Poultry Institute, Chinese Academy of Agricultural Science, Yangzhou 225003, CHINA
A method to quick detect concentration of lecithin in eggs, namely microwave digestion spectrophotometry, was established in this research. The homogenate of eggs was treated with absolute ethanol to eliminate phosphate protein in eggs which could possibly affect concentration of lecithin examined. A sample then received a new way of pre-treatment, called microwave digestion, before UV-Vis spectrometry was applied to examine the concentration of phosphate at 400nm. The linear equation was A = 0.08628X (μg), the corresponding coefficient of correlation was 0.9998,the detection limit of phosphorous was 0.2mg (n=11). The content of lecithin in eggs was then obtained. According to the result, the recovery of 90% was secured; therefore the conclusion of high degree of accuracy was reached.
 Li Y., "Study of Analyses and Refine Method of Lecithin in Soybean", Zhejiang University, Hangzhou, (2004).
 Kuligowski J., Quintás G., Garrigues S., M. de la Guardia, Determination of Lecithin and Soybean Oil in Dietary Supplements Using Partial Least Squares-Fourier Transform Infrared Spectroscopy, Talanta, 77, p. 229 (2008).
 Campanella L., Magrì A.L., Sorbo A., Tomassetti M., Determination of Lecithin Drug Specialties and Diet Integrators; by Means of First or Second Derivative Enzymatic-Spectrophotometric Analysis, J. Pharm. Biomed. Anal.,29, p. 1135 (2002).
 Campanella L., Pacifici F., Sammartino M.P., Tomassetti M., Analysis of Lecithin in Pharmaceutical Products and Diet Integrators Using a New Biosensor Operating Directly in Non Aqueous Solvent, J. Pharm. Biomed. Anal., 18(4-5), p. 597 (1998).
 Heshmati A., Khodadadi I., Reduction of Cholesterol in Beef Suet Using Lecithin, J. Food Composition and Analysis, 22(7-8), p. 684 (2009).
 Pardeike J., Hommoss A., Müller R.H., Lipid Nanoparticles (SLN, NLC) in Cosmetic and Pharmaceutical Dermal Products, Int. J. Pharm., 366(1-2), p. 170 (2009).
 Zhou H., Xia H.T., Lian G.X., Jing P., Determination of Phosphatidylcholine by High Performanceliquid Chromatography in the Soybean Mixed Phospholipid, J. Qiqihar University, 18(4), p. 33 (2002).
 Gong Y., Wang Q., Yang Y., Ding M.Y., Determination of Egg-Yolk Phosphatidylcholine by Normal-phase High Performance Liquid Chromatography with Evaporative Light Scattering Detection, Chinese Journal of Chromatography, 24(4), p. 373 (2006).
 Noula C., Bonzom P., Brown A., Gibbons W.A., Martin J., Nicolaou A., 1H-NMR Lipid Profiles of Human Blood Platelets; Links with Coronary Artery Disease, Biochim. Biophys. Acta., 1487(1), p. 15 (2000).
 Chen W.T., Zhang D.Q., Zhang B.L., Zhou H.J., Determination of Lecithin in Functional Food by UV Spectrophotometry, J. The Chinese Cereals and Oils Association, 21(3), p. 189 (2006).
 Guan M., Li Y., Zhao S.X., Determination of Phosphatidylcholine in Lecithin Capsule by UV, China Brewing, (23), p. 95 (2008).
 Ning Z.X., "Food Composition Analysis Manual[M]", China Light Industry Press, Beijjing, p.171 (1997).