Production and Solubility of Ectoine: Biochemical and Molecular Dynamics Simulation Studies

Document Type : Research Article

Authors

1 Department of Chemical Engineering, Faculty of Engineering, North Tehran Branch, Islamic Azad University, Tehran, I.R. IRAN

2 Department of Environment, Faculty of Environment and Energy, Science and Research Branch, Islamic Azad University, Tehran, I.R. IRAN

3 Department of Chemical Science, Faculty of Science & Technology, North Tehran Branch, Islamic Azad University, Tehran, I.R. IRAN

4 Department of Life Science Engineering, Faculty of New Science & Technology, University of Tehran, Tehran, I.R. IRAN

5 Biotechnology Group, School of Chemical Engineering, College of Engineering, University of Tehran, P.O.Box:11155-4563, Tehran, I.R. IRAN

6 Department of Mycobacteriology and Pulmonary Research, Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, I.R. IRAN

7 Department of Chemical Science, Faculty of Science & Technology, Islamic Azad University North Tehran Branch, Tehran, I.R. IRAN

Abstract

In this study, ectoine is produced by Streptomyces. sp IBRC-M PTCC 10615. Fermentation parameters such as flow regime, gas hold up, mass transfer coefficient, and mixing time were optimized by statistical analysis. Streptomyces. sp produced a maximal ectoine concentration of 270 mmol/kg at optimal conditions of ectoine and L-aspartic acid. Also, the amount of mass transfer, gas hold up, and mixing time were determined 0,41/s ,0.3, and 40 s, respectively. The amount of ectoine was measured by HPLC. Furthermore, Molecular Dynamics (MD) simulation was used for studying the solubility of ectoine in aqueous media. Equilibrium data such as temperature, potential energy, and volume graphs showed that the solubility of ectoine is 25%more than glycerol. Also, all the achieving graphs from the equilibrium of simulation were confirmed the appropriate structure of the system.

Keywords

Main Subjects

References

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Iranian Journal of Chemistry and Chemical Engineering
Volume 39, Issue 6 - Serial Number 104
November and December 2020
Pages 259-269

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