Genotoxicity of Noscapine Nanosuspension Prepared by Microfluidic Reactors on HepG2 Cell Line

Document Type: Research Article


1 Faculty of Biological Sciences, Kharazmi University, Tehran, Iran

2 Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran

3 Department of Medical Nanotechnology, Faculty of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

4 Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran


The present research aimed to investigate the induction of DNA destruction and viability of HepG2 tumor spheroid culture influenced by noscapine and nanosuspention of noscapine. The culture of HepG2 cells as spheroids (mean diameter of 100 μm) was treated with different concentrations of noscapine for 24 h on Day 11. Afterward, viability assay and alkaline comet assay methods, respectively, were applied to examine the viability and induced DNA destructions. Based on the results, no significant impacts of Tween 40 diluent were detected on the viability and DNA damage levels in comparison with the control (p > 0.05). Moreover, increasing noscapine concentration resulted in a dose-dependent reduction in viability cells of hepatic cancer cells and elevated DNA damages showing a correlation between rises of DNA damages and viability decline.


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