Impact of Chitosan-Capric Acid Nanogels Incorporating Thyme Essential Oil on Stability of Pomegranate Seed Oil-in-Water Pickering Emulsion

Document Type : Research Article

Authors

1 Department of Food Science, School of Agricultural Engineering, Shahrood University of Technology, Shahrood, I.R. IRAN

2 Microbial Biotechnology Department, Agricultural Biotechnology Institute of Iran (ABRII), Agricultural Research, Education, and Extension Organization (AREEO), 31535-1897 Karaj, I.R. IRAN

3 Nanosystems Research Team (NRTeam), Karaj, I.R. IRAN

4 Research & Development Department of Nanozino, Tehran, I.R. IRAN

5 Department of Horticulture and Plant Protection, School of Agricultural Engineering, Shahrood University of Technology, Shahrood, I.R. IRAN

Abstract

The aim of this study was to obtain a stable Pomegranate Seed Oil (PSO)-in-water Pickering emulsion stabilized by chitosan (CS)-capric acid (CA) nanogels incorporating Thyme Essential Oil (TEO). Firstly, CS-CA nanogels were synthesized at different ratios of CA to CS (0.25:1, 0.5:1, and 0.75:1). Scanning electron microscopy images showed that by increasing the CA to CS ratio, the uniformity of particles was increased. In the following, CS-CA nanogels were used to stabilize PSO-in-water emulsions. The findings revealed that the most stable emulsion was obtained at pH 8, CA-to-CS ratio of 0.5:1, and an oil-to-nanogel ratio of 10:1. In addition, the interfacial structure of emulsion droplets indicated that the CS-CA nanogels contributed to the stability of emulsion through both the formation of an interface layer and a network on the surface of dispersed droplets. Finally, the oxidative stability and microstructure of the emulsions stabilized by CS-CA nanogels incorporating TEO (0.1%) were evaluated. The results showed that TEO increased the oxidative stability of the emulsion and reduced the emulsion droplet size.

Keywords

Main Subjects


[1] Mark-herbert C., Innovation of a New Product Category -Functional Foods, Technovation., 24: 713–9 (2004).
[2] Yazicioglu B., Sahin S., Sumnu G.,  Microencapsulation of Wheat Germ Oil, J. Food Sci. Technol., 52:3590–3597 (2015).
[3] Soleimanian Y., Goli S.A.H., Varshosaz J., Sahafi S.M., Formulation and Characterization of Novel Nanostructured Lipid Carriers Made from Beeswax, Propolis Wax and Pomegranate Seed Oil, Food Chem., 244: 83–92 (2018).
[4] Özcan M.M., Aljuhaimi F., Uslu N., Mohamed Ahmed I.A., Osman M.A., Gassem M.A., Salih H.A., Effect of Oven Drying on Antioxidant Activity, Phenolic Compounds, Fatty Acid Composition and Tocopherol Contents of Pomegranate Aril and Oils, J. Food Process Preserv., 43: e13885 (2019).
[5] Ojagh S.M., Hasani S., Characteristics and Oxidative Stability of Fish Oil Nano-Liposomes and Its Application in Functional Bread, J. Food Meas. Charact., 12: 1084–1092 (2018).
[7] Atarian M., Rajaei A., Tabatabaei M., Mohsenifar A., Bodaghi H., Formulation of Pickering Sunflower Oil-in-Water Emulsion Stabilized by Chitosan-Stearic Acid Nanogel and Studying its Oxidative Stability. Carbohydr Polym., 210: 47–55 (2019).
[8] Rajaei A., Hadian M., Mohsenifar A., Rahmani-Cherati T., Tabatabaei M., A coating Based on Clove Essential Oils Encapsulated by Chitosan-Myristic Acid Nanogel Efficiently Enhanced the Shelf-Life of Beef Cutlets. Food Package Shelf Life., 14: 137–145 (2017).
[9] Chen S., Han Y., Jian L., Liao W., Zhang Y., Gao Y., Fabrication, Characterization, Physicochemical Stability of Zein-Chitosan Nanocomplex for Co-Encapsulating Curcumin and Resveratrol, Carbohydr. Polym., 236: 116090 (2020).
[10] Liu K., Huang R-L., Zha X.-Q., Li Q-M., Pan L-H., Luo J-P., Encapsulation and Sustained Release of Curcumin by a Composite Hydrogel of Lotus Root Amylopectin and Chitosan, Carbohydr Polym., 232: 115810 (2020).
[11] Wang Y., Khan A., Liu Y., Feng J., Dai L., Wang G., Alam N., Tong L., Ni Y.,  Chitosan Oligosaccharide-Based Dual pH Responsive Nano-Micelles for Targeted Delivery of Hydrophobic Drugs, Carbohydr Polym., 223: 115061 (2019).
[14] Abbasian M., Synthesis and Characterization of Polyaniline-polystyrene-chitosan/zinc Oxide Hybrid Nanocomposite. Iran. J. Chem. Chem. Eng. (IJCCE), 38: 55-64 (2018).
[15] Qayyum S., Mehmood M., Mirza M.A., Ashraf S.,  Ahmed Z., Tanvir T., Choudhary M.A., Iqbal M., Nisar F., Nisa Z., Synthesis and Characterization of Silver and Gold Nano-Structures on Chitosan-Porous Anodic Alumina Nano-Composite. Iran. J. Chem. Chem. Eng. (IJCCE), 38: 31-44 (2018).
[19] Ou H., Chen Q., Pan J., Zhang Y., Huang Y., Qi X., Selective Removal of Erythromycin by Magnetic Imprinted Polymers Synthesized from Chitosan-Stabilized Pickering Emulsion, J. Hazard. Mater., 289: 28–37 (2015).
[20] Elsabee M.Z., Morsi R.E., Al-Sabagh A.M., Surface Active Properties of Chitosan and Its Derivatives. Colloids Surfaces B Biointerfaces., 74: 1–16 (2009).
[21] Zhang S., Zhou Y., Yang C., Pickering Emulsions Stabilized by the Complex of Polystyrene Particles and Chitosan, Colloids Surfaces A Physicochem. Eng. Asp., 482:338–44 (2015).
[22] Bakkali F., Averbeck S., Averbeck D., Idaomar M., Biological Effects of Essential Oils-A Review, Food Chem. Toxicol., 46: 446–475 (2008).
[23] Nakhaee Moghadam M., Movaffagh J., Fazli Bazzaz B.S., Azizzadeh M., Jamshidi A., Encapsulation of Zataria multiflora Essential Oil in Saccharomyces cerevisiae: Sensory Evaluation and Antibacterial Activity in Commercial Soup, Iran. J. Chem. Chem. Eng. (IJCCE), 39(2): 233-242 (2019).
[24] Ayari J., Karoui I.J., Abderrabba M., A Comparative Study between Different Tunisian Propolis Essential Oils and Their Antioxidant Activities, Iran. J. Chem. Chem. Eng. (IJCCE), 39: 215-229 (2020).
[25] Raeisi M., Hashami M., Afshari A., Tabarraei A., Aminzare M., Jannat B., Cinnamon and Rosemary Essential Oils Incorporated into Alginate Coating Improve Chemical and Sensorial Quality of Chicken Meat, Iran. J. Chem. Chem. Eng. (IJCCE), 38: 293-304 (2019).‏
[27] Gouda M., Zhang S., Liu Y., Sheng L., Ma M., Effects of Four Natural Antioxidant Phenyl
Terpenes on Emulsifying and Rheological Properties of Egg Yolk
, LWT-Food Sci Technol., 83: 59–67 (2017).
[28] Lei L., He Z., Chen H., McClements D.J, Li B., Li Y., Microstructural, Rheological, and Antibacterial Properties of Cross-linked Chitosan Emulgels, RSC Adv., 5: 100114–22 (2015).
[29] Chen E., Wu S., McClements D.J., Li B., Li Y., Influence of pH and Cinnamaldehyde on the Physical Stability and Lipolysis of Whey Protein Isolate-Stabilized Emulsions, Food Hydrocoll., 69: 103–10 (2017).
[31] Yang Y., Song X., Sui X., Qi B., Wang Z., Li Y.,
Jiang L., Rosemary Extract Can Be Used as a Synthetic Antioxidant to Improve Vegetable Oil Oxidative Stability, Ind Crops Prod., 80: 141–147 (2016).
[32] Paunov V.N., Cayre O.J., Noble P.F., Stoyanov S.D., Velikov K.P., Golding M., Emulsions Stabilised by Food Colloid Particles: Role of Particle Adsorption and Wettability at the Liquid Interface, J. Colloid Interface Sci., 312: 381–389 (2007).
[34] Huang Y., Cai Y., Lapitsky Y., Factors Affecting the Stability of Chitosan/tripolyphosphate Micro-and Nanogels: Resolving the Opposing Findings, J. Mater. Chem B., 3: 5957–5970 (2015).
[36] Salminen H., Aulbach S., Leuenberger B.H., Tedeschi C., Weiss J., Influence of Surfactant Composition on Physical and Oxidative Stability of Quillaja Saponin-Stabilized Lipid Particles with Encapsulated ω-3 Fish Oil, Colloids Surfaces B Biointerfaces., 122: 46–55 (2014).
[38] Mwangi W.W., Ho K.W., Tey B.T., Chan E.S., Effects of Environmental Factors on the Physical Stability of Pickering-Emulsions Stabilized by Chitosan Particles, Food Hydrocoll., 60: 543–550 (2016).
[39] Nan F., Wu J., Qi F., Liu Y., Ngai T., Ma G., Uniform Chitosan-coated Alginate Particles as Emulsifiers for Preparation of Stable Pickering Emulsions with Stimulus Dependence, Colloids Surfaces A Physicochem Eng. Asp., 456: 246–252 (2014).
[40] Xiao J., Wang X., Perez Gonzalez A.J., Huang Q., Kafirin Nanoparticles-Stabilized Pickering Emulsions: Microstructure and Rheological Behavior, Food Hydrocoll., 54: 30–39 (2016).
[41] Shah B.R., Li Y., Jin W., An Y., He L., Li Z., Xu W., Li B., Preparation and Optimization of Pickering Emulsion Stabilized by Chitosan-tripolyphosphate Nanoparticles for Curcumin Encapsulation, Food Hydrocoll., 52: 369–377 (2016).
[42] Wang L.J., Hu Y.Q., Yin S.W., Yang X.Q., Lai F.R., Wang S.Q., Fabrication and Characterization of Antioxidant Pickering Emulsions Stabilized by Zein/Chitosan Complex Particles (ZCPs), J Agric. Food Chem., 63: 2514–2524 (2015)
[43] Dickinson E., Food Emulsions and Foams: Stabilization by Particles, Curr. Opin. Colloid Interface Sci., 15:40-49 (2010).
[44] Nakaya K., Ushio H., Matsukawa S., Shimizu M., Ohshima T., Effects of Droplet Size on the Oxidative Stability of Oil-in-Water Emulsions, Lipids., 40: 501–507 (2005).