The Effect of Instantaneous and Slow-Release Salt Stress Methods on Beta-Carotene Production within Dunaliella Salina Cells

Document Type : Research Article

Authors

1 Department of Chemical Engineering, Science and Research Branch, Islamic Azad University, Tehran, I.R. IRAN

2 Department of Chemical Engineering, Faculty of Engineering, University of Bojnord, Bojnord, I.R. IRAN

Abstract

The main concept of the present study is taken from the growth pattern of Dunaliella salina (green unicellular eukaryote microalgae) in Urmia Lake which is the second biggest saline lake in the world. In this study, different models of salt stress, including instantaneous (1 and 2 M) and consecutive slow-release salt stresses (0.5 M) were tested, and the amount of beta-carotene for each test was evaluated. According to the results, the highest amount of beta-carotene was obtained by the slow release method of salt injection with the amount of 9.01 µg of beta-carotene per mg of the dry weight of microalgae. The largest amount of beta-carotene production in 1 and 2 M  instantaneous salt stresses method were recorded as 4.35 and 0.65 µg/mg respectively which were up to 2 and 14 times lower than the highest beta-carotene production under the slow-release salt stress method.

Keywords

Main Subjects

References

[1] Jin E.S., Melis A., Microalgal Biotechnology: Carotenoid Production by the Green Algae Dunaliella salina, Biotechnol. Bioprocess. Eng., 8(6): 331 (2003).
[2] Azizi S., Hashemi A., Pajoum Shariati F., Bonakdarpour B., Safamirzaei M., Fouling Identification in Reciprocal Membrane Photobioreactor (RMPBR) Containing Chlorella vulgaris Species: Hydraulic Resistances Assessment, J. Chem. Technol. Biotechnol., (2020),
        doi: 10.1002/jctb.6552
[3] Ben-Amotz A., Glycerol Production in the Alga Dunaliella. Biochemical and Photosynthetic Aspects of Energy Production, 191-208 (1980).
[4] Mata, T.M., Martins A.A., Caetano N.S., Microalgae for Biodiesel Production and Other Applications: A Review, Renew. Sust. Energ. Rev., 14(1): 217-232 (2010).
[5] Hashemi A., Pajoum Shariati F., Sohani E., Azizi S., Hosseinifar S.Z., Delavari Amrei H., CO2 Biofixation By Synechococcus Elongatus from the Power Plant Flue Gas under Various Light–Dark Cycles. Clean. Technol. Envir., 1-9 (2020).
        doi: 10.1007/s10098-020-01912-0
[6] Azizi S., Bayat B., Tayebati H., Hashemi A., Pajoum Shariati F., Nitrate and Phosphate Removal from Treated Wastewater by Chlorella vulgaris under Various Light Regimes within Membrane Flat Plate Photobioreactor. Environ. Prog. Sustain. Energy., (2020).
        doi: 10.1002/ep.13519
[7] Hosseini M.K., Pajoum Shariati F., Hosseini P.K., Azizi S., Hashemi A., "The Effect of Polymeric Granule as Mechanical Cleaning Technology on Membrane Fouling in a Hybrid Microalgal Membrane Photobioreactor (HMPBR)." 6th MEMTEK International Symposium on Membrane Technologies and Applications., 18-20 November, Istanbul, Turkey (2019).  
[8] Rodolfi L., Zittelli G.C., Bassi N., Padovani G., Biondi N., Bonini G., Tredici M.R., Microalgae for Oil: Strain Selection, Induction of Lipid Synthesis and Outdoor Mass Cultivation in a Low‐Cost Photobioreactor, Biotechnol. Bioeng., 102(1): 100-112 (2009).
[9] Hosseini Tafreshi A., Shariati M., Dunaliella Biotechnology: Methods and Applications, J. Appl. Microbiol., 107(1): 14-35 (2009).
[10] Rasoul-Amini S., Ghasemi Y., Morowvat M.H., Mohagheghzadeh A.A., PCR Amplification of 18S rRNA, Single Cell Protein Production and Fatty Acid Evaluation of Some Naturally Isolated Microalgae. Food Chem., 116(1): 129-136 (2009).
[11] Ghasemi Y., Rasoul-Amini S., Morowvat M., Algae for the Production of SCP. Bioprocess Sciences and Technology. Nova Science Publishers, Inc,163-84 (2011).
[12] Borowitzka M.A., Dunaliella: Biology, Production, and Markets. Handbook of Microalgal Culture: Appl. Phycol. Biotechnol., 359-368 (2013).
[13] Murthy K.C., VanithaJ A., Rajesha J., MahadevaSwamy M., Sowmya P.R., Gokare A., Ravishankar., In vivo Antioxidant Activity of Carotenoids from Dunaliella salina—A Green Microalga. Life Sci., 76(12): 1381-1390 (2005).
[14] Leach G., Oliveira G., Morais r., Production of a Carotenoid‐Rich Product by Alginate Entrapment and Fluid‐Bed Drying of Dunaliella salina. J. Sci. Food. Agr., 76(2): 298-302 (1998).
[15]  Hashemi A., Pajoum Shariati F., Delavari Amrei H., Heydarinasab A., Growth Pattern and β-Carotene Production of Dunaliella salina Cells in Different Salinities, J. Food. Technol. Nutr, 16(4): 45-50 (2019).
[16] Del Campo J.A., García-González M., Guerrero M.G., Outdoor Cultivation of Microalgae for Carotenoid Production: Current State and Perspectives, Appl. Microbiol. Biot., 74(6): 1163-1174 (2007).
[17] Tanada T., The Photosynthetic Efficiency of Carotenoid Pigments in Navicula Minima, Am. J. Bot., 38(4): 276-283 (1951).
[18] Lers A., Biener Y., Zamir A., Photoinduction of Massive β-carotene Accumulation by the Alga Dunaliella bardawil: Kinetics and Dependence on Gene Activation, Plant Physiol., 93(2): 389-395 (1990).
[19] Hashemi A., Moslemi M., Pajoum Shariati F., Delavari Amrei H., Beta‐Carotene Production Within Dunaliella salina Cells under Salt Stress Condition in an Indoor Hybrid Helical‐Tubular Photobioreactor. Can. J. Chem. Eng, 98(1): 69-74 (2020).
[20] Delavari Amrei H., Nasernejad B., Ranjbar R., Rastegar S., An Integrated Wavelength-Shifting Strategy for Enhancement of Microalgal Growth Rate in PMMA-and Polycarbonate-Based Photobioreactors, Eur. J. Phycol., 49(3): 324-331 (2014).
[21] Leon R., Martın M., Vigara J., Vilchez C., Vega J.M., Microalgae Mediated Photoproduction of β-carotene in Aqueous–Organic Two Phase Systems, Biomol. Eng., 20(4-6): 177-182 (2003).
[22] Morowvat M.H., Ghasemi Y., Culture Medium Optimization for Enhanced β-carotene and Biomass Production by Dunaliella salina in Mixotrophic Culture, Biocatal. Agric Biotechnol., 7: 217-223 (2016).
[23] Tammam A.A., Fakhry E.M., El-Sheekh M., Effect of Salt Stress on Antioxidant System and the Metabolism of the Reactive Oxygen Species in Dunaliella salina and Dunaliella tertiolecta. Afr. J. Biotechnol., 10(19): 3795-3808 (2011).
[24] Hadi M., Shariati M., Afsharzadeh S., Microalgal Biotechnology: Carotenoid and Glycerol Production by the Green Algae Dunaliella Isolated from the Gave-Khooni Salt Marsh, Iran. Biotechnol. Bioprocess. Eng., 13(5): 540 (2008).
[25] García F., Freile-Pelegrín Y., Robledo D., Physiological Characterization of Dunaliella sp.(Chlorophyta, Volvocales) from Yucatan, Mexico. Bioresour. Technol., 98(7): 1359-1365 (2007).
[26] Fazeli M., Tofighi H., Samadi N., Jamalifar H.,  Effects of Salinity on β-carotene Production by Dunaliella tertiolecta DCCBC26 Isolated from the Urmia Salt Lake, North of Iran. Bioresour. Technol., 97(18): 2453-2456 (2006).
[27] Rao A.R., Dayananda C., Sarada R., Shamala T.R., Ravishankar G.A., Effect of Salinity on Growth of Green Alga Botryococcus Braunii and Its Constituents, Bioresour. Technol., 98(3): 560-564 (2007).
[28] Borowitzka M.A., The ‘Stress’ Concept in Microalgal Biology—Homeostasis, Acclimation and Adaptation, J. Appl. Phycol., 30(5): 2815-2825 (2018).
[29] Lakeman M.B., Von Dassow P., Cattolico R.A., The Strain Concept in Phytoplankton Ecology. Harmful. Algae., 8(5): 746-758 (2009).
[30] Galluzzi, L., Bravo-San Pedro J.M., Kepp O., Kroemer G., Regulated Cell Death and Adaptive Stress Responses, Cell. Mol. Life. Sci., 73(11-12): 2405-2410 (2016).
[31] Berges J.A., Falkowski P.G., Physiological Stress and Cell Death in Marine Phytoplankton: Induction of Proteases in Response to Nitrogen or Light Limitation, Limnol. Oceanogr., 43(1): 129-135 (1998).
[32] Timmermans K.R., Veldhuis M.J., Brussaard C.P., Cell Death in Three Marine Diatom Species in Response to Different Irradiance Levels, Silicate, or Iron Concentrations, Aquat. Microb. Ecol., 46(3): 253-261 (2007).
[33] Borowitzka M.A., Borowitzka L.J., Kessly D., Effects of Salinity Increase on Carotenoid Accumulation in the Green Alga Dunaliella salina, J. Appl. Phycol., 2(2): 111-119 (1990).
[34] Ginzburg M., Ginzburg B., Interrelationships of Light, Temperature, Sodium Chloride and Carbon Source in Growth of Halotolerant and Halophilic Strains of Dunaliella. Brit. Phycol. J., 16(3): 313-324 (1981).
[35] Pick U., Adaptation of the Halotolerant Alga Dunaliella to High Salinity, In "Salinity: Environment-Plants-Molecules", Springer 97-112 (2002).
[36] Kawasaki S., Borchert C., Deyholos M., Wang H., Brazille S., Kawai K., Galbraith D., Bohnert H.J., Gene Expression Profiles During the Initial Phase of Salt Stress in Rice. The Plant Cell., 13(4): 889-905 (2001).
[37] Borowitzka M.A., Siva C.J., The Taxonomy of the Genus Dunaliella (Chlorophyta, Dunaliellales) with Emphasis on the Marine and Halophilic Species, J. Appl. Phycol., 19(5): 567-590 (2007).
[38] Borowitzka M., Huisman J., The Ecology of Dunaliella salina (Chlorophyceae, Volvocales): Effect of Environmental Conditions on Aplanospore Formation. Bot. Mar., 36(3): 233-244 (1993).
[39] Nedelcu A.M., Sex as a Response to Oxidative Stress: Stress Genes Co-Dopted for Sex, Proceedings of the Royal Society of London B: Biolog. Sci., 272(1575): 1935-1940 (2005).
[40] Bravo I., Figueroa R.I., Towards an Ecological Understanding of Dinoflagellate Cyst Functions. Microorganisms., 2(1): 11-32 (2014).
[41] Ben‐Amotz A., Katz A., Avron M., Accumulation of β‐carotene in Halotolerant Alge: Purification and Characterization of β‐Barotene-Rich Globules from Dunaliella bardawil (Chlorophyceae) 1, J. Phycol., 18(4): 529-537 (1982).
[42] Lesser M.P., Oxidative Stress in Marine Environments: Biochemistry and Physiological Ecology, Annu. Rev. Physiol., 68: 253-278 (2006).
[43] Leshem Y., Seri L., Levine A., Induction of Phosphatidylinositol 3‐kinase‐Mediated Endocytosis by Salt Stress Leads to Intracellular Production of Reactive Oxygen Species and Salt Tolerance, The Plant Journal., 51(2): 185-197 (2007).
[44] Malanga G., Calmanovici G., Puntarulo S., Oxidative Damage to Chloroplasts from Chlorella vulgaris Exposed to Ultraviolet‐B Radiation, Physiol Plantarum., 101(3): 455-462 (1997).
[45] El Baz F.K., Aboul-Enein A.M., El-Baroty G.S., Youssef A.M., Abdel-Baky H.H., Accumulation of Antioxidant Vitamins in Dunaliella salina, (2002).
[46] Weiss M., Pick U., Transient Na+ Flux Following Hyperosmotic Shock in the Halotolerant Alga Dunaliella salina: A Response to Intracellular pH Changes, J. plant Physiol., 136(4): 429-438 (1990).
[47] Katz A., Pick U., Avron M., Modulation of Na+/H+ Antiporter Activity by Extreme pH and Salt in the Halotolerant Alga Dunaliella salina, Plant physiol., 100(3): 1224-1229 (1992).
[48] Karni L., Avron M., Ion Content of the Halotolerant Alga Dunaliella salina, Plant Cell Physiol., 29(8): 1311-1314 (1988).
[49] Issa A., The Role of Calcium in the Stress Response of the Halotolerant Green Alga Dunaliella bardawi,  Phyton (Horn, Austria), 36: 295-302 (1996).
[50] Bickerton P., Sello S., Brownlee C., Pittman J.K., Wheeler G.L., Spatial and Temporal Specificity of Ca2+ Signalling in Chlamydomonas reinhardtii in Response to Osmotic Stress, New Phytol., 212(4): 920-933 (2016).
[51] Cifuentes A., Gonzalez M.A., Parra O.O., The Effect of Salinity on the Growth and Carotenogenesis in Two Chilean Strains of Dunaliella salina Teodoresco, Biol. Res., 29: 227-236 (1996).
[52] Gomez P.I., Barriga A., Cifuentes A.S., González M.A., Effect of Salinity on the Quantity and Quality of Carotenoids Accumulated by Dunaliella salina (Strain CONC-007) and Dunaliella bardawil (Strain ATCC 30861) Chlorophyta. Biol. Res., 36(2): 185-192 (2003).
[53] Moulton T., Burford M., The Mass Culture of Dunaliella viridis (Volvocales, Chlorophyta) for Oxygenated Carotenoids: Laboratory and Pilot Plant Studies, Thirteenth International Seaweed Symposium; Springer (1990).
[54] Cifuentes A.S., González M., Conejeros M., Dellarossa V., Parra O., Growth and Carotenogenesis in Eight Strains of Dunaliella salina Teodoresco from Chile, J. Appl. Phycol., 4(2): 111 (1992).
Iranian Journal of Chemistry and Chemical Engineering
Volume 40, Issue 5 - Serial Number 109
September and October 2021
Pages 1642-1652

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