saccharomyces cerevisiae, Appl. Microbiol. Biotechnol., 37, 141 (1992).

[7] Li, Y., Chen, J., Mao, Y.Y., Lun, S., Koo, Y.M., Effect of Additives and Fed-Batch Culture Strategies on the Production of GSH by Recombinant Escherichia coli, Process. Biochem., 33, 709 (1998).

[8] Liu, C.H., Hwang, Ch.F., Liao, Ch.Ch., Medium Optimization for Glutathione Production by Saccharomyces  cerevisiae,  Process Biochem., 34, 17 (1999).

[9] Wei, G., Li, Y., Du, G.h., Chen, J., Effect of Surfactants on Extracellular Accumulation of Glutathione by Saccharomyces cerevisiae, Process Biochem., 38, 1125 (2003).

[10] Lin, J.P., Tian, J., You, J.F., Jin, Z.H., Xu, Z.N., Cen, P.L., An Effective Strategy for the Co-Production of S-adenosyl-L-Methionine and GSH by Fed-Batch Fermentation, Biochem. Eng. J., 21, 19 (2004).

[11] Liu, H., Lin, J.P., Cen, P.L., Pan, Y.J., Co-Production of S-Adenosyl-L-Methionine and GSH from Spent Brewer’s Yeast Cells, Process Biochem., 39, 1993 (2004).

[12] Wang, Z., Tan, T., Song, J., Effect of Amino Acids Addition and Feedback Control Strategies on the High-Cell-Density Cultivation, Process Biochem., 42, 108 (2007).

[13] Homogenization-Cosmetic,  Pharma  &  Biotech:  http:// www. niroinc. com/ html/ fammap. html., Nov. (2003).

[14] Homogenization Significantly Improves the Quality of Your Product: http://www.bykowskiequipment. com., Nov. (2003).

[15] Alliger, H., Ultrasonic Disruption, Am. Lab., 10, 75 (1975).

[16] Povey, M.J.W., Mason, T.J., Ultrasound in Food Processing, Blackie Academic and Professional, London, UK (1998).

[17] Sauer, T., Robinson, C.W., Glick, B.R., Disruption of Native and Recombinant Escherichia coli in a High Pressure Homogenizer, Biotechnol. Bioeng., 33, 1330 (1989).

[18] Zhang, Z., Blewett, J.M., Thomas, C.R., Modelling the Effect of Osmolality on the Bursting Strength of Yeast Cells, J. Biotechnol., 71, 17 (1999).

[19] John, R.W., Alan, V.Q., Evaluation of the Potential of a Beadmill for the Release of Intracellular Bacterial Enzymes, Enzyme Microb. Technol., 4, 385 (1982).

[20] Lyng, J.G., Allen, P., McKenna, B.M., The Influence of High Intensity Ultrasound Baths on Aspects of Beef Tenderness, J. Muscle Foods, 8, 237 (1997).

[21] Nandakumar, R., Gounot, A.M., Mattiasson, B., Gentle Lysis of Mucous Producing Cold-Adapted Bacteria by Surfactant Treatment Combined with Mechanical Disruption,J. Biotechnol., 83, 211 

Statistics
Article View: 2,046
PDF Download: 1,151
Home | Glossary | News | Aims and Scope | Sitemap
Top Top

web page hit counter

Journal Management System. Designed by sinaweb.