Comparative Study on the Ni2+ Biosorption Capacity and Properties of Living and Dead Pseudomonas putida Cells

Document Type : Research Article

Authors

State Key Laboratory of Pollution Control and Resource Reuse, School of Environmental Science and Engineering, Tongji University, Shanghai 200092, CHINA

Abstract

Microbial cells have been successfully used as biosorbents to remove heavy metals from wastewater. In some cases, dead cells appear to offer more advantages than living cells in the removal and recovery of heavy metal ions from industrial wastewater. Maintaining higher biosorption capability and understanding the biosorption properties of dead cells are the keys to heavy metal removal and recovery from wastewater using dead cells as biosorbents. The present experiment showed that the dead Pseudomonas putida 5-x cells killed by dilute HCl had a higher Ni2+ biosorption capacity due to the retention of a complete cell structure during the acid treatment process. The biosorption process of the dead cells was faster than that of the living cells. Metabolic-independent physical adsorption played a major role in the Ni2+ sorption by the dead cells.The pH obviously affected the biosorption capacity of the dead cells, because of the variation of the hydrogen ion concentration and cell surface property, as well as occurrence of micro-precipitation along with the change of solution pH. Considering both biosorption capacity and desorption efficiency, pH 6.5-7.0 is a suitable condition for Ni2+ biosorption by dead P. putida 5-x cells killed with dilute HCl.

Keywords

Main Subjects

References

[1] James A.M., The Electrical Properties and Topochemistry of Bacterial cells, Advances in Colloid and Interface Science, 15, p. 171 (1982).
[2] Beveridge T.J., Forsberg C.W., Doyle R.J., Major sites of Metal Binding in B. lichenformis walls, Journal of Bacteriolog, 150, p. 1438 (1982).
[3] Doyle R.J., Matthews T.H., Streips U.N., Chemical Basis for Selectivity of Metal ions by the Bacillus  Subtilis Cell Wall, Journal of Bacteriolog, 143, p. 471 (1980).
[4] Kao W.C., Huang C.C., Chang J.S., Biosorption of Nickel, Chromium and Zinc by MerP-Expressing Recombinant Escherichia coli, Journal of hazardous materials, 158, p. 100 (2008).
[5] Mata Y.N., Blazquez M.L., Ballester A., Gonzalez F., Munoz J.A.,Characterization of the Biosorption of Cadmium, Lead and Copper with the Brown Alga Fucus Vesiculosus, Journal of hazardous materials 158, p. 316 ( 2008).
[6] Warren L.A., Ferris F.G., Continuum Between Sorption and Precipitation of Fe (III) on Microbial Surfaces, Environmental Science Technology, 32, p. 2331 (1998).
[7] Yee N., Fein J., Cd Adsorption Onto Bacterial Surface: A Universal Adsorption Edge, Geochim. Cosmochim. Acta, 65, p. 2037 (2001).
[8] Yetis U.E., Oezcengiz G., Dilek F.B., Ergen N., Erbay A., Doelek A., Heavy Metal Biosorption by White-Rot Fungi, Water Science Technolog, 38, p. 323 (1998).
[9] Gupta V.K., Rastogi A., Biosorption of Lead(II) from Aqueous Solutions by Non-Living Algal Biomass Oedogonium sp and Nostoc sp - A Comparative Study, Colloids and surface B-biointerfaces,64, p. 170 (2008).
[10] Gadd G.M., White C., Removal of Thorium from Simulated Acid Process Stream by Fungal Biomass: Potential for Thorium Desorption and Reuse of Biomass and Desorbent, J. Chem. Technol. Biotechnol., 55, p. 39 (1992).
[11] Matheickal J.T., Yu Q., Biosorption of Lead(II) and Copper(II) from Aqueous Solutions by Pre-Treated Biomass of Australian Marine Algae, Bioresource Technology, 69, p. 223 (1999).
[12] Schiewer S., Volesky B., Biosorption Process for Heavy Metal Removal, "In: Environmental Microbe-Metal Interactions",Derek R. L., (Ed.), ASM Press Inc., Washington DC, pp. 329-362(2000).
[13] Wong P.K., So C.M., Cu2+ Accumulation by a Strain of Pseudomonas putida, Microbiology, 73, p. 113 (1993).
[14] Sze K.F., Lu Y.J., Wong P.K., Removal and Recovery of Copper ion (Cu2+) from Electroplating Effluent by a Bioreactor Containing Magnetite-Immobilized Cells of Pseudomonas putida, Resources, Conservation and Recycling, 18, p. 175 (1996).
[15] Wang L., Li F.T., Zhou Q., Contribution of Cell Surface Components to Cu2+ Adsorption by P. putida 5-x, Applied Biochemistry and Biotechnology, 128, p. 33 (2006).
[16] Environmental Management Division. Guidebook on the Treatment of Wastewater for the Hong KongElectroplating Industry. Hong Kong Productivity Council: Hong Kong, (1986).
[17] Taylor M.C., Demayo A., Reeder S.W., Inorganic Chemical Substances-Nickle. In Guidelines for Surface Water Quality; Environment Canada: Ottaw
[18] Codina J.C., Perez-Garcia A., Vicente A.D., Detection of Heavy Metal Toxicity and Genotoxicity in Wastewater by Microbial Assay, Water Science & Technology, 30, p. 145 (1994).
[19] Wang L., Chua H., Sin S.N., Zhou Q., Ren D.M., Li Z.L., A Combined Bioprocess for Integrated Removal of Copper and Organic Pollutant from Copper-zontaining Municipal Wastewater, J. Environ. Sci. Heal. Part (A), 39, p. 223 (2004).
[20] Hu Z.C., Norman J.M., Faison B.D., ReeresM.E., Biosorption of Uranium by P. Aeruginosa Strain CSO: Characterization and Comparison Studies, Biotech. Bioeng., 51, p. 237 (1996).
[21] Townsley C.C., Ross L.S., Atkins A.S., Copper Removal from a Simulated Leach Effluent Using the Filamentous Fungus Trichoderma viride, "In: Immobilisation of Ions by Bio-sorption", Eccles H., Hunt, S. S., (Eds.), Ellis Horwood,Chichester, pp.159-170 (1988).
[22] Komori K., Rivas A., Toda K., Ohtake H., Biological Removal of Toxic Chromium Using an Enterobacter cloacae Strain that Reduces Chromate Under Anaerobic Conditions, Biotechnology and Bioengineering, 35, p. 951 (1990).
[23] Ishibashi Y., Cervantes C., Silver S., Chromium reduction by Pseudomonas putida, Applied and Environmental Microbiology, 56, p. 2268 (1990).
[24] Kuyucak N., Volesky B., Biosorbents for Recovery of Metals from Industrial Solutions, Biotechnol. Lett., 10, p. 137 (1988).

Files

Share

How to cite

Statistics