Submicron Particles of Double Network Alginate/Polyacrylamide Hydrogels for Drug Delivery of 5-Fluorouracil

Document Type : Research Article

Authors

Department of Chemistry, Payam Noor University (PNU), Tehran, I.R. IRAN

Abstract

The purpose of this study was to prepare a Double Network (DN) hydrogel made with sodium-alginate (Na-alg) natural polysaccharide and polyacrylamide (PAM) chains that target control release of anticancer drug. To this aim, the entitled submicron particles of double network hydrogels exhibit exceptional properties. A two-step strategy was used to obtain alginate/PAM hydrogels crosslinked by Ca2+ cations and N, Nʼ-methylenebisacrylamide (MBA) as different types of crosslinker. Particles of DN alginate / PAM were employed for entrapping and releasing the model drug 5-fluorouracil (5-FU) as an anticancer drug. Scanning Electron Microscopy (SEM), Fourier Transform InfraRed (FT-IR) spectroscopy, ThermoGravimetric Analysis (TGA), Dynamic Light Scattering (DLS), swelling properties, drug entrapment efficiency, and drug release studies were also done. The particles showed porous structure, good swelling ability, and aqueous dispersibility with a size in the range of 280 nm. The resulting hydrogel was subsequently loaded with 5-FU and released patterns carried in 7.4 pH and 5.8 pH at a temperature of 37 ºC. The controlled drug release behavior was noticed and the finding of the study suggested that the alginate/PAM double network hydrogel is a promising carrier for 5-FU delivery.

Keywords

Main Subjects

References

[1] Almaki J.H., Nasiri R., Idris A., Nasiri M., Majid F.A.A., Losic D., Trastuzumab-Decorated Nanoparticles for in Vitro And In Vivo Tumor-Targeting Hyperthermia of HER2+ Breast Cancer, J. Mater. Chem. B. (J.M.C.B.), 5(35): 7369–7383 (2017).
[2] Peer D., Karp J.M., Hong S., Farokhzad O.C., Margalit R., Langer R., Nanocarriers as an Emerging Platform for Cancer Therapy, Nat. Nanotechnol.(N.N.), 2(12): 751–760 (2007).
[3] Lohcharoenkal W., Wang L., Chen YC., Rojanasakul Y., Protein Nanoparticles as Drug Delivery Carriers for Cancer Therapy, BioMed. Research. International. (B.R.I.), 2014: 1-12 (2014).
[4] Nuttelman C.R., Rice M.A., Rydholm A.E., Salinas C.N., Shah D.N., Anseth K.S., Macromolecular Monomers for the Synthesis of Hydrogel Niches and their Application in Cell Encapsulation and Tissue Engineering, Prog. Polym. Sci. (P.P.S.), 33(2): 167–179 (2008).
[5] Lin C.C., Metters A.T., Hydrogels in Controlled Release Formulations: Network Design and Mathematical Modeling, Adv. Drug. Deliv. Rev. (ADDR), 58(12): 1379–408 (2006).
[6] Langer R., Tirrell D.A., Designing Materials for Biology and Medicine, Nature, 428(6982): 487–492 (2004).
[7] Gong J.P., Why Are Double Network Hydrogels so Tough?, Soft. Matter. (S.F.), 6(12) :2583–2990 (2010).
[8] Sakai T., Matsunaga T., Yamamoto Y., Ito C., Yoshida R., Suzuki S., Sasaki N., Shibayama M., Chung U.-i., Design and Fabrication of a High-Strength Hydrogel with Ideally Homogeneous Network Structure from Tetrahedron-like Macromonomers, Macromolecules, 41(14): 5379–5384 (2008).
[9] Haque Md.A., Kurokawa T., Gong J.P., Super Tough Double Network Hydrogels and their Application as Biomaterials, Polymer. 53(9): 1805–1822 (2012).
[10] Li C., Rowland M.J., Shao Y., Cao T., Chen C., Jia H., Zhou X., Yang Z., Scherman O.A., Liu D., Responsive Double Network Hydrogels of Interpenetrating DNA and CB[8] Host–Guest Supramolecular Systems, Adv. Mater.(A.M.), 27(21): 3298–3304 (2015).
[11] Wu Z.L., Kurokawa T., Gong J.P., Hydrogels with a Macroscopic-Scale Liquid Crystal Structure by Self-Assembly of a Semi-Rigid Polyion Complex, Polym. J.(PJ), 44(6): 503–511 (2012).
[12] Rokhade A.P., Agnihotri S.A., Patil S.A., Mallikarjuna N.N., Kulkarni P.V., Aminabhavi T.M., Semi-Interpenetrating Polymer Network Microspheres of Gelatin and Sodium Carboxymethyl Cellulose for Controlled Release of Ketorolac Tromethamine, Carbohydr. Polym.(CP), 65(3): 243–252 (2006).
[13] Minhas M.U., Ahmad M., Ali L., Sohail M., Synthesis of Chemically Cross-Linked Polyvinyl Alcohol-co-poly (Methacrylic Acid) Hydrogels by Copolymerization; a Potential Graft-Polymeric Carrier for Oral Delivery of 5-Fluorouracil, DARU J Pharm Sci. (DJPS), 21(1): 44-53 (2013).
[14] Ailincai D., Tartau M.L., Marin L., Drug Delivery Systems Based on Biocompatible Imino-Chitosan Hydrogels for Local Anticancer Therapy, Drug Deliv.(D.D.), 25(1): 1080-1090 (2018).
[15] Vinod B.S., Antony J., Nair H.H., Puliyappadamba V.T., Saikia M., Narayanan S.S., Bevin A., Anto R.J., Mechanistic Evaluation of the Signaling Events Regulating Curcumin-Mediated Chemosensitization of Breast Cancer Cells to 5-Fluorouracil, Cell. Death. Dis. (C.D.D.), 4(2): 505–518 (2013).
[16] Milczarek M., Wiktorska K., Mielczarek L., Koronkiewicz M., Dąbrowska A., Lubelska K.,Matosiuk D., Chilmonczyk Z., Autophagic cell Death and Premature Senescence: New Mechanism of 5-Fluorouracil and Sulforaphane Synergistic Anticancer Effect in MDA-MB-231 Triple Negative Breast Cancer Cell Line, Food. Chem. Toxicol.(F.C.T.), 111: 1–8 (2018).
[17] Singh B., Chauhan N., Preliminary Evaluation of Molecular Imprinting of 5-Fluorouracil within Hydrogels for Use as Drug Delivery Systems, Acta Biomater.(A.B.), 4(5): 1244–1254.
[18] Rao K.M., Mallikarjuna B., Rao K.S.V.K., Sudhakar K., Rao K.C., Subha M.C.S., Synthesis and Characterization of pH-Sensitive Poly (Hydroxy Ethyl Methacrylate-co-acrylamidoglycolic Acid) Based Hydrogels for Controlled Release Studies of 5-fluorouracil. Int. J. Polym. Mater. Polym. Biomater. (I.J.P.M.P.B.), 62(11): 565–571 (2013).
[19] Rokhade A.P., Shelke N.B., Patil S.A., Aminabhavi T.M., Novel Hydrogel Microspheres of Chitosan and Pluronic F-127 for Controlled Release of 5-Fluorouracil, J. Microencapsul. (J.M.), 24(3): 274–388 (2007).
[20] Baumberger T., Ronsin O., Cooperative Effect of Stress and Ion Displacement on the Dynamics of Cross-Link Unzipping and Rupture of Alginate Gels. Biomacromolecules.11(6): 1571–8 (2010).
[21] Adzmi F., Meon S., Musa M.H., Yusuf N.A., Preparation, Characterisation and Viability of Encapsulated Trichoderma Harzianum UPM40 in Alginate-Montmorillonite Clay, J. Microencapsul. (JM), 29(3): 205–210 (2012).
[22] Sartori C., Finch D.S., Ralph B., Gilding K., Determination of the Cation Content of Alginate Thin Films by FTi.r. Spectroscopy, Polymer, 38(1): 43–51 (1997).
[23] Jain R., Mahto V., Evaluation of Polyacrylamide/Clay Composite as a Potential Drilling Fluid Additive in Inhibitive Water Based Drilling Fluid System, J. Pet. Sci. Eng. (J.P.S.E.), 133: 612–621 (2015).
[24] Patel D., Patel C., Jani R., Design andEvaluation of Colon Targeted Modified Pulsincap Delivery of 5-fluorouracil According to Circadian Rhythm, Int. J. Pharm. Investig. (I.J.P.I.), 1(3): 172-181 (2011).
[25] Parhi P., Ramanan A., Ray A., Preparation and Characterization of Alginate and Hydroxyapatite‐Based Biocomposite, J. Appl. Polym. Sci. (J.A.P.), 102: 5162–5165 (2006).
[26] Soppimath K.S., Aminabhavi T.M., Dave A.M., Kumbar S.G., Rudzinski W.E., Stimulus-Responsive “Smart” Hydrogels as Novel Drug Delivery Systems, Drug. Dev. Ind. Pharm. (D.D.I.P.), 28(8): 957–974 (2002).
[27] Korsmeyer R.W., Peppas N.A., Effect of the Morphology of Hydrophilic Polymeric Matrices on the Diffusion and Release of Water Soluble Drugs, J. Membr. Sci. (J.M.S.), 9(3): 211–227 (1981).
[28] Samanta H.S., Ray S.K., Synthesis, Characterization, Swelling and Drug Release Behavior of Semi-Interpenetrating Network Hydrogels of Sodium Alginate and Polyacrylamide, Carbohydr Polym. (C.P.), 99: 666-678 (2014).
[29] Hoare T.R., Kohane D.S., Hydrogels in Drug Delivery: Progress and Challenges, Polymer, 49(8): 1993–2007 (2008).
[30] Ulbrich K., Šubr V., Polymeric Anticancer Drugs with Ph-Controlled Activation, Adv. Drug. Deliv. Rev. (A.D.D.R.), 56(7): 1023-1050 (2004).
[31] Ritger P.L., Peppas N.A., A Simple Equation for Description of Solute Release II. Fickian and Anomalous Release from Swellable Devices, J. Controlled. Release. (J.C.R.), 5(1): 37-42 (1987).
Iranian Journal of Chemistry and Chemical Engineering
Volume 40, Issue 5 - Serial Number 109
September and October 2021
Pages 1386-1394

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