The Effect of Sulfobetaine on the Activity of Strobilurins and Benzimidazole Fungicides

Document Type : Research Article


1 Department of Technology and Instrumental Analysis, Poznan University of Economics and Business, al. Niepodległości 10, Poznan 61-875, POLAND

2 Institute of Plant Protection, National Research Institute, ul. Węgorka 20, Poznan 60-318, POLAND



Surfactants (adjuvants) are the substances often added to the spray tank, besides the pesticide formulation, whose role is to improve the performance of the pesticide or the physical properties of the spray mixture, or both. Adjuvants are not often used as fungicides themselves, however, it is interesting to investigate if they can improve the fungistatic activity of the system they are included in. Therefore the main aim of this work was to determine the effect of the addition of a newly obtained, never investigated before, sulfobetaine type surfactant to the fungicides on their final properties. In the in vitro experiment fungistatic activity of the surfactant and the system of surfactant with strobilurins and benzimidazole fungicides were evaluated. The tested surfactants were added to the liquid medium of Potato Dextrose Agar (PDA) at concentrations: 1, 10, 50, and 100 ppm. The organisms of Fusarium culmorum, Rhizoctonia solani, Microdochium nivale, and Alternaria brassicas were used as indicators. The experiments have shown that the addition of the new surfactant studied to the medium resulted in inhibition of mycelium growth of all the tested fungi. The system with the surfactant and the fungicide was much more effective against the tested fungi than individual fungicides.


Main Subjects

[1] Rinella J.V., Workman R.F., Hermodson M.A., White J.L., Hem S.L., Elutability of Proteins from Aluminum-Containing Vaccine Adjuvants by Treatment with Surfactants, Journal of Colloid and Interface Science, 197:48-56 (1998).
[2] Heremans B., Isebaert S., Verhorven R., Ryckaert B., Spanoghe P., Steurbaut W., Haesaert G., “Effect of Adjuvants on the Efficiency of Dimethomorph Plus Mancozeb (Acrobat 2 Kg/Ha) on the Control of Late Blight In Potato”, Presented at the Proceedings of the Tenth Workshop of an European Network for Development of an Integrated Control Strategy of Potato Late Blight, Bologna (Italy), 269-274 (2008).
[3] Van Zyl S.A., Brink J.C., Calitz F.J., Coertze S., Fourie P.H., The Use of Adjuvants to Improve Spray Deposition and Botrytis Cinerea Control on Chardonnay Grapevine Leaves, Crop Protection, 29: 58-67 (2010).
[4] Gimenes M.J., Zhu H., Raetano C.G., Oliveira R.B., Dispersion and Evaporation of Droplets Amended with Adjuvants on Soybeans, Crop Protection, 44: 84-90 (2013).
[5] Van Zyl S.A., Brink J.C., Calitz F.J., Fourie P.H., Effects of Adjuvants on Deposition Efficiency of Fenhexamid Sprays Applied to Chardonnay Grapevine Foliage, Crop Protection, 29: 843-852 (2010).
[6] Karolewski Z., Wachowiak M., Ratajkiewicz H., Kierzek R., Effect of Adjuvants, Spray Volume and Nozzle Type on Metconazole Activity Against Leptosphaeria Biglobosa and L. Maculans During Late Spring Treatments in Winter Oilseed Rape, Journal of Plant Protection Research, 49: 113-118 (2009).
[7] Nganje I.B., Bangsund D.A., Leistritz F.L., Wilson W.W., Tiapo NM.,” Estimating the Economic Impact of Crop Disease: the Case of Fusarium Head Blight in U.S. Wheat and Barley”, In: National Fusariul Head Blight Forum Proceedings, East Lansing, Michigan State University, 275-281 (2002).
[8] Parry DW., Jenkinson P., Mcleod L., Fusarium Ear Blight (Scab) in Small Grain Cereals-A Review, Plant Pathology, 44: 207-238 (1995).
[10] Trail F., Xu J.R., San Miguel P., Halgren R.G., Kistler HC., Analysis of Expressed Sequence Tags from Gibberella Zeae (Anamorph Fusarium Graminearum), Fungal Genetics and Biology, 38: 187-197 (2003).
[11] Chahal G.S., Jordan D.L., Shew B.B., Brandenburg R.L., Burton J.D., Danehower D., York A.C., Interactions of Agrochemicals Applied to Peanut, Part 2: Effects on Fungicides, Crop Protection, 41: 143-149 (2012).
[13] Walkiewicz F., Materna K., Kropacz A., Michalczyk A., Gwiazdowski R., Praczyk T., Pernak J., Multifunctional Long-Alkyl-Chain Quaternary Ammonium Azolate Based Ionic Liquids, New Journal of Chemisty, 34: 2281-2289 (2010).
[14] Markiewicz B., Łęgosz B., Kordala-Markiewicz R., Wakiewicz F., Gwiazdowski R., Kubiak K., Praczyk T., Tebuconazole and Propiconazole Salts, Przemysł Chemiczny, 92: 1636-1639 (2013).
[15] Qu G., Cheng J., Wei J., Yu T., Ding W., Luan H., Synthesis, Characterization and Surface Properties of Series Sulfobetaine Surfactants, Journal of Surfactants and Detergents, 14: 31-35 (2011).
[16] Wieczorek D., Staszak K., Surface Activity of Selected Sulfobetaines, Polish Journal of Commodity Science, 36: 122-130 (2013).
[17] Wieczorek D., Gwiazdowska D., Staszak K., Chen Y., Shen T., Surface and Antimicrobial Activity of Sulfobetaines, Journal of Surfactants and Detergents, 19: 813–822 (2016). 
[18] Cheng C., Qu G., Wei J., Yu T., Ding W., Thermodynamics of Micellization of Sulfobetaine Surfactants in Aqueous Solution, Journal of Surfactants and Detergents, 15: 757-763 (2012).
[19] Glen K., Boligłowa E., Evaluation of the Fungistatic Activity of Plant Extracts In Vitro Tests, Journal of Research and Applications in Agricultural Engineering, 57: 104-109 (2012).
[20] Khan M.F.R., Khan G.R.J., Harikrishnan R., Nelson R., Bradley C.A., Effect of Adjuvants on the Performance of Pyraclostrobin for Controlling Cercospora Leaf Spot on Sugarbeet, Journal of Sugar Beet Research,44: 71-81 (2007).
[21] Gent D.H., Schwartz H.F., Nissen S.J., Effect of Commercial Adjuvants on Vegetable Crop Fungicide Coverage, Absorption, and Efficacy, Plant Disease, 87: 591-597 (2003).