Media Selection for Poly(hydroxybutyrate) Production from Methanol by Methylobacterium Extorquens DSMZ 1340

Document Type: Research Article

Authors

1 Biotechnology Group, Faculty of Engineering, Tarbiat Modares University, P.O.Box 14115-143, Tehran, I.R. IRAN

2 National Nutrition and Food Technology Research Institute, Shahid Beheshti Medical University, P. O. Box 19395-4741, Tehran, I.R. IRAN

Abstract

Plackett-Burman design was used for selection of important media components such as carbon and nitrogen sources and minerals which affect poly(hydroxybutyrate) production and cell growth of Methylobacterium extorquens DSMZ 1340. Among the studied variables, nitrogen and phosphorus sources, MgSO4 and most of the trace elements were found to be significant variables for PHB production from methanol. At best condition (based on PHB concentration), dry cell weight, PHB content and PHB concentration were 3.81 g/L, 21.23 %, and 0.809 g/L, respectively. It was also found that most of the trace elements and phosphorus sources were influential parameters on the growth of microorganism but the kind of nitrogen source was not. The experimental results showed that deficiencies of nitrogen sources (NH4Cl and NH4NO3), phosphorus sources (K2HPO4 and Na2HPO4) and MgSO4 in medium, increased PHB accumulation.  

Keywords

Main Subjects


[1] Khosravi-Darani, K. and Vasheghani Farahani, E., Microorganisms and Systems for Production of Poly(hydroxybutyrate) as an Biodegradable Polymer, Iran. J. Chem. Chem. Eng., 24, p. 1 (2005).

[2] Reddy, C.S.K., Ghai, R., Rashmi, Kalia, V.C., Polyhydroxyalkanoates: An Overview, Bioresource Technol., 87, p. 137 (2003).

[3] Suriyamongkol, P., Weselake, R., Narine, S., Moloney, M. and Shah, S., Biotechnological Approaches for the Production of Polyhydroxy-alkanoates in Microorganisms and Plants, A Review, Biotechnol. Adv.,25, R. 148 (2007).

[4] Bucci, D.Z., Tavares, L.B.B., Sell, I., PHB Packaging for Storage of Food Products, Polymer Testing,
24, p. 564 (2005).

[5] Chen, G.O., Wu, Q., The Application of Polyhydroxy-alkanoates as Tissue Engineering Materials, Biomaterials, 26, p. 6556 (2005).

[6] Van der Walle, G.A., de Koning, G.J., Weusthuis, R.A., Eggink, G., Properties, Modification, and Application of Biopolyesters, Adv. Biochem. Eng. Biot., 71, p. 263 (2001).

[7] Zinn, M., Witholt, B., Egli, T., Occurrence, Synthesis and Medical Application of Bacterial Poly-hydroxyalkanoate, Adv. Drug Deliver. Rev., 53, 5 (2001).

[8] Nikel, P.I., Pettinari, M.J., Mendez, B.S. and Galvagno, M.A., Statistical Optimization of a Culture Medium for Biomass and Poly(3-hydroxybutyrate) Production by a Recombinant Escherichia coli Strain using Agroindustrial Byproducts, Int.Microbiol., 8, p. 243 (2005).

[9] Kim, P., Kim, J.H., Oh, D.K., Improvement in Cell Yield of Methylobacterium sp. Reducing the Inhibition of Medium Components for Poly-b-hydroxybutyrate Production, World J. Microb. Biot., 19, p. 357 (2003).

[10] Ishizaki, A., Tanaka, K., Taga,  N.,  Microbial Production of Poly-3-hydroxybutyrate from CO2, Appl. Microbiol. Biot., 57, p. 6 (2001).

[11] Nath, A. Dixit, M., Bandiya, A., Chavda, S., Desai, A.J., Enhanced PHB Production and Scale Up Studies Using Cheese Whey in Fed Batch Culture of Methylobacterium sp. ZP24, Bioresource Technol., 99, p. 5749 (2008).

[12] Gutierrez, J., Bourque, D., Criado, R., Choi, Y.J., Cintas, L.M., Hernandez, P.E., Miguez, C.B., Heterologous Extracellular Production of Enterocin P from Enterocococcus faecium P13 in the Methyl-otrophic Bacterium Methylobacterium extorquens, FEMS Microbiol Lett., 248, 125 (2005).

[13] Govorukhina,  N.I.  and   Trotsenko,  Y.A.,  Poly-β-hydroxybutyrat Contents of Methylotrophic Bacteria with Different Routes for Methanol Assimilation, Appl. Biochem. Micro., 27, p. 80 (1991).

[14] Bourque, D., Pomerleau  Y.  and  Groleau, D., High-Cell-Density Production of Poly-β-hydroxybutyrate (PHB) from Methanol by Methylobacterium extorquens: Production of High-Molecular-Mass PHB, Appl. Microbiol. Biot., 44, p. 367 (1995).

[15] Choi, J.H.,  Kim, J.H.,  Daneial, M.  and  Lebeault, J.M., Optimization of Growth Medium and Poly-β-hydroxybutyric Acid Production from Methanol in Methylobacterium organophilium, Kor. J. Appl. Microbiol. Bioeng., 17, p. 392 (1989).

[16] Daneil, M., Choi, J.H., Kim, J.H. and Lebeautl, J.M., Effect of Nutrient Deficiency on Accumulation and Relative Molecular Weight of Poly-β-hydroxybutyric Acid by Methylotrophic Bacterium, Pseudomonas 135, Appl. Microbiol. Biot., 37, p. 702 (1992).

[17] Suzuki, T.,  Yamane, T.  and  Shimizu, S.,  Mass Production of Poly-β-hydroxybutyric Acid by Fully Automatic Fed-Batch Culture of Methylotroph, Appl. Microbiol. Biot., 23, p. 322 (1986a).

[18] Yezza, A., Fournier, D., Halasz, A.  and  Hawari, J., Production of Polyhydroxyalkanoates from Methanol by a New Methylotrophic Bacterium Methyl-obacterium sp. GW2, Appl. Microbiol. Biot., 73, p. 211 (2006).

[19] Plackett, R.L., Burman, J.P., The Design of Optimum Multifactorial Experiments, Biometrika,33, p. 305 (1946).

[20] Levin, L., Forchiassin, F. and Viale, A., Ligninolytic Enzyme Production and Dye Decolorization by Trametes Trogii: Application of the Plackett-Burman Experimental Design to Evaluate Nutritional Requirements, ProcessBiochem.,40, p. 1381 (2005).

[21] Naveena, B.J., Altaf, Md., Bhadriah, K. and Reddy, G., Selection of Medium Component by Plackett-Burman Design for Production of L(+)lactic acid by Lactobacilus amylophilus GV-6 in SSF Using Wheat Bran, Bioresource Technol., 96, p. 485 (2005).

[22] Gohel, V., Chaudhary, T., Vyas, P. and Chhatpar, H.S., Statistical Screening of Medium Component for the Production of Chitinase by the Marine Isolate Pantoea dispersa, Biochem. Eng. J., 28, p. 50 (2006).

[23] Chauhan, K., Trivedi, U. and Patel, K.C., Statistical Screening of Medium Components by Plackett-Burman Design for Lactic Acid Production by Lactobacillus sp. KCP01 using Date Juice, Bioresource Technol., 98, p. 98 (2007).

[24] Khosravi-Darani, K., Vasheghani-Farahani, E. and Shojaosadati, S.A., Application of the Plackett-Burman Design for the Optimization of Poly(β-hydroxybutyrate) Production by Ralstonia eutropha, Iran. J. Biotechnol., 1, p. 155 (2003).

[25] Khosravi-Darani, K., Vasheghani-Farahani, E. and Shojaosadati, S.A., Application of the Taguchi Design for Production of poly(β-hydroxybutyrate) by Ralstonia eutropha, Iran. J. Chem. Chem. Eng., 23, p. 131 (2004).

[26] Braunegg,  G.,  Sonnleitner,  B.  and  Lafferty  R.M., A Rapid Gas Chromatographic Method for the Determination of Poly-β-hydroxybutyric Acid in Microbial Biomass, Eur. J. Appl. Microbiol. Biot.,
6, p. 29 (1987).

[27] Monaghan, R. L. and Koupal, L.R., Use of the Plackett Burman Technique in a Discovery Program for New Natural Products, In: “Topics InIndustrial Microbiology”, Editors: A. Demain, G. Somkuti, J. Hunter Cevera and H. W. Rossmoore, pp. 25-32 (1989).