Decolorization of Cibacron Black w-55 under Alkaline Conditions by New Strain of Halomonas sp. Isolated from Textile Effluent

Document Type: Research Note

Authors

1 Biotech. Lab., Department of Soil Science, University College of Agriculture and Natural Resources, University of Tehran, Tehran, I.R. IRAN

2 Extermophiles Lab., Department of Microbiology, School of Biology, University College of Science, University of Tehran, Tehran, I.R. IRAN

3 Department of Parasitology, Pasteur Institute of Iran, Tehran, I.R. IRAN

Abstract

Among the 30 strains of moderately halophilic bacteria isolated from the salty effluents of textile industries around Qom city in central Iran, a Gram-negative rod shape bacterium designated as strain IP8 showed a remarkable ability in decolorizing of azo dyes over wide ranges of pH (7-11) and temperature (25-45 ºC), in presence of NaCl and Na2SO4 (0.5-1.5 M) under anaerobic and aerobic conditions. Phenotypic characterization and phylogenetic analysis based on 16S rDNA sequence comparisons indicated that this strain was a member of the genus Halomonas with the greatest similarity to Halomonas axialensis. UV-Vis analysis before and after decolorization and the colorless bacterial biomass after treatment suggested that decolorization was due to biodegradation. HPLC analysis of dye treatment confirmed that the principal biodegradation was occurred after 48h of incubation in aeration culture. The effect of metal salts on decolorization showed that AgNO3 and NaAsO4 were of higher and lower effect on decolorization, respectively.

Keywords

Main Subjects


[1] Chen K.C., Wu J.Y., Liou D.J., Hwang S.J., Decolorization of Textile Dyes by Newly Isolated Bacterial Strains, J. Biotechnol., 101, p. 57 (2003).
[2] Rahmani A.R., Zarrabi M., Samarghandi M,R., Afkhami A., Gaffari H.R., Degradation of Azo Dye Reactive Black 5 and Acid Orange 7 by Fenton-Like Mechanism, Iran. J. Chem. Eng, 7, p. 87 (2010).
[3] Sharma D.K., Saini H.S., Singh M., Chimni S.S., Chadha B.S., Biodegradation of Acid Blue-15, a Textile Dye, by an Up-Flow Immobilized Cell Bioreactor, J. Ind. Microbiol. Biotechnol, 31, p. 109 (2004).
[4] Forgacs E., Cserhati T., Oros G., Removal of Synthetic Dyes from Wastewaters: a Review., Environ. Int. J., 30, p. 953 (2004).
[5] Asad S., Amoozegar M.A., Pourbabaee A.A.., Sarbolouki M.N., Dastgheib S.M.M., Decolorization of Textile Azo Dyes by Newly Isolated Halophilic and Halotolerant Bacteria, Bioresour. Technol, 98, p. 2082 (2007).
[6] Zimmermann T., Kulla H.G., Leisinger T., Properties of Purified OrangeII Azo Reductase, the Enzyme Initiating Azo Dye Degradation by Pseudomonas KF46. J. Biochem, 129, p. 197 (1982).
[7] Pourbabaee A.A., Malekzadeh F., Sarbolouki M.N., Mohajeri, A., Decolorization of Methyl Orange(as a Model Azo Dye) by the Newly Discovered Bacillus SP., Iran. J. Chem. Chem. Eng., 24(3), p. 41 (2005).
[8] Karimi A., Vahabzadeh F., Mohseni M., Decolorization of Maxilon-Red by Kissiris Immobilized Phanerochaete Chrysosporium in a Trickle- Bed Bioreactor-Involvement of Ligninolytic Enzymes, Iran. J. Chem. Chem. Eng., 28(2), p. 1 (2009).
[9] Vahabzadeh F, Mogharei A, Mehranian M., Decolorization of Molasses Wastewater from an Alcoholic Fermentation Processes with Phanerochate Chrisosporium- Involvement of Ligninase, Iran. J. Chem. Chem. Eng., 21(2), p. 119 (2002).
[10] Gold M.H., Alic M., Molecolar Biology of Lignin-Degrading Basidomycete Phanerochate Chrysosporium. Microbiol. Rev., 57, p. 605 (1993).
[11] Swamy J., Ramsay J.A., The Evaluation of white rot Fungi in the Decolorization of Textile Dyes, Enzyme Microb. Technol, 24, p. 130 (1999).
[12] Martins M.A.M., Cardos M.H., Queriza M.J., Ramalho M.T., Campos A.M.O., Biodegradation of Azo Dyes by Yeast Cardida zeylanoides in Batch Aerated Cultures, Chemosphere, 38, p. 2455 (1999).
[13] Zhou W., Zimmermann W., Decolorization of Industrial Effluents Contaming Reactive Dyes by Actinomycetes, FEMS Microbiol. Lett, 107, p.157 (1993).
[14] Dilek F.B., Taplamacioglu H.M., Tarlan E., Colour and AOX Removal from Pulping Effluents by Algae, Appl. Microbiol. Biotechnol., 52, p. 585 (1999).
[15] Carliell C.M., Barclay S.J., Shaw C., Wheatley A.D., Buckley C.A., The effect of Salts used in Textile Dyeing on Microbial Decolorisation of a Reactive Dye. Environ. Technol., 19, p. 1133 (1998).
[16] Ventosa A., Quesada E., Rodriguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A., Numerical Taxonomy of Moderately Halophilic Gram-Negative Rods, J. Gen. Microbiol, 128, p. 1959 (1982).  
[17] Quesada E., Ventosa A., Ruiz-Berraquero F., Ramos-Cormenzana A., Deleya halophila, a New Species of Moderately Halophilic Bacteria, Int. J. Syst. Bacteriol, 34, p. 287 (1984).
[18] Aksu Z., Reactive Dye Bioaccumulation by Saccharomyces cerevisiae, Process Biochem., 38, p. 1437 (2003).
[19] Sandrin T.R., Maier R.M., Impact of Metals on the Biodegradation of Organic Pollutants, Env. Health pres, 111, p. 1093 (2003).
[20] Amor L., Kennes C., Veiga M.C., Kinetics of Inhibition in the Biodegradation of Monoaromatic Hydrocarbons in Presence of Heavy Metals, Bioresorce technol, 78, p.181 (2001).
[21] Dong X., Zhou J., Liu,Y., Peptone-induced biodecolorization of Reactive Brilliant Blue (KN-R) by Rhodocyclus Gelatinosus XL-l, Process Biochem, 39, p. 89 (2003)
[22] Akintokun Ola, Akpan A.K., Omomowe I.o.,Areo V.O., Aerobic Decolorization of Two Reactive Azo Dyes under Varing Carbon and Nitrogen Sources by Bacillus Cereus, Afr. J. Biotechnol. 9, p. 672 (2010).
[23] Katia M.G Machale., Luciana C.A. Compit, Rubio Morais., Luiz H. Rosa., Merica H, Santos. Biodegradation of Reactive Textile Dyes by Basidiomycetous Fungi from Brazilian Ecosystems, Braz. J. Microbiol, 37, p. 481 (2006).