A Microcalorimetry Study of the Binding of Nickel Ion by Human Growth Hormone

Document Type : Research Article

Authors

Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, I.R. IRAN

Abstract

A binding study of nickel ions by a new recombinant human Growth Hormone (hGH), produced as an injected drug, has been done at 27˚C in NaCl solution (50 mM) using an isothermal titration calorimetry. There is a set of three identical and non-interacting binding sites for nickel ions. The intrinsic dissociation equilibrium constant and the molar enthalpy of binding are 40 μM and -16.5 kJ/mol, respectively. Thermodynamic parameters of nickel ion binding are compared to the other metal ions. The molar entropy of binding is 29.3 J K-1 mol-1 for Ni2+, less than Cu2+ and more than other metal ions, means that the disorder of the protein structure due to the binding of nickel ions is more than to the other ion metals, except Cu2+. It is expected that nickel ions can prevent from the aggregation of the protein.

Keywords

Main Subjects

References

[1] Hindmarsh P.C., Brook C.G., Effect of Growth Hormone on Short NormalChildren, Br. Med. J. (Clin. Res. Ed.), 295, p. 573 (1987).
[2] Raiti S., Advance in Human Growth Hormone Research, Department of Health, Education and Welfare (DHEW):WashingtonDC; Publication No. (NIH) 74612, 409-433 (1974).
[3] Wallis M., Howell S.L.,TaylorK.W., "In the Biochemistry of the Polypeptide Hormone", John Wiley & Sons:New York, pp 185-222 (1985)
[4] de Voc A.M., Ultsch M., Kossiakoff A.A., Human Growth Hormone and Extracellular Domain of its Receptor: Crystal Structure of the Complex, Science, 255, p. 306 (1992).
[5] Kasimova M.R., Kristensen S.M., Howe P.W.A., Christensen T., Matthiesen F., Petersen J., Sّrensen H.H., Led J.J., NMR Studies of the Backbone Flexibility and Structure of Human Growth Hormone: a Comparison of High and Low pH Conformations, J. Mol. Biol., 318, p. 679 (2002).
[6] Filikov A.V., Hayes R.J., Luo P., Stark D.M., Chan C., Kundu A., Dahiyat B.I., Computational Stabilization of Human Growth Hormone, Protein Sci., 11, p. 1452 (2002).
[7] Youngman K.M., Spencer D.B., Brems D.N., DeFelippis M.R., Kinetic Analysis of the Folding of Human Growth Hormone. Influence of Disulfide Bonds, J. Biol. Chem., 270, p. 19816 (1995).
[8] Brems D.N., Brown P.L., Becker G.W., Equilibrium Denaturation of Human Growth Hormone and Its Cysteine-Modified Forms, J. Biol. Chem., 265, p. 5504 (1990).
[9] Cunningham B.C., Mulkerrin M.G., Wells J.A., Dimerization of Human Growth Hormone by Zinc, Science, 253, p. 545 (1991).
[10] Dienys G., Sereikaite J., Luksa V., Jarutiene O., Mistiniene E., Bumelis V.A., Dimerization of Human Growth Hormone in the Presence of Metal Ions, Bioconjugate Chem., 11, p. 646 (2000).
[11] Yang T.H., Cleland J.L., Lam X., Meyer J.D., Jones L.S., Randolph T.W., Manning M.C., Carpenter J.F., Effect of Zinc Binding and Precipitation on Structures of Recombinant Human Growth Hormone and Nerve Growth Factor, J. Pharm. Sci. , 89, p. 1480 (2000).
[12] Hovorka S.W., Williams T.D., Schoneich C., Characterization of the Metal-Binding site of Bovine Growth Hormone Through Site-Specific Metal-Catalyzed Oxidation and High-Performance Liquid Chromatography-Tandem Mass Spectrometry, Anal. Biochem., 300, p. 206 (2002).
[13] Saboury A.A., Atri M.S., Sanati M.H., Sadeghi M., Application of a Simple Calorimetric Data Analysis on the Binding Study of Calcium Ions by Human Growth Hormone, J. Therm. Anal. Cal., 83, p. 175 (2006).
[14] Saboury A.A., Atri M.S., Sanati M.H., Moosavi-Movahedi A.A., Haghbeen K., Effects of Calcium Binding on the Structure and Stability of Human Growth Hormone, Int. J. Biolog. Macromol., 36, p. 305 (2005).
[15] Saboury A.A., Atri M.S., Sanati M.H., Moosavi-Movahedi A.A., Hakimelahi G.H., Sadeghi M., A Thermodynamic Study on the Interaction between Magnesium Ion and Human Growth Hormone, Biopolymers, 81, p. 120 (2006).
[16] Atri M.S., Saboury A.A., Rezaei-Tavirani M., Sanati M.H., Moosavi-Movahedi A.A., Sadeghi M., Mansuri-Torshizi H., Khodabandeh N., Binding Properties and Conformational Change of Human Growth Hormone upon Interaction with Fe+3", Thermochim. Acta, 438, p. 178 (2005).
[17] Saboury A.A., Kordbacheh M., Sanati M.H., Mizani F., Shamsipur M., Yakhchali M.B., Moosavi-Movahedi A.A., Thermodynamics of Binding Copper Ion by Human Growth Hormone, Asian J. Chem., 17, 2773 (2005)
[18] Saboury A.A., Ghourchaei H., Sanati M.H., Atri M.S., Rezaei-Tawirani M., Hakimelahi G.H., Binding Properties and Structural Changes of Human Growth Hormone upon Interaction with Cobalt Ion, J. Therm. Anal. Cal., 89, p. 921 (2007).
[19] Saboury A.A., Atri M.S., Metal Ions Binding Study on Human Growth Hormone by Isothermal Titration CalorimetryMetal Ions binding Study on Human Growth Hormone by Isothermal Titration Calorimetry, J. Phys. Theo. Chem., 2, p. 169 (2006).
[20] LancasterJ.R., "The Bioinorganic Chemistry of Nickel", VCH Publishers,Weinheim,Germany(1988).
[21] Thauer R.K., Diekert G., Schonheit P., Biological Role of Nickel, Trends Biochem. Sci., 5, p. 304 (1980).
[22] Hausinger R.P., Nickel Utilization by Microorganisms, Microbiol. Rev., 51,p. 22 (1987).
[23] Saboury A.A., Hosseini-Kishani F., Rezaei-Tawirani M., Ranjbar B., Thermodynamic Studies on the Interaction of Nickel with Human Serum Albumin, Prog. Biochem. Biophys., 30, p. 732 (2003).
[24] Bewley T.A., BrovettoCruz J., Li C.H., Human Pituitary Growth Hormone, Physicochemical Investigations of the Native and Reduced-Alkylated Protein, Biochemistry, 8, p. 4701 (1969).
[25] Saboury A.A., Divsalar A., Ataie G., Moosavi-Movahedi A.A., Housaindokht M.R., Hakimelahi G.H., A Product Inhibition Study on Adenosine Deaminase by Spectroscopy and Calorimetry, J. Biochem. Mol. Biol., 35, p. 302 (2002).
[26] Sarri-Sarraf N., Saboury A.A., Moosavi-Movahedi A.A., Product Inhibition Study on Carbonic Anhydrase Using Spectroscopy and Calorimetry, J. Enz. Inhib. Med. Chem., 17, p. 203 (2002).
[27] Saboury A.A., New Methods for Data Analysis of Isothermal Titration Calorimetry, J. Therm. Anal. Cal., 72, p. 93 (2003).
[28] Saboury A.A., A Review on the Ligand Binding Studies by Isothermal Titration Calorimetry, J. Iran. Chem. Soc., 3, p. 1 (2006).

Files

Share

How to cite

Statistics