Studies on the Binding of DNA with the Inclusion of Brilliant Green inside the Cavity of γ-Cyclodextrin

Document Type: Research Article

Authors

Department of Chemistry, College of Material Science and Engineering, Southwest University of Science and Technology, Mianyang, Sichuan 621010, CHINA

Abstract

The interaction of brilliant green with herring sperm DNA was investigated in detail by spectrometric methods in γ-cyclodextrin systems. On the condition of physiological pH, brilliant green prefers to form the 1:1 inclusion complex with γ-cyclodextrin.All the evidences indicated that the binding modes between γ-cyclodextrin-brilliant green and DNA were grooving binding and partial non-classical intercalative binding. The binding ratio of the inclusion complex with DNA is 6:1. The calculated thermodynamic parameters suggested that the binding of the inclusion complex to DNA was driven mainly by entropy.

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