Adsorption of Bovine Serum Albumin onto Hydroxylapatite: Theoretical Modeling and Measurements

Document Type: Research Article

Authors

Faculty of Chemical Engineering, Amirkabir University of Technology, Tehran, I.R. IRAN

Abstract

Adsorption of Bovine Serum Albumin (BSA) onto calcium Hydroxyl Apatite (HA), Ca10PO4(OH)2, has been studied by Ultra Violet (UV) spectroscopy. The adsorption isotherms of BSA onto the HA surface at 291.7 K and 303.2 K were satisfactorily presented by Langmuir equation and the evaluated parameters are reported. The specific surface area of HA has been measured by the BET method. The obtained value for the used HA sample was 63 m2/g. The effect of pH on BSA adsorption onto the HA in presence of salts KCl and NaF and phosphate ion has been investigated. In the fixed concentration of BSA (1 mg/mL) it is shown that the pH decreasing causes to increase of the adsorption of BSA onto HA. Considering the role of effective factors in BSA adsorption onto HA such as the size and charge of the BSA and the surface energy of HA showed that the electrostatic repulsion forces between HA and BSA cause a decrease in BSA adsorption onto HA. Also the obtained results indicated that the surface energy of HA predominates the effect of size and charge of BSA.

Keywords

Main Subjects


[1] Hench L.L., Stability of Ceramics in the Physiological Environment. In: D.F. Williams, Editor, "Fundamental Aspects of Biocompatibility", CRC Press, Boca Raton, FL (1981).

[2] Gorbunoff M.J., The Interaction of Proteins with Hydroxylapatite. I. Role of Protein Charge and Structure, Anal. Biochem., 136, p. 425 (1984a).

[3] Gorbunoff M.J., Timasheff S.N., The Interaction of Proteins with Hydroxylapatite. III. Mechanism, Anal. Biochem., 136, p. 440 (1984).

[6] Gorbunoff M.J., The Interaction of Proteins with Hydroxylapatite. II. Role of Acidic and Basic Groups, Anal. Biochem., 136, p. 433 (1984b).

[7] Graham E.E., Pucciani A./ Pinto N.G., A Comparative Study of Models to Predict Protein Adsorption, Biotechnology Progress, 3, p. 141 (1987).

[8] Shi D., Jiang G., Wen X., In Vitro Bioactive Behavior of Hydroxylapatite-Coated Porous Al2O3, J. of Biomedical Material Research, Applied Biomaterials, 53, p. 475 (2000). 

[9] Kandori K., Fudo A., Ishikawa T., Study on the Particle Texture Dependence of Protein Adsorption by Using Synthetic Micrometer-Sized Calcium Hydroxylapatite Particles, Colloids and Surfaces B: Biointerfaces, 24, p. 145 (2002).

[10] Sharpe J.R., Sammons R.L., Marquis P.M., Effect of pH on Protein Adsorption to Hydroxylapatite and Tricalcium Phosphate Ceramics, Biomaterials, 18, p. 471 (1997).

[11] Norde W., Adsorption of Proteins from Solution at the Solid-Liquid Interface, Adv Colloid Interface Sci., 25, p. 267 (1986).

[12] Andrade J.D., "Surface and Interfacial Aspects of Biomedical Polymers, Surface Chemistry and Physics", Plenum Press, New York, (1985).

[13] Brash J.L., Horbett T.A., "Proteins at Interface", ACS Symposium Series, 602, Washington DC, (1995).

[14] Andrade J.D., Hlady V., Peng L., Yingery K., "Interfacial Phenomena and Bioproducts",Dekker,New York, (1996).

[15] Jaffe W.L., Scott D.F., Total Hip Arthroplasty with Hydroxylapatite-Coated Prostheses, J. Bone Joint Surg., 78A, p. 1918 (1996).

[16] LeGeros R.Z., "Calcium Phosphates in Oral Biology and Medicine", Chap. 6, H. M. Myers, ed., Karger, Basel (1991).

[17] Chow L.C., Sun L., Hockey B., Properties of Nanostructured Hydroxylapatite Prepared by a Spray Drying Technique, Res. Natl. Stand. Technol., 109, p. 543 (2004).

[18] Fragues C., Bailly M., Grevillot G., Adsorption of BSA and Hemoglobin on Hydroxylapatite Support: Equilibria and Multicomponent Dynamic Adsorption, Adsorption, 4, p. 5 (1998).

[19] Kawasaki T., Niikura M., Takahashi S., Kobayashi, W., Strontium-Phosphate Hydroxylapatite High-Performance Liquid Chromatography, Biochem. Int., 15, p. 1137 (1987).

[20] Kawasaki T., Kobayashi W., Ikeda K., Takahashi S., Monma H., High-Performance Liquid Chromatography using Spherical Aggregates of Hydroxylapatite Micro-Crystals as Adsorbent, Eur. J. Biochem., 157, p. 291 (1986).

[21] Kazuhiko K., Yumi U., Tatsuo I., Effects of Acetonitrile on Adsorption Behavior of Bovine Serum Albumin onto Synthetic Calcium Hydroxylapatite Particles, J. of Colloid and Interface Science, 252, p. 269 (2002).

[22] Creighton T.H., "Proteins",Freeman,New York, (1993).

[23] Franks F., "Characterization of Proteins", Humana, New Jersey, 1988.

[24] Mysels K.J., "Introduction to Colloid Chemistry",Krieger,New York, (1978).

[25] Tanford C., "Physical Chemistry of Macromolecules", Wiley, New York, (1961).

[26] Luo Q., Andrade J.D., Cooperative Adsorption of Proteins onto Hydroxylapatite, J. of Colloid and Interface Science, 200, p. 104 (1998).

[27] Antonelli M.L., Capalbi A., Gente,G., Palacios, A.C., Sallustio S., and MesaC.L., Thermodynamic Properties of the Bovine Serum Albumin-Sodium Taurodeoxycholate System, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 246, p. 127 (2004).

[28] Lu R.C., Cao A.N., Lai L.H., Xiao J.X., Interaction Between β-Lactoglobulin and Perfluorooctanoate Surfactants: Effect of Surfactant Counterion, Colloids and Surfaces A: Physicochemical and Engineering Aspects, 292, p. 279 (2007).

[29] Pekel N., Salih B., Güven O., Metal ion Promoted Hydrogels for Bovine Serum Albumin Adsorption: Cu(II) and Co(II) Chelated Poly[(N-vinylimidazole)-Maleic Acid], Colloids and Surfaces B: Biointerfaces, 42, p. 89 (2005).

[30] Brunauer S., Emmett P.H., Teller E., Adsorption of Gases in Multimolecular Layers, J. Am. Chem. Soc., 60, 309 (1938).

[31] Verwilghen, C., Rio, S., Nzihou, A., Gauthier, D., Flamant, G. and Sharrock, P.J., Preparation of High Specific Surface Area Hydroxylapatite for Environmental Applications, J. Mater Sci., 42, p. 6062 (2007).

[32] Kopac T., Bozgeyik K., Yener J., Effect of pH and Temperature on the Adsorption of Bovine Serum Albumin onto Titanium Dioxide, Colloids and Surfaces A: Physicochemical Engineering Aspects, 322, p. 19 (2008).

[33] Beigi M. et al., Batch Equilibrium and Kinetics Studies of Cd (II) Ion Removal from Aqueous Solution Using Porous Chitosan Hydrogel Beads, Iran. J. Chem. Chem. Eng., 28, p. 3 (2009).

[34] Freundlich HMF., Over the Adsorption in Solution, J. Phys. Chem., 57 (1906).

[35] Kandori K., ShimizuT., Yasukawa A., Kandori K., Adsorption of Bovine Serum Albumin onto Synthetic Calcium Hydroxylapatite: Influence of Particle Texture, Colloids and Surfaces B: Biointerfaces, 5, p. 81 (1995).

[36] Kawasaki T., Ikeda K., Takahashi S., Kuboki Y., Further Study of Hydroxylapatite High-Performance Liquid Chromatography using Both Proteins and Nucleic Acids, and a New Technique to Increase Chromatographic Efficiency, Eur. J. Biochem., 155, p. 249 (1986).

[37] Yin G., Liu Z., Zhan J., Ding F., Yuan N., Impacts of the Surface Charge Property on Protein Adsorption on Hydroxylapatite, Chemical Engineering Journal, 87, p. 181 (2002).