Studies on the Binding of DNA with the Inclusion of Brilliant Green inside the Cavity of γ-Cyclodextrin

Document Type : Research Article

Authors

Department of Chemistry, College of Material Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan 621010, CHINA

Abstract

The interaction of brilliant green with herring sperm DNA was investigated in detail by spectrometric methods in γ-cyclodextrin systems. On the condition of physiological pH, brilliant green prefers to form the 1:1 inclusion complex with γ-cyclodextrin.All the evidences indicated that the binding modes between γ-cyclodextrin-brilliant green and DNA were grooving binding and partial non-classical intercalative binding. The binding ratio of the inclusion complex with DNA is 6:1. The calculated thermodynamic parameters suggested that the binding of the inclusion complex to DNA was driven mainly by entropy.

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References

[1] Wang Y., Zhou A.H., Spectroscopic Studies on the Binding of Methylene Blue with DNA by Means of Cyclodextrin Supramolecular Systems, J. Photochem. Photobiol., A. 190, p. 121 (2007).
[2] Banville D.L., Marzilli L.G., Strickland J.A., Wilson W.D., Comparison of the Effects of Cationic Porphyrins on DNA Properties: Influence of GC Content of Native and Synthetic Polymers. Biopolymers, 25, p. 1837 (1986).
[3] Strickland J.A., Banville D.L., Wilson W.D., Marzilli L.G., Metalloporphyrin Effects on Properties of DNA, Polymers Inorg. Chem., 26: p. 3398 (1978).
[4] Mukundan N.E., Petho G., Dixon D.W., Kim M.S., Marzilli L.G., Interactions of an Electron-Rich Tetracationic Tentacle Porphyrin with Calf Thymus DNA, Inorg. Chem., 33: p. 4676 (1994).
[5] Pasternack R.F., Gibbs E.J., Villafranca J.J., Interactions of Porphyrins with Nucleic Acids, Biochemistry, 22, p. 2406 (1983).
[6] Carvlin M.J., Datta-Gupta N., Fiel R.J., Circular Dichroism Spectroscopy of a Cationic Porphyrin Bound to DNA, Biochem. Biophys. Res. Commun, 108, p. 66 (1982).
[7] Nandi B.K., Goswami A., Purkait M.K., Adsorption Characteristics of Brilliant Green Dye on Kaolin, J. Hazard. Mater., 161, p. 387 (2009).
[8] Zhao G.C., Zhu J.J., Chen H.Y., Wang X.M., Lu Z.H., Spectroscopic and Spectroelectrochemical Studies of Interaction of Nile Blue with DNA, Chin. J. Chem., 20, p. 57 (2002).
[9] An W.T., Guo X.L., Shuang S.M., Dong C., Effect of Microscopic Environment on the Self-Stacking Binding of Porphyrin to DNA, J. Photochem. Photobiol. A., 173, p. 36 (2005).
[10] Zhao G.C., Zhu J.J., Zhang J.J., Chen H.Y., Voltammetric Studies of the Interaction of Methylene Blue with DNA by Means of β-Cyclodextrin, Anal. Chim. Acta., 394, p. 337 (1999).
[11] Su J.S., Long Y.F., Zhou M.F., Li R.T., UV-Visible Spectrophotometry Study on the Interaction of Brilliant Green with Deoxyribonucleic Acid and Application to DNA Assay, Chinese Journal of Spectroscopy Laboratory, 21, p. 160 (2004).
[12] Hamai S., Ohshida T., Inclusion Complexes of Cyclodextrins with Tetrakis(4-Carboxyphenyl) Porphyrin and Tetrakis(4-Sulfonatophenyl) Porphyrin in Aqueous Solutions, J. Inclusion Phenom. Macrocyclic Chem., 50, p. 209 (2004).
[13] Xi P.X., Xu Z.H., Liu X.H., Chen F.J., Zeng Z.Z., Zhang X.W. et al., Synthesis, Characterization, Antioxidant Activity and DNA-Binding Studies of Three Rare Earth (III) Complexes with 1-(4-Aminoantipyrine)-3-Tosylurea Ligand, J.Fluoresc, 19, p. 63 (2009).
[14] Ouameur A.A., Marty R., Tajmir-Riahi H.A., Human Serum Albumin Complexes with Chlorophyll and Chlorophyllin, Biopolymers, 77, p. 129 (2005).
[15] Priyadarsini K.I., Mohan H., Tyagi A.K., Mittal J.P., Inclusion Complex of g-Cyclodextrin-C60: Formation, Characterization, and Photophysical Properties in Aqueous Solutions, J. Phys. Chem., 98, p. 4756 (1994).
[16] Wang H.Y., Han J., Feng X.G., Pang Y.L., Study of Inclusion Complex Formation Between Tropaeolin OO and g-cyclodextrin by Spectrophotometry and Infrared Spectroscopy, Spectrochim. Acta, Part A., 65, p. 100 (20060.
[17] Xu D.L., Wang X.M., Fei D., Ding L.S., Study on the Interaction Between the Inclusion Complex of Hematoxylin with β-Cyclodextrin and DNA. Nucleosides, Nucleotides, Nucleic Acids., 29 , p. 854 (2010).
[18] Li H.B., Tuo H.G., Wang X.M., Wang S.Q., Ding L.S., Study on Interaction Between Hematoporphyrin Dihydrochloride and Herring Sperm DNA by Spectroscopy, Acta. Opt. Sin., 10, p. 2015 (2008).
[19] Ross P.D., Subramanian S., Thermodynamics of Protein Association Reactions: Forces Contributing to Stability, Biochemistry, 20, p. 3096 (1981).
[20] Mansuri-Torshizi H., Mital R., Srivastava1 T.S., Parekh H., Chitnis M.P., Synthesis, Characterization, and Cytotoxic Studies of α-Diimine/1,2-Diamine Platinum(II) and Palladium(II) Complexes of Selenite and Tellurite and Binding of Some of These Complexes to DNA, J. Inorg. Biochem., 44, p. 239 (1991).
[21] Lerman L.S., Structural Considerations in the Interaction of DNA and Acridines, J. Mol. Biol., 3, p. 18 (1961). 

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